Open Access
Research
(Published
online: 25-11-2016)
21.
Allelic and genotypic frequencies in
polymorphic Booroola fecundity gene and their association with
multiple birth and postnatal growth in Chhotanagpuri sheep
-
Thanesh Oraon, D. K. Singh, Mayukh Ghosh, S. S. Kullu, Rajesh
Kumar and L. B. Singh
Veterinary World, 9(11): 1294-1299
doi:
10.14202/vetworld.2016.1294-1299
Thanesh Oraon:
Department of Animal Breeding and Genetics, Ranchi Veterinary
College, Birsa Agricultural University, Kanke, Ranchi, Jharkhand,
India; thaneshoraonvet@gmail.com
D. K. Singh:
Department of Animal Breeding and Genetics, Ranchi Veterinary
College, Birsa Agricultural University, Kanke, Ranchi, Jharkhand,
India; dksinghdron_bau@rediffmail.com
Mayukh Ghosh:
Department of Veterinary Biochemistry, Ranchi Veterinary College,
Birsa Agricultural University, Kanke, Ranchi, Jharkhand, India;
ghosh.mayukh87@gmail.com
S. S. Kullu:
Department of Animal Nutrition, Ranchi Veterinary College, Birsa
Agricultural University, Kanke, Ranchi, Jharkhand, India;
kullusingray@gmail.com
Rajesh Kumar:
Department of Veterinary Physiology, Veterinary College, Pookode,
Lakkidi, Kerala, India; drrajesh7.vet@gmail.com
L. B. Singh:
Department of Animal Breeding and Genetics, Ranchi Veterinary
College, Birsa Agricultural University, Kanke, Ranchi, Jharkhand,
India; lbsingh.bau.ranchi@gmail.com
Received: 11-06-2016, Accepted: 15-10-2016, Published online:
25-11-2016
Corresponding author:
Thanesh Oraon, e-mail: thaneshoraonvet@gmail.com
Citation:
Oraon T, Singh DK, Ghosh M, Kullu SS, Kumar R, Singh LB (2016)
Allelic and genotypic frequencies in polymorphic Booroola
fecundity gene and their association with multiple birth and
postnatal growth in chhotanagpuri sheep,
Veterinary World, 9(11):
1294-1299.
Abstract
Aim:
Chhotanagpuri breed of sheep reared for mutton in Jharkhand,
India, having problem of low litter size and body weight. The
response of genetic improvement for traits with low heritability
through traditional selection method is timeconsuming. Therefore,
marker-assisted selection based on a polymorphism study of
suitable candidate gene can response quickly. Thus, this study was
aimed at identification of different allelic and genotypic
frequencies of Booroola fecundity (FecB)
gene and its association with multiple birth and postnatal growth
in Chhotanagpuri sheep.
Materials and Methods:
DNA isolation and gene-specific amplification of
FecB
gene was performed from blood samples of from 92 Chhotanagpuri
lambs maintained under similar feeding and management conditions.
Custom nucleotide sequencing and single-strand conformational
polymorphism analysis were performed to identify different
genotypes with respect to the target gene. Statistical analysis
was performed for determination of allelic and genotypic
frequencies of
FecB
gene polymorphisms and its association with multiple birth and
postnatal growth of lambs from birth to 52 weeks age.
Results:
“AA” “AB” and “BB” genotypes were found at locus-1 as it is
polymorphic for
FecB
gene while locus-2 was found to be monomorphic for
FecB
gene. Higher frequency of “A” allele at locus-1 was found in
single born lambs, whereas “B” allele was predominant among
multiple born lambs. The lambs having “BB” genotype weighed
significantly (p≤0.01) heavier than those of “AB” and “AA”
genotype at 52 weeks of age.
Conclusion:
“BB” genotype has emerged as favored genotype for multiple births
and better growth indicator. Therefore, homozygous lambs for “B”
allele should be selected and utilized in breeding program for
better growth rate.
Keywords:
body growth, Booroola fecundity gene, Chhotanagpuri sheep,
multiple births, single-strand conformational
polymorphism.
