Open Access
Research
(Published
online: 27-05-2016)
15.
Effect of washing on the post-thaw quality
of cryopreserved ram epididymal spermatozoa -
Touqeer Ahmed, Rafiqul Islam, Farooz Ahmad Lone and Asloob Ahmad
Malik
Veterinary World, 9(5): 519-523
doi:
10.14202/vetworld.2016.519-523
Touqeer Ahmed:
Division of Animal Reproduction, Gynaecology and Obstetrics,
Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir
University of Agricultural Sciences & Technology of Kashmir,
Shuhama, Alusteng, Srinagar, Jammu & Kashmir, India; touqeer_taz@yahoo.co.in
Rafiqul Islam:
Division of Animal Reproduction, Gynaecology and Obstetrics,
Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir
University of Agricultural Sciences & Technology of Kashmir,
Shuhama, Alusteng, Srinagar, Jammu & Kashmir, India; rafiqvet@gmail.com
Farooz Ahmad Lone:
Division of Animal Reproduction, Gynaecology and Obstetrics,
Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir
University of Agricultural Sciences & Technology of Kashmir,
Shuhama, Alusteng, Srinagar, Jammu & Kashmir, India;
dr.farooz462@gmail.com
Asloob Ahmad Malik:
Division of Animal Reproduction, Gynaecology and Obstetrics,
Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir
University of Agricultural Sciences & Technology of Kashmir,
Shuhama, Alusteng, Srinagar, Jammu & Kashmir, India; malikasloob@gmail.com
Received: 01-02-2016, Accepted: 20-04-2016, Published online:
27-05-2016
Corresponding author:
Touqeer Ahmed, e-mail: touqeer_taz@yahoo.co.in
Citation:
Ahmed T, Islam R, Lone FA, Malik AA (2016) Effect of washing on
the post-thaw quality of cryopreserved ram epididymal
spermatozoa, Veterinary World, 9(5): 519-523.
Abstract
Aim:
The aim of the study was to evaluate the effect of washing on
the post-thaw quality of ram cauda epididymal spermatozoa (P1:
Unwashed, P2: Washed).
Materials and Methods:
Fresh testicles of adult healthy slaughtered rams were collected
and transported to the laboratory in an ice chest, where they
were weighed, and cauda epididymides were separated. These cauda
epididymides were used for recovery of spermatozoa in tris-citric
acid fructose buffer by incision method. Spermatozoa samples
showing ≥70% progressive motility were pooled and processed
further. The mean values (±standard error) of various parameters
such as the percentage of sperm motility, live sperm, intact
acrosome, and hypo-osmotic swelling test (HOST) rea cted
spermatozoa were recorded.
Results:
In this experiment, the percent sperm motility, live
spermatozoa, and intact acrosome both at pre-freeze and postthaw
were higher (p>0.05) in P1 than P2. However, the post-thaw
percent HOST reacted spermatozoa was slightly higher (p>0.05)
for P2 than P1.
Conclusion:
Washing of cauda epididymal spermatozoa has no significant
adverse effect on the quality during cryopreservation.
Therefore, this processing method can be applied wherever
necessary before the extension of the recovered spermatozoa
sample in different ram extenders.
Keywords:
cauda epididymis, centrifugation, processing, ram, spermatozoa,
washing.
References
1. Behera, S., Harshan, H.M., Bhai, K.L. and Ghosh, K.N.A.
(2015) Effect of cholesterol supplementation on cryosurvival
of goat spermatozoa. Vet. World, 8: 1386-1391.
http://dx.doi.org/10.14202/vetworld.2015.1386-1391
PMid:27047048 PMCid:PMC4774814 |
|
|
2. Ali, A.T., Bomboi, G. and Floris, B. (2013) Replacing
chicken yolk with yolks from other sources in ram semen
diluents and their effects on fertility in vitro. Small Rum.
Res., 113: 405-410.
http://dx.doi.org/10.1016/j.smallrumres.2013.01.017 |
|
|
|
3. Naing, S.W., Haron, A.W., Goriman, M.A.K., Yusoff, R.,
Abu Bakar, M.Z., Sarsaifi, K., Bukar, M.M., Thein, M., Kyaw,
T. and San M.M. (2011) Effect of seminal plasma removal,
washing solutions, and centrifugation regimes on boer goat
semen cryopreservation. Pertanika J. Trop. Agric. Sci.,
34(2): 271-279. |
|
|
4. Forouzanfar, M., Ershad, S.F., Hosseini, S.M., Hajian,
M., Hosseini, S.O., Abid, A., Tavalaee, M., Shahverdi, A.,
Dizaji, A.V. and Esfahani, M.H.N. (2013) Can permeable super
oxide dismutase mimetic agents improve the quality of
frozen–thawed ram semen. Cryobiology, 66: 126-130.
http://dx.doi.org/10.1016/j.cryobiol.2012.12.006
PMid:23276547 |
|
|
5. Azeredo, G.A., Esper, C.R. and Resende, K.T. (2001)
Evaluation of plasma membrane integrity of frozen-thawed
goat spermatozoa with or without seminal plasma. Small Rum.
