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Research (Published online: 08-11-2016)

7. In vitro evaluation of different varieties of maize fodder for their methane generation potential and digestibility with goat rumen liquor - Shalini Vaswani, Ravindra Kumar, Vinod Kumar, Debashis Roy and Muneendra Kumar

Veterinary World, 9(11): 1209-1213

 

 

   doi: 10.14202/vetworld.2016.1209-1213

 

 

Shalini Vaswani: Department of Animal Nutrition, College of Veterinary Science & Animal Husbandry, U.P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India; shalini_vet@yahoo.com

Ravindra Kumar: Division of Nutrition Feed Resources and Product Technology, Central Institute for Research on Goats, Makhdoom, Farah - 281 122, Uttar Pradesh, India; ravindra.srivastava@gmail.com

Vinod Kumar: Department of Animal Nutrition, College of Veterinary Science & Animal Husbandry, U.P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India; vinodsidhu@rediffmail.com

Debashis Roy: Department of Animal Nutrition, College of Veterinary Science & Animal Husbandry, U.P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India; debashis2k4@gmail.com

Muneendra Kumar: Department of Animal Nutrition, College of Veterinary Science & Animal Husbandry, U.P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura - 281 001, Uttar Pradesh, India; muneendra82@gmail.com

 

Received: 21-04-2016, Accepted: 27-09-2016, Published online: 08-11-2016

 

Corresponding author: Shalini Vaswani, e-mail: shalini_vet@yahoo.com


Citation: Vaswani S, Kumar R, Kumar V, Roy D, Kumar M (2016) In vitro evaluation of different varieties of maize fodder for their methane generation potential and digestibility with goat rumen liquor, Veterinary World, 9(11): 1209-1213.



Aim: To evaluate the methane generation potential and digestibility of different (normal and three high-quality protein maize [HQPM]) varieties of maize fodder with goat rumen liquor in vitro.

Materials and Methods: Methane production potential and digestibility of different varieties of maize fodder were tested in in vitro gas production test. Seven varieties of maize, four normal (HTHM 5101, DHM 117, HM 5, and Shaktiman/900 M Gold), and three high-quality protein (HQPM 5, HQPM 7, and HQPM 9/Vivek) were grown in different plots under the same environmental and agro-climatic conditions. Fodders were harvested at 45-50 days of sowing, and the representative samples of fodder from different varieties of maize were collected for analysis. Dried and grinded form of these maize fodder varieties was tested for gas, methane, and digestibility using goat rumen microflora in in vitro gas syringes.

Results: Gas production (ml/g dry matter [DM]) was highest for HM5 variety (97.66, whereas lowest for HQPM 9 variety (64.22). Gas production (ml/g degraded DM [DDM]) and methane (%) were statistically similar in different varieties of maize fodder. The methane production expressed as ml/g DM and ml/g DDM was significantly (p<0.05) highest for HM 5 (14.22 and 26.62) and lowest for DHM 117 variety (7.47 and 14.13). The in vitro DM digestibility (%) and in vitro organic matter digestibility (%) varied from 47.48 (HQPM 5) to 52.05 (HQPM 9) and 50.03 (HQPM 7) to 54.22 (HM 5), respectively.

Conclusion: The present study concluded that DHM 117 maize variety fodder has lowest methane generation potential and incorporating it in the dietary regime of ruminants may contribute to lower methane production.

Keywords: digestibility and quality protein maize, in vitro, maize varieties, methane.



1. Pal, K., Patra, A.K. and Sahoo, S. (2015) Evaluation of feeds from tropical origin for in vitro methane production potential and rumen fermentation in vitro. Span. J. Agric. Res., 13(3): 1-12.
http://dx.doi.org/10.5424/sjar/2015133-7467
 
2. Wanapat, M., Cherdthong, A., Phesatcha, K. and Kang, S. (2015) Dietary sources and their effects on animal production and environmental sustainability. Anim. Nutr., 1(3): 96-103.
http://dx.doi.org/10.1016/j.aninu.2015.07.004
 
3. Martin, C., Doreau, M. and Morgavi, D.P. (2010) Methane mitigation in ruminants: From microbe to the farm scale. Animal, 4: 351-365.
http://dx.doi.org/10.1017/S1751731109990620
PMid:22443940
 
4. Gerber, P.J., Hristov, A.N., Henderson, B., Makkar, H., Oh, J., Lee, C., Meinen, R., Montes, F., Ott, T., Firkins, J., Rotz, A., Dell, C., Adesogan, A.T., Yang, W.Z., Tricarico, J.M., Kebreab, E., Waghorn, G., Dijkastra, J. and Oosting, S. (2013) Technical options for the mitigation of direct methane and nitrous oxide emissions from livestock: A review. Animal, 7: 220-234.
http://dx.doi.org/10.1017/S1751731113000876
PMid:23739465
 
5. Tubiello, F.N., Salvatore, M., Cóndor, G.R.D., Ferrara, A., Rossi, S., Biancalani, R., Federici, S., Jacobs, H. and Lammini, A. (2014) Agriculture, Forestry and other Land use Emissions by Sources and Removals by Sinks: 1990-2011 Analysis. ESS Working Paper No. 2. FAO, Rome, Italy.
 