References
1. Naqvi, S.M.K., Sejian, V. and Karim, S.A. (2013) Effect of
feed flushing during summer season on growth, reproductive
performance and blood metabolites in Malpura ewes under
semi-arid tropical environment. Trop. Anim. Health Prod., 45:
143-148.
https://doi.org/10.1007/s11250-012-0185-2
PMid:22669488 |
|
|
2. Miao, X. and Luo, Q. (2013) Genome-wide transcriptome
analysis between small-tail Han sheep and the Surabaya fur
sheep using high-throughput RNA sequencing. Reproduction, 145:
587-596.
https://doi.org/10.1530/REP-12-0507
PMid:23579189 |
|
|
|
3. Dekkers, J.C.M. (2004) Commercial application of marker and
gene-assisted selection in livestock: Strategies and lessons.
J. Anim. Sci., 82: 313-328. |
|
|
4. Williams, J.L. (2005) The use of marker-assisted selection
in animal breeding and biotechnology. Rev. Sci. Tech., 24:
379-391.
https://doi.org/10.20506/rst.24.1.1571
PMid:16110903 |
|
|
5. Piper, L.R., Bindon, B.M. and Davis, G.H. (1985) The single
gene inheritance of the high litter size of the Booroola
Merino. In: Land, R.B. and Robinson, D.W., editors. Genetics
of Reproduction in Sheep. Butterworths, London, p115-125.
https://doi.org/10.1016/b978-0-407-00302-6.50016-7 |
|
|
6. Montgomery, G.W., McNatty, K.P. and Davis, G.H. (1992)
Physiology and molecular genetics of mutations that increases
ovulation rate in sheep. Endocr. Rev., 13(2): 309-327.
https://doi.org/10.1210/edrv-13-2-309
PMid:1618165 |
|
|
7. Montgomery, G.W., Crawford, A.M., Penty, J.M., Dodds, K.G.,
Ede, A.J., Henry, H.M., Pierson, C.A., Lord, E.A., Galloway,
S.M., Schmack, A.E., Sise, J.A., Swarbrick, P.A., Hanrahan,
V., Buchanan, F.C. and Hill, D.F. (1993) The ovine Booroola
fecundity gene (FecB) is linked to markers from a region of
human chromosome 4q. Nat. Genet., 4: 410-414.
https://doi.org/10.1038/ng0893-410
PMid:8401591 |
|
|
8. Mulsant, P., Lecerf, F., Fabre, S., Schibler, L., Monget,
P., Lanneluc, I., Pisselet, C., Riquet, J., Monniaux, D.,
Callebaut, I., Cribiu, E., Thimonier, J., Teyssier, J., Bodin,
L., Cognie, Y., Chitour, N. and Elsen, J.M. (2001) Mutation in
the bone morphogenetic protein receptor-IB is associated with
increased ovulation rate in Booroola ewes. Proc. Natl. Acad.
Sci., 98: 5104-5109.
https://doi.org/10.1073/pnas.091577598
PMid:11320249 PMCid:PMC33171 |
|
|
9. Wilson, T., Yang, X.W., Jennifer, L.J., Ian, K.R., Joanne,
M.L., Eric, A.L., Ken, G.D., Grant, A.W., John, C.M., Anne,
R.O., Kenneth, P.M. and Grant, W.M. (2001) Highly prolific
Booroola sheep have a mutation in the intracellular kinase
domain of bone morphogenetic protein IB receptor (ALK-6) that
is expressed in both oocytes and granulosa cells. Biol. Reprod.,
64: 1225-1235.
https://doi.org/10.1095/biolreprod64.4.1225
PMid:11259271 |
|
|
10. Letterio, J.J. and Roberts, A.B. (1998) Regulation of
immune responses by TGF-beta. Annu Rev Immunol., 16: 137-161.
https://doi.org/10.1146/annurev.immunol.16.1.137
PMid:9597127 |
|
|
11. Massague, J. (1998) TGF-beta signal transduction. Annu.
Rev. Biochem., 67: 753-791.
https://doi.org/10.1146/annurev.biochem.67.1.753
PMid:9759503 |
|
|
12. Guan, F., Liu, S.R., Shi, G.Q. and Yang, L.G. (2007)
Polymorphism of FecB gene in nine sheep breeds or strains and
its effects on litter size, lamb growth and development. Anim.
