Open Access
Research
(Published
online: 29-11-2016)
26.
Honeybee product therapeutic as
stem cells homing for ovary failure -
Erma Safitri, Thomas V. Widiyatno
and R. Heru Prasetyo
Veterinary World, 9(11): 1324-1330
doi:
10.14202/vetworld.2016.1324-1330
Erma Safitri :
Department of
Veterinary Reproduction, Faculty of Veterinary Medicine,
Universitas Airlangga, Surabaya, East Java, Indonesia;
Stem Cells
Research Division of Institute Tropical Disease, Universitas
Airlangga, Surabaya, East Java, Indonesia; rma_fispro@yahoo.com
Thomas V. Widiyatno :
Department of
Veterinary Pathology, Faculty of Veterinary Medicine, Universitas
Airlangga, Surabaya, East Java, Indonesia;
th_v_widiyatno@yahoo.co.id
R. Heru Prasetyo :
Department of
Parasitology, Faculty of Medicine, Universitas Airlangga,
Surabaya, East Java, Indonesia; rheru_prasetyo@yahoo.co.id
Received: 09-05-2016, Accepted: 20-10-2016, Published online:
29-11-2016
Corresponding author:
Erma Safitri,
e-mail: rma_fispro@yahoo.com
Citation:
Safitri E, Widiyatno TV, Prasetyo RH (2016) Honeybee product
therapeutic as stem cells homing for ovary failure, Veterinary
World, 9(11): 1324-1330.
Abstract
Aim:
Complexity of
the method of isolation, cultivation in vitro and the
expensive cost of transplantation process of stem cells, it would
require an innovation to homing and differentiation of stem cells
and increase folliculogenesis. The stem cells homing was achieved
through the provision of food or beverages derived from natural
materials like honeybee product. Through honeybee product, there
will be homing of stem cells and accompany with the sources from
the body itself will take place in regeneration of the ovary.
Materials and
Methods:
Female rats model
of degenerative ovary was obtained through food fasting but still
have drinking water for 5 days. It caused malnutrition and damage
of the ovarian tissue. The administration of 50% honeybee product
(T1) was performed for 10 consecutive days, while the positive
control group (T0+) was fasted and not given honeybee product and
the negative control (T0−) not fasted and without honeybee
product. Observations were taken for homing of stem cells, raised
of folliculogenesis, differentiation of stem cells, and
regeneration of the ovarian tissue using routine H&E staining.
Results:
Homing of stem
cells shown the vascular endothelial growth factor and granulocyte
colony-stimulating factor expression; enhancement of
folliculogenesis was indicated by an increase of follicle dee
Graaf count; enhancement of differentiation of stem cells was
indicated by growth differentiation factor-9 expression; and
regeneration of ovarian tissue indicated by intact ovarian tissue
with growing follicles.
Conclusion:
Honeybee product can be induced endogenous stem cells in
regeneration of ovary failure due to malnutrition.
Keywords:
honeybee product, ovary failure, stem cells homing.
References
1. Trounson, A. and McDonald, C. (2015) Stem cell therapies in
clinical trials: Progress and challenges. Cell Stem Cells,
17(1): 11-22.
https://doi.org/10.1016/j.stem.2015.06.007
PMid:26140604 |
|
|
2. Watt, F.M. and Driskell, R.R. (2010) Review: The
therapeutic potential of stem cells. Philos. Trans. R. Soc.,
365: 155-163.
https://doi.org/10.1098/rstb.2009.0149
PMid:20008393 PMCid:PMC2842697 |
|
|
|
3. Safitri, E., Utama, S., Widiyatno, T.V., Sandhika, W. and
Prasetyo, R.H. (2016) Autoregeneration of mice testicle
seminiferous tubules due to malnutrition based on stem cells
mobilization using bee honey. Asian Pac. J. Reprod., 5(1):
30-34. |
|
|
4. Prasetyo, R.H. and Safitri, E. (2016) Effects of honey to
mobilize endogenous stem cells in efforts intestinal and
ovarian tissue regeneration in rats with protein energy
malnutrition. Asian Pac. J. Reprod., 5(3): 198-203.
https://doi.org/10.1016/j.apjr.2016.04.008 |
|
|
5. Caplan, A.I. and Correa, D. (2011) The MSC: An injury
drugstore. Cell Stem Cells, 8: 11-15.
https://doi.org/10.1016/j.stem.2011.06.008
PMid:21726829 PMCid:PMC3144500 |
|
|
6. Volarevic, V., Arsenijevic, N., Lukic, M.L. and Srojkovic,
M. (2011) Concise review: Mesenchymal stem cells treatment of
the complications of diabetes mellitus. Stem cells
regenerative medicine. Stem Cells, 29(1): 5-10.
https://doi.org/10.1002/stem.556 |
|
|
|
7. Halim, D.H., Murty, F., Sandra, A., Boediono, T.,
Djuwantono, B. and Setiawan, B. (2010) Stem Cell Dasar Teori
dan Aplikasi Klinis. 1st ed. Penerbit Erlangga, Jakarta.
p1-10. |
|
|
8. De-Souza, N. (2014) Self-organizing stem cells. Nat.
Methods, 11(31): 29-37.
