Veterinary World

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Research (Published online: 08-11-2016)

6. Acute phase response in lame crossbred dairy cattle - A. Bagga, Swaran Singh Randhawa, S. Sharma and B. K. Bansal

Veterinary World, 9(11): 1204-1208



   doi: 10.14202/vetworld.2016.1204-1208



A. Bagga: Department of Animal Husbandry, CVH, Mehatpur, Jalandhar, Punjab, India;

Swaran Singh Randhawa: Department of Veterinary Medicine, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab, India;

S. Sharma: Department of Veterinary Medicine, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab, India;

B. K. Bansal: Department of Veterinary Medicine, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab, India;


Received: 15-03-2016, Accepted: 16-09-2016, Published online: 08-11-2016


Corresponding author: Swaran Singh Randhawa, e-mail:

Citation: Bagga A, Randhawa SS, Sharma S, Bansal BK (2016) Acute phase response in lame crossbred dairy cattle, Veterinary World, 9(11): 1204-1208.

Aim: The study was undertaken to study acute phase response based on acute phase proteins (APPs) such as C-reactive protein (CRP), haptoglobin (Hp), serum amyloid A (SAA), and fibrinogen in lame crossbred dairy cattle.

Materials and Methods: Lame animals (n=30) were selected within 3-7 days of being noticed as lame by the farm veterinarian, from a local dairy farm in southeast Ludhiana over a period of 6 months, stratified proportionately with respect to stage of lactation with non-lame healthy cows (n=10). All the cows were otherwise healthy and did not have any other inflammatory problems such as pneumonia, enteritis, mastitis, or any kind of acute uterine inflammation. Blood samples were collected from all the animals; serum and plasma samples were separated and stored at −20C. The levels of CRP, Hp, and SAA were estimated using Sandwich ELISA, whereas fibrinogen was estimated by heat precipitation method.

Results: SAA levels in lame cows were significantly higher (22.190.85 μg/ml), approximately 3 times as compared to non-lame cows (8.890.72 μg/ml), whereas serum Hp concentration was approximately 20 times higher in the lame cattle (21.713.32 mg/dl) as compared to non-lame cows (1.170.07 mg/dl). Fibrinogen also increased in the lame cattle (3.970.22 g/L) as compared to non-lame group (1.400.17 g/L). Serum CRP levels analyzed in the lame cattle for the first time in the present study, and significant high concentration was appreciated in lame cattle (4.410.33 mg/L) as compared to non-lame cattle (0.610.14 mg/L). Lame cattle were having more of sole hemorrhages, sole ulcers, and white line lesions as compared to non-lame cattle.

Conclusion: It can be concluded that lame cattle exhibit high levels of APPs including CRP, Hp, SAA, and fibrinogen as compared to non-lame cattle.

Keywords: acute phase proteins, cattle, lameness.