Reprod. Sci. 99: 44-52.
https://doi.org/10.1016/j.anireprosci.2006.04.048
PMid:16859845 |
|
|
13. Hua, G. and Yang, L. (2009) A review of research progress
of FecB gene in Chinese breeds of sheep. Anim. Reprod. Sci.,
116: 1-9.
https://doi.org/10.1016/j.anireprosci.2009.01.001
PMid:19201555 |
|
|
|
14. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989)
Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring
Harbour, Cold Laboratory Press, NY. |
|
|
15. Bassam, B.J., Gustavo, C.A. and Gresshoff, P.M. (1991)
Fast and sensitive silver staining of DNA in polyacrylamide
gels. Anal. Biochem., 196: 80-83.
https://doi.org/10.1016/0003-2697(91)90120-I |
|
|
16. Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J.,
Zhang, Z., Miller, W. and Lipman, D.J. (1997) Gapped BLAST and
PSI-BLAST: A new generation of protein database search
programs. Nucleic Acids Res., 25: 3389-3402.
https://doi.org/10.1093/nar/25.17.3389
PMid:9254694 PMCid:PMC146917 |
|
|
17. Kramer, C.Y. (1957) Extension of multiple range tests to
group correlated adjust means. Biometrics, 13: 13.
https://doi.org/10.2307/3001898 |
|
|
18. Davis, G.H. (2004) Fecundity genes in sheep. Anim. Reprod.
Sci., 82-83: 247-253.
https://doi.org/10.1016/j.anireprosci.2004.04.001
PMid:15271457 |
|
|
19. Davis, G.H., Balakrishnan, L., Ross, I.K., Wilson, T.,
Galloway, S.M., Lumsden, B.M., Hanrahan, J.P., Mullen, M.,
Mao, X.Z., Wang, G.L., Zhao, Z.S., Zeng, Y.Q., Robinson, J.J.,
Mavrogenis, A.P., Papachristoforou, C., Peter, C., Baumung,
R., Cardyn, P., Boujenane, I., Cockett, N.E., Eythorsdottir,
E., Arranz, J.J. and Notter, D.R. (2006) Investigation of the
Booroola (FecB) and Inverdale (FecXI) mutations in 21 prolific
breeds and strains of sheep sampled in 13 countries. Anim.
Reprod. Sci., 92(1-2): 87-96.
https://doi.org/10.1016/j.anireprosci.2005.06.001
PMid:15982834 |
|
|
20. Vacca, G.M., Dhaouadi, A., Rekik, M., Carcangiu, V.,
Pazzola, M. and Dettori, M.L. (2010) Prolificacy genotypes at
BMPR 1B, BMP15 and GDF9 genes in North African sheep breeds.
Small Rumin. Res., 88: 67-71.
https://doi.org/10.1016/j.smallrumres.2009.11.005 |
|
|
21. Silva, B.D., Castro, E.A., Souza, C.J., Paiva, S.R.,
Sartori, R., Franco, M.M., Azevedo, H.C., Silva, T.A., Vieira,
A.M., Neves, J.P. and Melo, E.O. (2011) A new polymorphism in
the growth and differentiation factor 9 (GDF9) gene is
associated with increased ovulation rate and prolificacy in
homozygous sheep. Anim. Genet., 42: 89-92.
https://doi.org/10.1111/j.1365-2052.2010.02078.x
PMid:20528846 |
|
|
22. Wang, Y.Q., Li, Y.X., Zhang, N., Wang, Z.B. and Bai, J.Y.
(2011) Polymorphism of exon 2 of BMP15 gene and its
relationship with litter size of two Chinese goats. Asian Aust.
J. Anim. Sci., 24(7): 905-911.
https://doi.org/10.5713/ajas.2011.10432 |
|
|
23. Moradband, F., Rahimi, G. and Gholizadeh, M. (2011)
Association of polymorphisms in fecundity genes of GDF9, BMP15
and BMP15-1B with litter size in Iranian Baluchi sheep. Asian
Aust. J. Anim. Sci., 24(9): 1179-1183.
https://doi.org/10.5713/ajas.2011.10453 |
|
|
24. Pramod, R.K., Sharma, S.K., Singhi, A., Pan, S. and Mitra,
A. (2013) Differential ovarian morphometry and follicular
expression of BMP15, GDF9 and BMPR1B influence the prolificacy
in goat. Reprod. Domest. Anim., 48(5): 803-809.