PMid:24524138 |
|
|
9. Hu, C. and Li, L. (2015) Review: In vitro culture of
isolated primary hepatocytes and stem cell-derived hepatocyte-like
cells for liver regeneration. Protein Cell, 6(8): 562-574.
https://doi.org/10.1007/s13238-015-0180-2
PMid:26088193 PMCid:PMC4506286 |
|
|
10. van-Agthoven, M., Groot, M.T., Verdonck, L.F., Lo-Wenberg,
B., Schattenberg, A.V., Oudshoorn, M., Hagenbeek, A.,
Cornelissen, J.J., Uyl-de-Groot, C.A. and Willemze, R. (2012)
Economic study cost analysis of HLA-identical sibling and
voluntary unrelated allogeneic bone marrow and peripheral
blood stem cell transplantation in adults with acute
myelocytic leukaemia or acute lymphoblastic leukaemia. Bone
Marrow Transplant, 30(4): 243-251.
https://doi.org/10.1038/sj.bmt.1703641
PMid:12203141 |
|
|
|
11. Hozzein, W. (2016) Bee venom accelerates diabetic wound
healing by suppressing the activating transcription factor-3
and inducible nitric oxid synthase-ediated oxidative stress
and by recruiting bone marrow-derived endothelial progenitor
cells in diabetic mice. Proceeding 13th Asian Apicultural
Association Conference. April, 2016. Jeddah, Kingdom of Saudi
Arabia. p134-135. |
|
|
12. Aggarwal, S. and Pittenger, M.F. (2005) Human mesenchymal
stem cells modulate allogeneic immune cell responses. Blood,
10(4): 1815-1822.
https://doi.org/10.1182/blood-2004-04-1559
PMid:15494428 |
|
|
13. Najm, F., Madhavan, M., Zaremba, A., Shick, E., Karl, R.T.,
Factor, D.C., Miller, T.E., Nevin, Z.S., Kantor, C., Sargent,
A., Quick, K.L., Schlatzer, D.M., Tang, H., Papoian, R.,
Brimacombe, K.R., Shen, M.N., Boxer, M.B., Jadhav, A.,
Robinson, A.P., Podojil, J.R., Miller, S.D., Miller, R.H. and
Tesar, P.J. (2015) Drug-based modulation of endogenous stem
cells promotes functional remyelination in vivo. Nature,
522(7555): 216-220.
https://doi.org/10.1038/nature14335
PMid:25896324 PMCid:PMC4528969 |
|
|
|
14. Macey, M.G. (2007) Flow Cytometry, Principle and
Aplications. 1st ed. Human Press, Totowa, NJ. p1-31. |
|
|
|
15. Sakri, F.M. (2012) Madu dan Khasiatnya, Suplemen Sehat
Tanpa Efek Samping. 1st ed. Diandra Pustaka Indonenesia,
Yogyakarta. p20-25. |
|
|
|
16. Nabiuni, M., Azimi, E., Shiravi, A. and Nazari, Z. (2012)
Honey bee venom will differentiate mesenchymal stem cells in
to the osteocyte. International Conference on Applied Life
Sciences, (ICALS 2012), Turkey. September, 10-12. p247-250. |
|
|
17. Kumar, G.L. and Rudbeck, L. (2009) Immunohistochemical
Staining Methods. 5th ed. Dako North America, Carpinteria,
California. p11-14.
PMCid:PMC2630936 |
|
|
18. Caplan, A.I. (2007) Adult mesenchymal stemcells for tissue
engineering versus regenerative medicine. Mini review. J. Cell
Physiol., 213(2): 341-347.
https://doi.org/10.1002/jcp.21200
PMid:17620285 |
|
|
19. Wendy, W.P., Priceb, E.A., Sahooa, D., Beermanc, I.,
Maloneyd, W.J., Rossic, D.J., Schrierb, S.L. and Weissmana,
I.L. (2011) Human bone marrow hematopoietic stem cells are
increased in frequency and myeloid-biased with age. PNAS,
108(50): 20012-20017.
https://doi.org/10.1073/pnas.1116110108
PMid:22123971 PMCid:PMC3250139 |
|
|
20. Santoro, N.F. and Cooper, A.R. (2016) Primary Ovarian
Insufficiency - Clinical Guide to Early Menopause. e-Book. 1st
ed. Springer, Switzerland. p82-83.
https://doi.org/10.1007/978-3-319-22491-6 |
|
|
|
21. Rantam, F.A., Ferdiansyah, M. and Purwati, A. (2014) Stem
Cell Mesenchymal, Hematopoetik dan Model Aplikasi. 2nded.
