Open Access
Research
(Published
online: 24-05-2016)
13.
Studies of the macroscopic and microscopic
morphology (hippocampus) of brain in Vencobb broiler -
Shailesh Kumar Gupta, Kumaresh Behera, C. R. Pradhan, Arun Kumar
Mandal, Kamdev Sethy, Dayanidhi Behera and Kuladip Prakash
Shinde
Veterinary World, 9(5): 507-511
doi:
10.14202/vetworld.2016.507-511
Shailesh Kumar Gupta:
Department of Livestock Production and Management, College of
Veterinary Sciences and Animal Husbandry, Bhubaneswar - 751 003,
Odisha, India; sgshailesh786@gmail.com
Kumaresh Behera:
Department of Livestock Production and Management, College of
Veterinary Sciences and Animal Husbandry, Bhubaneswar - 751 003,
Odisha, India; kumaresh.behera@gmail.com
C. R. Pradhan:
Department of Livestock Production and Management, College of
Veterinary Sciences and Animal Husbandry, Bhubaneswar - 751 003,
Odisha, India; pradhancr@gmail.com
Arun Kumar Mandal:
Department of Veterinary Anatomy and Histology, College of
Veterinary Sciences and Animal Husbandry, Bhubaneswar - 751 003,
Odisha, India; arunmandal_2005yahoo.com
Kamdev Sethy:
Department of Animal Nutrition, College of Veterinary Sciences
and Animal Husbandry, Bhubaneswar - 751 003, Odisha, India;
babuivri@gmail.com
Dayanidhi Behera:
Department of Livestock Production and Management, College of
Veterinary Sciences and Animal Husbandry, Bhubaneswar - 751 003,
Odisha, India; phddaya@gmail.com
Kuladip Prakash Shinde:
Livestock Production Management Section, ICAR - National Dairy
Research Institute (NDRI), Karnal - 132 001, Haryana, India;
kuls164@gmail.com
Received: 22-01-2016, Accepted: 16-04-2016, Published online:
24-05-2016
Corresponding author:
Shailesh Kumar Gupta, e-mail: sgshailesh786@gmail.com
Citation:
Gupta SK, Behera K, Pradhan CR, Mandal AK, Sethy K, Behera D,
Shinde KP (2016) Studies of the macroscopic and microscopic
morphology (hippocampus) of brain in Vencobb broiler,
Veterinary World, 9(5): 507-511.
Abstract
Aim:
The aim of this study was to study the anatomy of different
parts of brain and histology of hippocampus of Vencobb broiler
chicken.
Materials and Methods:
A 12 adult experimental birds were sacrificed by cervical
dislocation. After separation of the brain, gross anatomy
features were studied. Brain tissue was fixed in 10% buffered
neutral formalin for 2-3 days, and then routine dehydration
process in ascending grades of ethyl alcohol was done. After
xylene cleaning, paraffin impregnation was prepared. Paraffin
blocks were cut, and slides were stained by Harris hematoxylin
and eosin. Photography was carried out both under lower (×10)
and higher (×40) magnifications.
Results:
The brain structure (dorsal view) of Vencobb bird resembled the
outline of a playing card symbol of a “spade.” The brain
subdivisions are cerebrum, cerebellum, and medulla oblongata.
Cerebrum was devoid of usual convolutions (elevations), gyri,
depressions (grooves), and sulci. The cerebral hemispheres were
tightly apposed along a median sulcus called interhemispheric
fissure and cerebrum and cerebellum were separated by a small
transverse fissure. The olfactory bulb was small structures, and
the pineal body was clearly visible. The optic lobes were
partially hidden under cerebral hemispheres, but laterally, it
was large, prominent rounded or spherical bodies of the
midbrain. The hippocampal area appeared as dorso-medial
protrusion. Different types of neurons were distinguished in the
hippocampus were pyramidal neurons, pyramidal-like neurons, and
multipolar neurons, etc. There was rich vascularization in the
form of blood capillaries throughout the hippocampus.
Conclusion:
Cerebrum was pear shaped and largest part of the brain. Cerebrum
hemisphere was smooth devoid of convolutions, gyri, and
depressions, but in the surface of cerebellum, there was the
presence of a number of transverse depression (grooves) and
sulci subdividing into many folds. Olfactory bulb was poorly
developed, whereas optic lobes were rounded and large. The exact
boundary line of the hippocampus was not discernable. In
hippocampus histology, two categories of neuron local circuit
neurons and projection neurons, high vascularization and
epididymal lining of lateral ventricle were observed.
