Open Access
Research
(Published
online: 30-11-2016)
27.
Amelioration of Gamma-hexachlorocyclohexane
(Lindane) induced renal toxicity by Camellia sinensis in
Wistar rats -
W. L. N. V. Vara Prasad, Ch. Srilatha, N. Sailaja, N. K. B. Raju
and N. Jayasree
Veterinary World, 9(11): 1331-1337
doi:
10.14202/vetworld.2016.1331-1337
W. L. N. V. Vara
Prasad :
Department of
Veterinary Pathology, College of Veterinary Science, Sri
Venkateswara Veterinary University, Tirupati, Andhra Pradesh,
India;
prasad.mudaliyar@gmail.com
Ch. Srilatha :
Department of
Veterinary Pathology, College of Veterinary Science, Sri
Venkateswara Veterinary University, Tirupati, Andhra Pradesh,
India;
ch.srilatha1965@yahoo.co.in
N. Sailaja :
Department of
Veterinary Pathology, College of Veterinary Science, Sri
Venkateswara Veterinary University, Proddatur, Andhra Pradesh,
India;
sailajapath@gmail.com
N. K. B. Raju :
Department of
Veterinary Anatomy, NTR College of Veterinary Science, Sri
Venkateswara Veterinary University, Gannavaram, Andhra Pradesh,
India;
nkbraju@gmail.com
N. Jayasree :
Department of
Veterinary Pathology, College of Veterinary Science, Sri
Venkateswara Veterinary University, Tirupati, Andhra Pradesh,
India; jayasreerdysvvu@gmail.com
Received: 02-08-2016, Accepted: 27-10-2016, Published online:
30-11-2016
Corresponding author:
W. L. N. V. Vara
Prasad, e-mail: prasad.mudaliyar@gmail.com
Citation:
Vara Prasad WLNV, Srilatha Ch, Sailaja N, Raju NKB, Jayasree N
(2016) Amelioration of gamma-hexachlorocyclohexane (lindane)
induced renal toxicity by Camellia sinensis in Wistar rats,
Veterinary World, 9(11): 1331-1337.
Abstract
Aim:
A study to
assess the toxic effects of gamma-hexachlorocyclohexane (γ-HCH) (lindane)
and ameliorative effects of Camellia sinensis on renal
system has been carried out in male Wistar rats.
Materials and
Methods:
Four groups of
rats with 18 each were maintained under standard laboratory
hygienic conditions and provided feed and water ad libitum.
γ-HCH was gavaged at 20 mg/kg b.wt. using olive oil as vehicle to
Groups II. C. sinensis at 100 mg/kg b.wt. was administered
orally in distilled water to Group IV in addition to γ-HCH 20
mg/kg b.wt. up to 45 days to study ameliorative effects. Groups I
and III were treated with distilled water and C. sinensis
(100 mg/kg b.wt.), respectively. Six rats from each group were
sacrificed at fortnight intervals. Serum was collected for
creatinine estimation. The kidney tissues were collected in
chilled phosphate buffer saline for antioxidant profile and in
also 10% buffered formalin for histopathological studies.
Results:
γ-HCH
treatment significantly increased serum creatinine and
significantly reduced the renal antioxidative enzymes catalase,
superoxide dismutase, and glutathione peroxidase. Grossly, severe
congestion was noticed in the kidneys. Microscopically, kidney
revealed glomerular congestion, atrophy, intertubular hemorrhages,
degenerative changes in tubular epithelium with vacuolated
cytoplasm, desquamation of epithelium and urinary cast formation.
A significant reduction in serum creatinine levels, significant
improvement in renal antioxidant enzyme activities and near to
normal histological appearance of kidneys in Group IV indicated
that the green tea ameliorated the effects of γ-HCH, on renal
toxicity.
Conclusion:
This
study suggested that C. sinensis extract combined with γ-HCH
could enhance antioxidant/detoxification system which consequently
reduced the oxidative stress thus potentially reducing γ-HCH
toxicity and tissue damage.
Keywords:
Camellia sinensis,
gamma-hexachlorocyclohexane, kidney.
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