Airlangga University Press, Surabaya. p45-50, 145-155. |
|
|
22. Eckmann, L. (2006) Animal models of inflammatory bowel
disease, lesson from enteric infections. Ann. N. Y. Acad. Sci.,
1072: 28-38.
https://doi.org/10.1196/annals.1326.008
PMid:17057188 |
|
|
23. Sengupta, P. (2013) The Laboratory rat: Relating its age
with human's. Int. J. Prev. Med., 4(6): 624-630.
PMid:23930179 PMCid:PMC3733029 |
|
|
24. Harrington, A.M., Olteanu, H. and Kroft, S.H. (2012) A
dissection of the CD45/side scatter "Blast Gate". Am. J. Clin.
Pathol., 137(5): 800-804.
https://doi.org/10.1309/AJCPN4G1IZPABRLH
PMid:22523220 |
|
|
25. Palermo, R. (2007) Differential actions of FSH and LH
during folliculogenesis. Reprod. Biomed. Online, 5(3):
326-337.
https://doi.org/10.1016/S1472-6483(10)60347-1 |
|
|
|
26. Rantam, F.A., Ferdiansyah, M. Nasronudin and Purwati, A .
(2009) Stem Cell Exploration. Methods of Isolation and
Culture. 1sted. Airlangga University Press, Surabaya. |
|
|
27. Palermo, R. (2007) Differential actions of FSH and LH
during folliculogenesis. Reprod. Biomed. Online, 5(3):
326-337.
https://doi.org/10.1016/S1472-6483(10)60347-1 |
|
|
28. Barker, N. (2014) Adult intestinal stem cells: Critical
drivers of epithelial homeostasis and regeneration. Nat. Rev.
Mol. Cell Biol., 15(1): 19-33.
https://doi.org/10.1038/nrm3721
PMid:24326621 |
|
|
29. Vander-Flier, L.G. and Clevers, H. (2009) Stem cells,
self-renewal, and differentiation in the intestinal
epithelium. Annu. Rev. Physiol., 71: 241-260.
https://doi.org/10.1146/annurev.physiol.010908.163145
PMid:18808327 |
|
|
30. Hermann, M., Varrier, S. and Mauro, A. (2015) Strategies
to stimulate mobilization and homing of endogenous stem and
progenitor cells for bone tissue repair. Front. Bioeng.
Biotechnol., 2(3): 79.
https://doi.org/10.3389/fbioe.2015.00079 |
|
|
31. Dong, J., Albertini, D.F., Nishimori, K., Kumar, T.J., Lu,
N. and Matzuki, M.M. (1996) Growth differentiation factor-9 is
required during early ovarian folliculogenesis. Nature,
383(6600): 531-535.
https://doi.org/10.1038/383531a0
PMid:8849725 |
|
|
32. Dussaubat, C., Brunet, J.L., Higes, M., Colbourne, J.K.,
Lopez, J., Choi, J.H., Martın-Herna, R., Botias, C., Cousin,
M., McDonnell, C., Bonnet, M., Belzunces, L.P., Moritz, R.F.A.,
Conte, Y.L. and Alaux, C.D. (2012) Gut pathology and responses
to the microsporidium nosemaceranae in the honey bee Apis
mellifera. PLoS One, 7(5): 1-12.
https://doi.org/10.1371/journal.pone.0037017
PMid:22623972 PMCid:PMC3356400 |
|
|
33. Filho, F.L.T., Baracat, E.C., Lee, T.H., Suh, C.S.,
Matsui, M., Chang, J., Shimasaki, S. and Erickson, G.F. (2001)
Abberant expression of growth differentiation factor-9 in
oocytes of polycystic ovary syndrome. J. Clin. Endocrinol.
Metab., 87: 1337-1344.
https://doi.org/10.1210/jcem.87.3.8316
PMid:11889206 |
|
|
34. Lee, H.J., Salesniemi, K., Niikura, Y., Niikura, T.,
Klein, R., Dombkowski, D.M. and Tilly, J.L. (2007) Bone marrow
transplantation generates immature oocytes and rescues
long-term fertility in preclinical mouse model of
chemotherapy-induced premature ovarian failure. J. Clin. Oncol.,
25: 198-204.
https://doi.org/10.1200/JCO.2006.10.3028
PMid:17664466 |
|
|
|
35. Dan, S., Haibo, L. and Hong, L. (2014) Review:
Pathogenesis and stem cell therapy for premature ovarian
failure. OA Stem Cells, 2(1): 1-8. |
|