Res., 41(3): 257-263.
http://dx.doi.org/10.1016/S0921-4488(01)00189-4 |
|
|
6. Peterson, K., Kappen, M.A.P., Ursem, P.J.F., Nothling,
J.O., Colenbrander, B. and Gadella, B.M. (2007) Microscopic
and flow cytometric semen assessment of Dutch AI-bucks:
Effect of semen processing procedures and their correlation
to fertility. Theriogenology, 67(4): 863-871.
http://dx.doi.org/10.1016/j.theriogenology.2006.11.003
PMid:17126897 |
|
|
7. Kozdrowski, R., Dubiel, A., Bielas, W. and Dzieciol, M.
(2007) Two protocols of cryopreservation of goat semen with
the use of computer-analysis system. Acta Vet. Brno., 76:
601-604.
http://dx.doi.org/10.2754/avb200776040601 |
|
|
8. Sarıozkan, S., Bucak, M.N., Tuncer, P.B., Tasdemir, U.,
Kinet, H. and Ulutas, P.A. (2010) Effects of different
extenders and centrifugation/washing on post thaw
microscopic-oxidative stress parameters and fertilizing
ability of Angora buck sperm. Theriogenology, 73: 316-323.
http://dx.doi.org/10.1016/j.theriogenology.2009.09.015
PMid:19880169 |
|
|
9. Carvajal, G., Cuello, C., Ruiz, M., Vazquez, J.M.,
Martinez, E.A. and Roca, J. (2004) Effect of centrifugation
on Boar sperm. J. Androl., 25: 389-396.
http://dx.doi.org/10.1002/j.1939-4640.2004.tb02805.x
PMid:15064317 |
|
|
10. Webb, G.W. and Dean, M.M. (2009) Effect of
centrifugation technique on post-storage characteristics of
stallion spermatozoa. J. Equine Vet. Sci., 29(9): 675-680.
http://dx.doi.org/10.1016/j.jevs.2009.07.016 |
|
|
11. Purdy, P.H. (2006) A review on goat sperm
cryopreservation. Small Rum. Res., 63(3): 215-225.
http://dx.doi.org/10.1016/j.smallrumres.2005.02.015 |
|
|
|
12. Lone, F.A., Islam, R., Khan, M.Z. and Sofi, K.A. (2011b)
Effect of collection methods on the quality and quantity of
spermatozoa recovered from the cauda epididymidis of
slaughtered ram. Indian Vet. J., 88(9): 46-48. |
|
|
13. Lone, F.A., Islam, R., Khan, M.Z. and Sofi, K.A. (2012)
Effect of different egg yolk-based extenders on the quality
of ovine cauda epididymal spermatozoa during storage at 4°C.
Reprod. Domest. Anim., 47: 257-262.
http://dx.doi.org/10.1111/j.1439-0531.2011.01847.x
PMid:21883509 |
|
|
14. Zemjanis, R. (1970) Diagnostic and Therapeutic
Techniques in Animal Reproduction. 2nd ed. Williams and
Willkins Co., Baltimore.
PMCid:PMC2047459 |
|
|
15. Foote, R.H. (1970) Influence of extender, extension rate
and glycerolating technique on fertility of frozen bull
semen. J. Dairy Sci., 53: 1478-1482.
http://dx.doi.org/10.3168/jds.S0022-0302(70)86418-9 |
|
|
|
16. Blom, E. (1977) Sperm morphology with reference to bull
infertility. In: Some Papers Contributed to the 1st All
India Symposium on Animal Reproduction. Punjab Agricultural
University, Ludhiana, India, p66-81. |
|
|
17. Watson, P.F. (1975) Use of Giemsa stain to detect
changes in acrosomes of frozen ram spermatozoa. Microsc.
Res. Tech., 61: 1-6.
http://dx.doi.org/10.1136/vr.97.1.12 |
|
|
18. Vasquez, J., Florentini, E.A., Camargo, L.A., Gonzales,
J. and Valdivia, M. (2013) Hypo osmotic swelling test in
epididymal ram (Ovis aries) spermatozoa. Livest. Sci., 157:
618-622.
http://dx.doi.org/10.1016/j.livsci.2013.08.023 |
|
|
19. Hoogewijs, M., Rijsselaere, T., De Vliegher, S.,
Vanhaesebrouck, E., De Schauwer, C., Govaere, J., Thys, M.,
Hoflack, G., Soom, A.V. and de Kruif, A. (2010) Influence of
different centrifugation protocols on equine semen
preservation. Theriogenology, 74(1): 118-126.
http://dx.doi.org/10.1016/j.theriogenology.2010.01.022
PMid:20207406 |
|
|
20. Islam, R., Ahmed, K. and Deka, B.C. (2006) Effect of
holding and washing on the quality of goat semen. Small Rum.
Res., 66: 51-57.
http://dx.doi.org/10.1016/j.smallrumres.2005.06.034 |
|
|
21. Jones, R.C. and Holt, W.V. (1974) The effect of washing
on the ultra structure and cytochemistry of ram spermatozoa.
J. Reprod. Fertil., 41(1): 159-167.
http://dx.doi.org/10.1530/jrf.0.0410159 |
|
|
|
22. Islam, R. and Ahmed, K. (2003) Effect of seminal plasma
and of its removal on the quality of goat semen during
preservation at 5°C. Indian Vet. J., 80: 1302-1303. |
|
|
23. Lone, F.A., Islam, R., Khan, M.Z. and Sofi, K.A. (2011a)
Effect of transportation temperature on the quality of cauda
epididymal spermatozoa of ram. Anim. Reprod. Sci., 123:
54-59.
http://dx.doi.org/10.1016/j.anireprosci.2010.10.012
PMid:21131148 |
|