1. Prohl, A., Schroedl, W., Rhode, H. and Reinhold, P. (2015) Acute phase proteins as local biomarkers of respiratory infection in calves. BMC Vet. Res., 25(11): 167.
2. Schneider, A. (2015) Acute phase proteins for diagnosis of diseases in dairy cattle. Vet. J., 205: 333-334.
3. Brodzki, P., Kostro, K., Brodzki, A., Wawron, W., Marczuk, J. and Kurek, L. (2015) Inflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum. Theriogenology, 84: 11-18.
4. Brodzki, P., Kostro, K., Brodzki, A. and Ziętek, J. (2015) The concentrations of inflammatory cytokines and acute-phase proteins in the peripheral blood and uterine washings in cows with pyometra. Reprod. Domest. Anim., 50(3): 417-422.
5. Idoate, I., Vander, L.B., Schultz, L. and Heller, M. (2015) Acute phase proteins in naturally occurring respiratory disease of feedlot cattle. Vet. Immunol. Immunopathol., 163: 221-226.
6. Hanthorn, C.J., Dewell, G.A., Dewell, R.D., Cooper, V.L., Wang, C., Plummer, P.J. and Lakritz, J. (2014) Serum concentrations of haptoglobin and haptoglobin-matrix metalloproteinase 9 (Hp-MMP 9) complexes of bovine calves in a bacterial respiratory challenge model. BMC Vet. Res., 10: 285.
PMid:25480520 PMCid:PMC4263037
7. Thomas, F.C., Waterston, M., Hastie, P., Parkin, T., Haining, H. and Eckersall, P.D. (2015) The major acute phase proteins of bovine milk in a commercial dairy herd. BMC Vet. Res., 11: 207.
PMid:26276568 PMCid:PMC4536752
8. Murata, H., Shimada, N. and Yoshioka, M. (2004) Current research on acute phase proteins in veterinary diagnosis: An overview. Vet. J., 168: 28-40.
9. Mackiewicz, A. (1997) Acute phase proteins and transformed cells. Int. Rev. Cytol., 170: 225-300.
10. McDonald, T.L., Larson, M.A., Mack, D.R. and Weber, A. (2001) Elevated extrahepatic expression and secretion of mammary associated serum amyloid A 3 (M-SAA3) into colostrum. Vet. Immunol. Immunopathol., 83: 203-211.
11. Horadagoda, N.U., Knox, K.M.G., Gibbs, H.A., Reid, S.W.J., Horadagoda, A., Edwards, S.E.R. and Eckersall, P.D. (1999) Acute phase proteins in cattle: Discrimination between acute and chronic inflammation. Vet. Rec., 144: 437-441.
12. Jawor, P., Steiner, S., Stefaniak, T., Baumgartner, W. and Rzasa, A. (2008) Determination of selected acute phase proteins during the treatment of limb diseases in dairy cows. Vet. Med., 53: 173-183.
13. Smith, B.I., Kauffold, J. and Sherman, L. (2010) Serum haptoglobin concentrations in dairy cattle with lameness due to claw disorders. Vet. J., 186: 162-165.
14. Nazifi, S., Esmailnezhad, Z., Haghkhah, M., Ghadirian, S. and Mirzaei, A. (2012) Acute phase response in lame cattle with interdigital dermatitis. World J. Microbiol. Biotechnol., 28: 1791-1796.
15. Zahid, U.N., Randhawa, S.S., Hussain, S.A., Randhawa, S.N.S., Mahajan, V. and Kirti, D. (2014) Claw lesions causing clinical lameness in lactating Holstein Frisian crossbred cows. Vet. Med. Int., 2014: 764689.
PMid:25133012 PMCid:PMC4123627
16. Heegaard, P.M., Godson, D.L., Toussaint, M.J., Toornehooj, K., Larsen, L.E., Viuff, B. and Roonsholt, L. (2000) The acute phase response of haptoglobin and serum amyloid A (SAA) in cattle undergoing experimental infection with bovine respiratory syncytial virus. Vet. Immunol. Immunopathol., 77: 151-159.
17. Pyorala, S., Hovinen, M., Simojoki, H., Fitzpatrick, J., Eckersall, P.D. and Orro, T. (2011) Acute phase proteins in milk in naturally acquired bovine mastitis caused by different pathogens. Vet. Rec., 168: 535.
18. Skinner, J.G., Brown, R.A.L. and Roberts, L. (1991) Bovine haptoglobin response in clinically defined field conditions. Vet. Rec., 128: 147-149.
19. Katch, N., Miyamoto, T., Nakagawa, H. and Watanabe, A. (1999) Detection of annexin I and IV and haptoglobin in brochoalveolar lavage fluid from calves experimentally inoculated with Pastuerella hemolytica. Am. J. Vet. Res., 60: 1390-1395.
20. Nazifi, S., Khoshvaghti, A. and Gheisari, H.R. (2008) Evaluation of serum and milk amyloid A in some inflammatory diseases of cattle. Iran. J. Vet. Res., 9: 222-226.
21. Green, L.E., Hedges, V.J., Schukken, Y.H., Blowney, R.W. and Pckington, A.J. (2002) The impact of clinical lameness on the milk yield of dairy cows. J. Dairy Sci., 85: 2250-2256.
22. Hirvonen, J. and Pyorala, S. (1998) Acute-phase response in dairy cows with surgically-treated abdominal disorders. Vet. J., 155: 53-61.
23. Morimatsu, M., Watanabe, A., Yoshimatsu, K., Fujinaga, T., Okubo, M. and Naiki, M. (1991) Elevation of bovine serum C-reactive protein and serum amyloid P component levels by lactation. J. Dairy Res., 58: 257-261.
24. Schrodl, W., Kruger, M., Hien, T.T., Fuldner, M. and Kunze, M. (1995) C-reactive protein as a new parameter of mastitis. Tierarztl. Prax., 23(4): 337-341.