Open Access
Review
(Published
online: 11-05-2016)
6.
Loop mediated isothermal amplification: An
innovative gene amplification technique for animal diseases -
Pravas Ranjan Sahoo, Kamadev Sethy, Swagat Mohapatra and Debasis
Panda
Veterinary World, 9(5): 465-469
doi:
10.14202/vetworld.2016.465-469
Pravas Ranjan Sahoo:
Department of Veterinary Biochemistry, Odisha University of
Agriculture & Technology, Bhubaneswar, Odisha, India;
pravasvet86@gmail.com
Kamadev Sethy:
Department of Animal Nutrition, Odisha University of Agriculture
& Technology, Bhubaneswar, Odisha, India; babuivri@gmail.com
Swagat Mohapatra:
Department of Veterinary Physiology, Odisha University of
Agriculture & Technology, Bhubaneswar, Odisha, India;
swagat.physiology@gmail.com
Debasis Panda:
Department of Veterinary Physiology, Odisha University of
Agriculture & Technology, Bhubaneswar, Odisha, India; bapu8280@mail.com
Received: 23-02-2016, Accepted: 05-04-2016, Published online:
11-05-2016
Corresponding author:
Pravas Ranjan Sahoo, e-mail: pravasvet86@gmail.com
Citation:
Sahoo PR, Sethy K, Mohapatra S, Panda D (2016) Loop mediated
isothermal amplification: An innovative gene amplification
technique for animal diseases,
Veterinary World, 9(5):
465-469.
Abstract
India being a developing country mainly depends on livestock
sector for its economy. However, nowadays, there is emergence
and reemergence of more transboundary animal diseases. The
existing diagnostic techniques are not so quick and with less
specificity. To reduce the economy loss, there should be a
development of rapid, reliable, robust diagnostic technique,
which can work with high degree of sensitivity and specificity.
Loop mediated isothermal amplification assay is a rapid gene
amplification technique that amplifies nucleic acid under an
isothermal condition with a set of designed primers spanning
eight distinct sequences of the target. This assay can be used
as an emerging powerful, innovative gene amplification
diagnostic tool against various pathogens of livestock diseases.
This review is to highlight the basic concept and methodology of
this assay in livestock disease.
Keywords:
designed primers, loop-mediated isothermal amplification assay,
transboundary animal diseases.
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