Open Access
Research
(Published
online: 17-11-2016)
16.
Inhibition of bovine platelets aggregation in response to
Hyalomma anatolicum
salivary gland proteins/peptides -
Surbhi, Nirmal Sangwan, Arun K. Sangwan, Vijender Singh and Ankit
Kumar
Veterinary World, 9(11): 1264-1268
doi:
10.14202/vetworld.2016.1264-1268
Surbhi:
Department of Veterinary Physiology and Biochemistry, Lala
Lajpat Rai University of Veterinary & Animal Sciences, Hisar -
125 004, Haryana, India; surbhi804@gmail.com
Nirmal Sangwan:
Department of Veterinary Physiology and Biochemistry, Lala Lajpat
Rai University of Veterinary & Animal Sciences, Hisar - 125 004,
Haryana, India; nirmalsangwan@gmail.com
Arun K. Sangwan:
Department of Veterinary Parasitology, Lala Lajpat Rai University
of Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India;
sangwan_arun@hotmail.com
Vijender Singh:
Department of Veterinary Physiology and Biochemistry, Lala Lajpat
Rai University of Veterinary & Animal Sciences, Hisar - 125 004,
Haryana, India; vijender@luvas.edu.in
Ankit Kumar:
Department of Veterinary Medicine, Lala Lajpat Rai University of
Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India;
ankitk813@gmail.com
Received: 11-06-2016, Accepted: 07-10-2016, Published online:
17-11-2016
Corresponding author:
Nirmal Sangwan, e-mail: nirmalsangwan@gmail.com
Citation:
Surbhi, Sangwan N, Sangwan AK, Singh V, Kumar A (2016) Inhibition
of bovine platelets aggregation in response to
Hyalomma anatolicum
salivary gland proteins/peptides,
Veterinary World, 9(11):
1264-1268.
Abstract
Aim:
Ticks are obligate ectoparasites that have an impact on wide range
of vertebrates and also act as a potential vector for the
transmission of tropical theileriosis, babesiosis, etc., causing
significant loss to livestock production worldwide. While feeding,
they introduce their saliva containing different bioactive
molecules into the host. These molecules have the capability to
counteract the host hemostatic mechanism to suck host blood
successfully. Therefore, the study was aimed to isolate
anti-platelet aggregating peptides from salivary gland extract (SGE)
of
Hyalomma anatolicum
ticks, a commonly available tick in India.
Materials and Methods:
Female
H. anatolicum
salivary glands were dissected out and SGE was prepared by
homogenizing it in a suitable buffer under ice. Extract so
obtained was fractionated by gel filtration chromatography using
Sephacryl S-200 column. Total protein concentration in fractions
was estimated and bovine platelets were isolated, stimulated with
thrombin (positive control), treated with Gly-Pro-Arg-Pro amide
(negative control) and with salivary gland fractions for
identification of proteins/peptides having anti-platelet
aggregating activities.
Results:
Proteins/peptides present in various salivary gland fractions
inhibited the bovine platelet aggregation and the percent
inhibition ranged between 33% and 35.8%.
Conclusion:
The results suggests that the fractions of
H. anatolicum
salivary glands possess thrombin-induced anti-platelet aggregating
activity and which could be further exploited for raising
anti-tick vaccine and also for therapeutic purpose.
Keywords:
anti-platelet aggregating proteins/peptides, gel filtration
chromatography,
Hyalomma anatolicum
salivary gland, thrombin.
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