Hippocampal neurons were comparatively larger without any
distinct layers. The afferent neurons projected to the medium
septum.
Keywords:
capillaries, hemisphere, hippocampus, neurons, Vencobb broiler.
References
1. Herculano-Houzel, S. (2011) Brains matter, bodies may be
not: The case for examining neuron numbers irrespective of
body size. Ann. N.Y. Acad. Sci., 1225: 191-199.
http://dx.doi.org/10.1111/j.1749-6632.2011.05976.x
PMid:21535005 |
|
|
|
2. Abd-Alrahman, S.A. (2012) Morphological and histological
study of the cerebrum in a nocturnal bird species (Barn Owl)
Tyto alba. Ibn Al-Haitham J. Pure Appl. Sci., 25(3): 73-87. |
|
|
3. Frahm, H.D. and Rehkamper, G. (1998) Allometric
comparison of the brain and brain structures in the white
crested polish chicken with uncrested domestic chicken.
Brain Behav. Evol., 52: 292-307.
http://dx.doi.org/10.1159/000006574
PMid:9807014 |
|
|
4. Kawabe, S., Shimokawa, T., Miki, H., Matsuda, S. and
Endo, H. (2013) Variation in avian brain shape: Relationship
with size and orbital shape. J. Anat., 223(5): 495-508.
http://dx.doi.org/10.1111/joa.12109 |
|
|
|
5. Dhage, S.A., Shehan, N.A., Ali, S.A. and Aziz, F.H.
(2013) Anatomical and histological study of cerebral in
Sturnus vulgaris. Bas. J. Vet. Res., 12(2): 221-227. |
|
|
|
6. Peng, K., Feng, Y., Zhang, G, Liu, H. and Song, H. (2010)
Anatomical study of the brain of the African ostrich. Turk.
J. Vet. Anim. Sci., 34(3): 235-241. |
|
|
7. Srivastava, U.C., Chand, P. and Maurya, R.C. (2007)
Cytoarchitectonic organization and morphology of the cells
of hippocampal complex in Strawberry Finch, Estrilda
amandava. Cell. Mol. Biol., 53(5): 103-120.
PMid:17543239 |
|
|
8. Tombol, T., Davies, D.C., Németh, A., Alpár, A. and
Sebestény, T. (2000) A Golgi and a combined Golgi/GABA
immunogold study of local circuit neurons in the homing
pigeon hippocampus. Anat. Embryol., 201: 181-196.
http://dx.doi.org/10.1007/s004290050017 |
|
|
|
9. Bancroft, J.D., Stevens, A. and Turner, D.R. (1996)
Theory and Practice of Histological Techniques. 4th ed.
Churchill Livingstone, New York. p99-112. |
|
|
|
10. Batah, A.L., Ghaje, M.S. and Aziz, S.N. (2012)
Anatomical and Histological study for the brain of the
locally breed chicken. J. Thi-Qar Sci., 3(3): 47-53. |
|
|
|
11. Husband, S. and Shimizu, T. (1999) Evolution of the
avian visual system. Available from: http://www.luna.cas.usf.edu/~husband/evolve/default.htm. |
|
|
12. Sultan, F. (2005) Why some bird brains are larger than
others. Curr. Biol., 15: 649-650.
http://dx.doi.org/10.1016/j.cub.2005.08.043
PMid:16139191 |
|
|
|
13. Krebs, J.R., Clayton, N.S., Healy, S.D., Cristol, D.A.,
Patel, S.N. and Jolliffe, A.R. (1996) The ecology of the
avian brain: Food-storing memory and the hippocampus. Int.
J. Avian Sci., 138(1): 34-46. |
|
|
14. Patzke, N., Ocklenburg, S., vanderStaay, F.J., Gunturkun,
O. and Manns, M. (2009) Consequences of different housing
conditions on brain morphology in laying hens. J. Chem.
Neuroanat., 37: 141-148.
http://dx.doi.org/10.1016/j.jchemneu.2008.12.005
PMid:19135145 |
|
|
15. Liu, X., Ramirez, S., Pang, P.T., Puryear, C.B.,
Govindarajan, A., Deisseroth, K. and Tonegawa, S. (2012)
Optogenetic stimulation of a hippocampal engram activates
fear memory recall. Nature, 484: 381-385.
http://dx.doi.org/10.1038/nature11028 |
|
