Open Access
Research
(Published
online: 09-05-2016)
5.
Single nucleotide polymorphisms
in toll-like receptor genes and case-control association studies
with bovine tuberculosis
-
Ashish Bhaladhare, Deepak Sharma, Amit Kumar, Arvind Sonwane,
Anuj Chauhan, Ranvir Singh, Pushpendra Kumar, Ramji Yadav, Mohd
Baqir, Bharat Bhushan and Om Prakash
Veterinary World, 9(5): 458-464
doi:
10.14202/vetworld.2016.458-464
Ashish Bhaladhare:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; abhaladhare@gmail.com
Deepak Sharma:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; anuj_vet99@rediffmail.com
Amit Kumar:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; vetamitchandan07@gmail.com
Arvind Sonwane:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; arvind2002@gmail.com
Anuj Chauhan:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; anuj_vet99@rediffmail.com
Ranvir Singh:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; rvsingh.icar@gmail.com
Pushpendra Kumar:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; pushpendra64@gmail.com
Ramji Yadav:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; drramjivet@gmail.com
Mohd Baqir:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; drbaqirvet@gmail.com
Bharat Bhushan:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; bbhushan_ivri2003@yahoo.co.in
Om Prakash:
Division of
Animal Genetics, Indian Veterinary Research Institute, Izatnagar,
Uttar Pradesh, India; dromprakash0511@gmail.com
Received: 06-10-2015, Accepted: 21-03-2016, Published online:
09-05-2016
Corresponding author:
Anuj Chauhan, e-mail: anuj_vet99@rediffmail.com
Citation:
Bhaladhare A, Sharma D, Kumar A, Sonwane A, Chauhan A, Singh R,
Kumar P, Yadav R, Baqir M, Bhushan B, Prakash O (2016) Single
nucleotide polymorphisms in toll-like receptor genes and
case-control association studies with bovine tuberculosis,
Veterinary World, 9(5): 458-464.
Abstract
Aim:
Toll-like
receptor 2 (TLR2) and TLR4 genes play critical roles in host
recognition of Mycobacterium bovis infection and
initiation of innate and adaptive immune response. The present
study was aimed at exploring the association of seven single
nucleotide polymorphisms (SNPs) in TLR2 and TLR4 genes with
susceptibility/resistance against bovine tuberculosis (bTB)
infection in cattle.
Materials and
Methods:
A case-control
resource population of 35 positive and 45 negative animals was
developed after screening with single intradermal tuberculin
test for bTB. Resource population was screened for SNPs in TLR2
and TLR4 genes using polymerase chain reaction-restriction
fragment length polymorphism. The PROC LOGISTIC procedure of SAS
9.3 was used to find an association of allelic and genotypic
frequencies with bTB.
Results:
In
TLR2 gene, two of SNPs under study (rs55617172 and rs68268253)
revealed polymorphism while in the case of TLR4 gene all four
SNPs under investigation (rs8193041, rs207836014, rs8193060, and
rs8193069) were found to be polymorphic in case-control
population. SNP locus rs55617172 in TLR2 gene was found
significantly (p<0.01) associated with susceptibility/resistance
to TB in cattle.
Conclusion:
These
findings indicate the presence of SNPs in TLR2 and TLR4 genes in
our resource population. Upon validation in independent, large
resource population and following biological characterization,
SNP rs55617172 can be incorporated in marker panel for selection
of animals with greater resistance to bTB.
Keywords:
bovine tuberculosis, immune response, resistance, single
nucleotide polymorphisms, toll-like receptors.
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