Open Access
Research
(Published
online: 08-11-2016)
6.
Acute phase response in lame crossbred dairy
cattle -
A. Bagga, Swaran Singh Randhawa, S. Sharma and B. K. Bansal
Veterinary World, 9(11): 1204-1208
doi:
10.14202/vetworld.2016.1204-1208
A. Bagga: Department of Animal Husbandry, CVH, Mehatpur,
Jalandhar, Punjab, India; arvindbagga19@gmail.com
Swaran Singh Randhawa: Department of Veterinary Medicine,
College of Veterinary Science, Guru Angad Dev Veterinary and
Animal Sciences University, Ludhiana - 141 004, Punjab, India;
drswaran68@gmail.com
S. Sharma: Department of Veterinary Medicine, College of
Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana - 141 004, Punjab, India; drshukriti@yahoo.co.in
B. K. Bansal: Department of Veterinary Medicine, College of
Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences
University, Ludhiana - 141 004, Punjab, India; baljinderbansal@rediffmail.com
Received: 15-03-2016, Accepted: 16-09-2016, Published online:
08-11-2016
Corresponding author:
Swaran Singh Randhawa, e-mail: drswaran68@gmail.com
Citation:
Bagga A, Randhawa SS, Sharma S, Bansal BK (2016) Acute phase
response in lame crossbred dairy cattle,
Veterinary World, 9(11):
1204-1208.
Abstract
Aim:
The study was undertaken to study acute phase response based on
acute phase proteins (APPs) such as C-reactive protein (CRP),
haptoglobin (Hp), serum amyloid A (SAA), and fibrinogen in lame
crossbred dairy cattle.
Materials and Methods:
Lame animals (n=30) were selected within 3-7 days of being noticed
as lame by the farm veterinarian, from a local dairy farm in
southeast Ludhiana over a period of 6 months, stratified
proportionately with respect to stage of lactation with non-lame
healthy cows (n=10). All the cows were otherwise healthy and did
not have any other inflammatory problems such as pneumonia,
enteritis, mastitis, or any kind of acute uterine inflammation.
Blood samples were collected from all the animals; serum and
plasma samples were separated and stored at −20°C. The levels of
CRP, Hp, and SAA were estimated using Sandwich ELISA, whereas
fibrinogen was estimated by heat precipitation method.
Results:
SAA levels in lame cows were significantly higher (22.19±0.85 μg/ml),
approximately 3 times as compared to non-lame cows (8.89±0.72 μg/ml),
whereas serum Hp concentration was approximately 20 times higher
in the lame cattle (21.71±3.32 mg/dl) as compared to non-lame cows
(1.17±0.07 mg/dl). Fibrinogen also increased in the lame cattle
(3.97±0.22 g/L) as compared to non-lame group (1.40±0.17 g/L).
Serum CRP levels analyzed in the lame cattle for the first time in
the present study, and significant high concentration was
appreciated in lame cattle (4.41±0.33 mg/L) as compared to
non-lame cattle (0.61±0.14 mg/L). Lame cattle were having more of
sole hemorrhages, sole ulcers, and white line lesions as compared
to non-lame cattle.
Conclusion:
It can be concluded that lame cattle exhibit high levels of APPs
including CRP, Hp, SAA, and fibrinogen as compared to non-lame
cattle.
Keywords:
acute phase proteins, cattle, lameness.
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