Open Access
Research
(Published
online: 05-03-2016)
2.
Peperomia
pellucida
leaf extract as immunostimulator
in controlling motile aeromonad septicemia due to Aeromonas
hydrophila in red hybrid tilapia, Oreochromis spp.
farming -
S. W. Lee,
K. Y. Sim,
W. Wendy
and A. K. Zulhisyam
Veterinary World, 9(3): 231-234
doi:
10.14202/vetworld.2016.231-234
S. W. Lee :
Faculty of Agro
Based Industry, Universiti Malaysia Kelantan Jeli Campus, 17600,
Jeli, Kelantan, Malaysia; leeseongwei@yahoo.com
K. Y. Sim :
Faculty of Agro
Based Industry, Universiti Malaysia Kelantan Jeli Campus, 17600,
Jeli, Kelantan, Malaysia; sk.yuen@umk.edu.my
W. Wendy :
School of Fisheries
and Aquaculture Sciences, Universiti Malaysia Terengganu, Kuala
Terengganu, 21030, Terengganu, Malaysia; wendy@umt.edu.my
A. K. Zulhisyam :
Faculty of Agro
Based Industry, Universiti Malaysia Kelantan Jeli Campus, 17600,
Jeli, Kelantan, Malaysia; zulhisyam.a@umk.edu.my
Received: 06-06-2015, Revised: 08-01-2016, Accepted: 23-01-2016,
Published online: 05-03-2016
Corresponding author:
S. W. Lee, e-mail: leeseongwei@yahoo.com
Citation:
Lee SW, Sim KY, Wendy W, Zulhisyam AK (2016) Peperomia
pellucida leaf extract as immunostimulator in controlling
motile aeromonad septicemia due to Aeromonas hydrophila
in red hybrid tilapia, Oreochromis spp. farming,
Veterinary World, 9(3): 231-234.
Abstract
Aim:
This study
was revealed the potential of Peperomia pellucida leaf
extract as an immunostimulator agent in controlling motile
aeromonad septicemia due to Aeromonas hydrophila in red
hybrid tilapia, Oreochromis sp.
Materials and
Methods:
In the present
study, minimum inhibitory concentration (MIC) of P. pellucida
leaf extract against A. hydrophila was determined
through two-fold microbroth dilution method. The plant extract
was screening for its active compound using a gas chromatograph
mass spectrometer, and the effectiveness of P. pellucida
leaf extract as an immunostimulator agent was evaluated. The
experimental fish were fed with medicated feed at three
different concentrations (25 mg/kg, PP-25; 50 mg/kg, PP-50; and
100 mg/kg, PP-100) of P. pellucida leaf extract for 1
week before they were intraperitoneally exposed to A.
hydrophila. Enzyme-linked immunosorbent assay was carried
out to determine the value of antibody response to A.
hydrophila in fish from a group of fish that received
medicated feed, and the percentage of total cumulative mortality
of the experimental fish were observed at the end of the
experiment.
Results:
The
results showed that the major bioactive compound is phytol
(40%), and the MIC value was 31.5 mg/L. The value of antibody
response to A. hydrophila in fish from a group of fish
which received medicated feed (PP-25, 0.128±0.014 optical
density [OD]; PP-50, 0.132±0.003 OD; and PP-100, 0.171±0.02 OD)
was found significantly higher (p<0.05) compared to fish did not
receive medicated feed (0.00 OD). Whereas, percentage cumulative
mortality of fish from all groups of fish received medicated
feed (PP-25, 18.0±3.2%; PP-50, 18.2±2.8%; and PP-100, 17.7±1.8%)
were found significantly lower (p<0.05) compared to a group of
fish did not receive medicated feed (83.2±1.4%).
Conclusion:
The
findings of the present study indicated the huge potential of
P. pellucida leaf extract as natural immunostimulator agent
for aquaculture uses.
Keywords:
Aeromonas hydrophila,
immunostimulator, motile aeromonad septicemia, red hybrid
tilapia.
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