https://doi.org/10.1111/rda.12165
PMid:23581245 |
|
|
25. Ahlawat, S., Sharma, R. and Maitra, A. (2013) Screening of
indigenous goats for prolificacy associated DNA markers of
sheep. Gene, 517: 128-131.
https://doi.org/10.1016/j.gene.2012.12.015
PMid:23299026 |
|
|
26. Jia, C.L., Li, N., Zhao, X.B., Zhu, X.P. and Jia, Z.H.
(2005) Association of single nucleotide polymorphisms in exon
6 region of BMPR1B gene with litter size traits in sheep.
Asian Aust. J. Anim. Sci., 18: 1375-1378.
https://doi.org/10.5713/ajas.2005.1375 |
|
|
27. Polley, S., De, S., Brahma, B., Mukherjee, A., Vinesh, P.V.,
Batabyal, S., Arora, J.S., Pan, S., Samanta, A.K., Datta, T.K.
and Goswami, S.L. (2010) Polymorphism of BMPR1B, BMP15 and
GDF9 fecundity genes in prolific Garole sheep. Trop. Anim.
Health Prod., 42(5): 985-993.
https://doi.org/10.1007/s11250-009-9518-1
PMid:20020203 |
|
|
|
28. Sun, W., Chang, H., Musa, H.H. and Chu, M. (2010) Study on
relationship between microsatellite polymorphism and producing
ability on litter size trait of Hu sheep in China. Afr. J.
Biotechnol., 9(50): 8704-8711. |
|
|
29. Demars, J., Fabre, S., Sarry, J., Rossetti, R., Gilbert,
H., Persani, L., Tosser-Klopp, G., Mulsant, P., Nowak, Z.,
Drobik, W., Martyniuk, E. and Bodin, L. (2013) Genome-wide
association studies identify two novel BMP15 mutations
responsible for an atypical hyperprolificacy phenotype in
sheep. PLoS Genet., 9(4): e1003482.
https://doi.org/10.1371/journal.pgen.1003482 |
|
|
30. Feng, T., Geng, C.X., Lang, X.Z., Chu, M.X., Cao, G.L., Di,
R., Fang, L., Chen, H.Q., Liu, X.L. and Li, N. (2011)
Polymorphisms of caprine GDF9 gene and their association with
litter size in Jining Grey goats. Mol. Biol. Rep., 38(8):
5189-5197.
https://doi.org/10.1007/s11033-010-0669-y
PMid:21181498 |
|
|
31. Chu, M.X., Zhao, X.H., Zhang, Y.J., Jin, M., Wang, J.Y.,
Di, R., Cao, G.L., Feng, T., Fang, L. and Ma, Y.H. (2010)
Polymorphisms of BMPR-IB gene and their relationship with
litter size in goats. Mol. Biol. Rep., 37(8): 4033-4039.
https://doi.org/10.1007/s11033-010-0062-x
PMid:20300862 |
|
|
32. Ahlawat, S., Sharma, R., Roy, M., Mandakmale, S., Prakash,
V. and Tantia, M.S. (2016) Genotyping of novel SNPs in BMPR1B,
BMP15, and GDF9 genes for association with prolificacy in
seven Indian goat breeds. Anim. Biotechnol., 27(3): 199-207.
https://doi.org/10.1080/10495398.2016.1167706
PMid:27135147 |
|
|
|
33. Ghaffari, M., Javaremi, A.N. and Rahimi, G. (2009)
Detection of polymorphism in BMPR-IB gene associated with
twining in Shal sheep using PCR-RFLP method. Int. J. Agric.
Biol., 11(1): 97-99. |
|
|
|
34. Borni, J., Sonia, B. and Naouer, D.M. (2011) PCR-RFLP of
BMPR1B gene in North African Barbarine sheep. Res. Opin. Anim.
Vet. Sci., 1(4): 200-203. |
|
|
35. Shafieiyan, Z., Mohammadi, G., Jolodarzadeh, A. and Amiri,
S. (2013) No mutations of FecB and FecGH in Iranian Lory
sheep. Vet. Res. Forum., 4(4): 265-268.
PMid:25568682 PMCid:PMC4279613 |
|