6. Patra, A.K. (2012a) Estimation of methane and nitrous oxide emissions from Indian livestock. J. Environ. Monit., 14: 2673-2684.
http://dx.doi.org/10.1039/c2em30396e
PMid:22898933
 
7. Patra, A.K. (2014) Trends and projected estimates of GHG emissions from Indian livestock in comparisons with GHG emissions from world and developing countries. Asian Aust. J. Anim. Sci., 27: 592-599.
http://dx.doi.org/10.5713/ajas.2013.13342
PMid:25049993 PMCid:PMC4093536
 
8. Kim, S.H., Lovelia, L., Mamuad, C., Jeong, Y.J.C., Sung, S.L., Jong, Y.K. and Sang, S.L. (2013) In vitro evaluation of different feeds for their potential to generate methane and change methanogen diversity. Asian Aust. J. Anim. Sci., 26(12): 1698-1707.
http://dx.doi.org/10.5713/ajas.2013.13260
PMid:25049760 PMCid:PMC4092884
 
9. Patra, A.K. (2012b) Enteric methane mitigation technologies for ruminant livestock: A synthesis of current research and future directions. Environ. Monit. Assess., 184: 1929-1952.
http://dx.doi.org/10.1007/s10661-011-2090-y
PMid:21547374
 
10. Kumar, R., Kamra, D.N., Agarwal, N. and Chaudhary, L.C. (2007) In vitro methanogenesis and fermentation of feeds containing oil seed cakes with rumen liquor of buffalo. Asian Aust. J. Anim. Sci., 20: 1196-1200.
http://dx.doi.org/10.5713/ajas.2007.1196
 
11. Agrawal, P.K. and Gupta, H.S. (2010) Enhancement of protein quality of maize using biotechnological options. Anim. Nutr. Feed Technol., 10S: 79-91.
 
12. AOAC. (1995) Official Method of Analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC.
 
13. Menke, K.H. and Steingass, H. (1988) Estimation of the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev., 28: 7-55.
 
14. Van Soest, P.J. and Robertson, J.B. (1988) A Laboratory Manual for Animal Science 612. Cornell University, USA.
 
15. Snedecor, C.W. and Cochran, W.G. (1994) Statistical Method. Iowa State University Press, Ames, Iowa.
 
16. Singh, B., Chaudhary, J.L. and Yadav, C.M. (2011) Effect of feeding different levels of cereal green fodder on the performance of crossbred cows. Anim. Nutr. Feed Technol., 11: 285-292.
 
17. Datt, C., Niranjan, M., Chabra, A., Chattopadhyaya, K. and Dhiman, K.R. (2006) Forage yield, chemical composition and in vitro digestibility of different cultivars of maize (Zea mays L.). Indian J. Dairy Sci., 59: 54-57.
 
18. Tolera, A., Berg, T. and Sundstol, F. (1999) The effect of variety on maize grain and crop residue yield and nutritive value of the stover. Anim. Feed Sci. Technol., 79: 165-177.
http://dx.doi.org/10.1016/S0377-8401(99)00025-5
 
19. Bilal, R.M., Sultan, J.I., Jamili, M. and Nafeesi, M. (2007) Impact of maturity and variety on digestion kinetics of maize (Zea mays). Afr. Crop Sci. Conf. Proc., 8: 589-591.
 
20. Blummel, M., Aiple, K.P., Steingass, H. and Becker, K. (1999) A note on the stoichiometrical relationship of short chain fatty acid production and gas formation in vitro in feedstuffs of widely differing quality. J. Anim. Physiol. Anim. Nutr., 81: 157-167.
http://dx.doi.org/10.1046/j.1439-0396.1999.813205.x
 
21. Getachew, G., Robinson, P.H., De Peters, E.J. and Taylor, S.J. (2004) Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds. Anim. Feed Sci. Technol., 111: 57-71.
http://dx.doi.org/10.1016/S0377-8401(03)00217-7
 
22. Singh, S., Kushwaha, B.P., Nag, S.K., Mishra, A.K., Singh, A. and Anele, U.Y. (2012) In vitro ruminal fermentation, protein and carbohydrate fractionation, methane production and prediction of twelve commonly used Indian green forages. Anim. Feed Sci. Technol., 178: 2-11.
http://dx.doi.org/10.1016/j.anifeedsci.2012.08.019
 
23. Lee, H.J., Lee, S.C., Kim, J.D., Oh, Y.G., Kim, B.K., Kim, C.W. and Kim, K.J. (2003) Methane production potential of feed ingredients as measured by in vitro gas test. Asian Aust. J. Anim. Sci., 16: 1143-1150.
http://dx.doi.org/10.5713/ajas.2003.1143
 
24. Getachew, G., Blummel, M., Makkar, H.P.S. and Becker, K. (1998) In vitro gas measuring techniques for assessment of nutritional quality of feeds: A review. Anim. Feed Sci. Technol., 72: 261-281.
http://dx.doi.org/10.1016/S0377-8401(97)00189-2
 
25. Kirchgessner, M.W. and Muller, H.L. (1994) Methane release from dairy cows and pigs. In: Aguilera, J.F., editor. Proceeding, XIIIth Symposium on Energy Metabolism of Farm Animals. Vol. 76. EAAP CSIC, Spain. p333-348.