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Research (Published online: 02-05-2016)

1. Minimization of apoptosis-like changes in cryopreserved buffalo bull sperm by supplementing extender with Bcl-2 protein - Jasmer Dalal, Ajeet Kumar, Mrigank Honparkhe, Dipak Deka and Narinder Singh

Veterinary World, 9(5): 432-436

 

 

   doi: 10.14202/vetworld.2016.432-436

 

 

Jasmer Dalal: Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India; jasmer.sl.dalal@gmail.com

Ajeet Kumar: Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India; ajeetvet@yahoo.com

Mrigank Honparkhe: Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India; honparkhem@rediffmail.com

Dipak Deka: School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India; dekadipak@rediffmail.com

Narinder Singh: Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India; narinder2002us@yahoo.com

 

Received: 28-01-2016, Accepted: 16-04-2016, Published online: 02-05-2016

 

Corresponding author: Ajeet Kumar, e-mail: ajeetvet@yahoo.com


Citation: Dalal J, Kumar A, Honparkhe M, Deka D, Singh N (2016) Minimization of apoptosis-like changes in cryopreserved buffalo bull sperm by supplementing extender with Bcl-2 protein, Veterinary World, 9(5): 432-436.



Aim: This study was aimed at evaluating the anti-apoptotic effects of Bcl-2 protein in cryopreserved buffalo bull sperm.

Materials and Methods: A total 10 ejaculates from two buffalo bulls (5 each) were collected using artificial vagina method, and semen was evaluated using a standard protocol. Semen was extended by Tris egg yolk extender supplemented with Bcl-2 protein at 5, 10, and 15 μM. Semen was cryopreserved at ultra-low temperature using traditional vapor freezing method. Prefreeze and post-thaw semen samples were evaluated for percent motility, viability, hypo-osmotic swelling test (HOST) reactive sperms; status of mitochondrial membrane activity and status of sperm phospholipase A1 and phospholipase A2 activity.

Results: There were no significant effects of Bcl-2 protein supplementation on pre-freeze sperm quality. Percent motility and active mitochondria in post-thaw Bcl-2 supplemented and control groups were also similar. However, viable sperms were significantly (p<0.05) higher (74.29±4.23%) in Bcl-2 supplemented group (5 μM) as compared to control (51.6±5.77%). The proportion of HOST reactive sperms was also higher (63.1±6.73%) in Bcl-2 supplemented (5 μM) group as compared to control (50.7±6.98%). The sperm with low PLA activity (non-apoptotic) was significantly (p<0.05) higher in all the supplemented doses of Bcl-2 protein, i.e., at 5 μM (73.42±5.79%), 10 μM (75.51±6.22%), and 15 μM (74.78±5.89%) as compared to control (60.23±4.45%). We found that Bcl-2 protein supplementation at 5 μM dose improved the postthaw semen quality indicated by higher viability, HOST reactive sperms, and sperm with low PLA activity (non-apoptotic sperms).

Conclusion: Bcl-2 protein supplementation exerts its protective effect on spermatozoa against apoptosis-like changes developed during cryopreservation.

Keywords: anti-apoptotic effects, Bcl-2 protein, buffalo bull, semen cryopreservation.



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