Open Access
Research
(Published
online: 22-10-2016)
14.
Detection of Salmonella
species in chicken carcasses using genus specific primer belong
to invA gene in Sohag city, Egypt -
Nahed Mahmoud Abdel-Aziz
Veterinary World, 9(10): 1125-1128
doi:
10.14202/vetworld.2016.1125-1128
Nahed Mahmoud
Abdel-Aziz:
Department of
Food Hygiene, Faculty of Veterinary Medicine, Sohag University,
Naser Street, Sohag, Egypt; Nahedvet2012@yahoo.com
Received: 04-05-2016, Accepted: 18-08-2016, Published online:
22-10-2016
Corresponding author:
Nahed
Mahmoud Abdel-Aziz, e-mail: Nahedvet2012@yahoo.com
Citation:
Abdel-Aziz NM (2016) Detection of Salmonella species in
chicken carcasses using genus specific primer belong to invA
gene in Sohag city, Egypt, Veterinary World, 9(10):
1125-1128.
Abstract
Aim:
This study
aimed to detect Salmonella species found as contaminants
in chicken carcass (thigh, breast, wings, liver, and gizzard).
Materials and
Methods:
A total of 75
chicken samples including thigh, breast, wings, liver, and
gizzard (15 of each) were collected from different markets in
Sohag city for detection of Salmonella species by culture
methods, biochemical tests, serology, and polymerase chain
reaction.
Results:
The
overall incidence of Salmonella contamination of 75
examined samples was found to be 6.6% with the higher percentage
of Salmonella being isolated from liver samples (13.3%)
followed by thigh, wings, gizzard (6.6%) while breast show
negative result.
Conclusion:
Results in this study indicate that contamination of chicken
carcass with Salmonella needs strict hygienic measures to
prevent their transmission to human.
Keywords:
chicken carcass, invA gene, Salmonella spp.
References
|
1. Del Rio, E., Panizo, M., Prieto, M., Alonso-Calleja, C.
and Capita, R. (2007) Effects of various chemical
decontamination on natural flora and sensory characteristics
of poultry. Int. J. Food Microbiol., 115: 268-280. |
|
|
|
2. Mulder, R.W. (1999) Hygiene during transport, slaughter
and processing. In: Richardson, R.I. and Mead, G.C.,
editors. Poultry Meat Science. Poultry Science Symposium
Series. Vol. 25. CABI Publishing, Massachusetts. p277-283. |
|
|
|
3. El-Aziz, D.M.A. (2013) Detection of Salmonella
typhimurium in retail chicken meat and chicken giblets. Asia
Pac. J. Trop. Biomed., 3: 678-681. |
|
|
|
4. Uyttendaele, M.R., Debevere, J.M., Lips, R.M. and Neyts,
K.D. (1998) Prevalence of Salmonella in poultry carcass and
their products in Belgium. Int. J. Food Microbiol., 40: 1-8. |
|
|
|
5. Hernandez, T., Sierra, A., Rodriguez-Alvarez, C., Torres,
A., Arevalo, M.P., Calvo, M. and Arias, A. (2005) Salmonella
enterica serotypes isolated from imported frozen chicken
meat in Canary Islands. J. Food Prot., 68(12): 2702-2706. |
|
|
|
6. Todar, K. (2005) Salmonella and salmonellosis. Todar's
Online Textbook of Bacteriology. © 2005 Kenneth Todar
University of Wisconsin-Madison Department of Bacteriology,
USA. |
|
|
|
7. Li, R., Wang, Y., Shen, J. and Wu, C. (2013) Development
of a novel hexa-plex PCR method for identification and
serotyping of Salmonella species. Foodborne Pathog. Dis.,
11: 75-77. |
|
|
|
8. Chiu, C.H. and Ou, J.T. (1996) Rapid identification of
Salmonella serovars in feces by specific detection of
virulence genes, invA and spvC, by an enrichment broth
culture-multiplex PCR combination assay. J. Clin. Microbiol.,
34: 2619-2622. |
|
|
|
9. Darwin, K.H. and Miller, V.L. (1999) Molecular basis of
the interaction of Salmonella with the intestinal mucosa.
Clin. Microbiol. Rev., 12: 405-428. |
|
|
|
10. Kauffmann, G. (1974) Kauffmann white scheme. J. Acta
Pathol. Microbiol., 61: 385. |
|
|
11. Shanmugasamy, M., Velayutham, T. and Rajeswar, J. (2011)
Inv A gene specific PCR for detection of Salmonella from
broilers. Vet. World, 4(12): 562-564.
http://dx.doi.org/10.5455/vetworld.2011.562-564 |
|
|
|
12. Shah, D., Shringi, S., Besser, T. and Call, D. (2009)
In: Liu, D., editor. Molecular Detection of Foodborne
Pathogens. CRC Press, Taylor & Francis Group. Florida, Boca
Raton, USA. p369-389. |
|
|
|
13. Ruban, S., Thyiageeswaran, M. and Sharadha, R. (2010)
Isolation and identification of Salmonella spp from retail
chickens by polymerase chain reaction. J. Microbiol. Res.,
1(3): 106-109. |
|
|
14. Akbar, A. and Kumar, A. (2013) Prevalence and
antibiogram study of Salmonella and Staphylococcus aureus in
poultry meat. Asia Pac. J. Trop. Biomed., 3(2): 163-168.
http://dx.doi.org/10.1016/S2221-1691(13)60043-X |
|
|
|
15. Karmi, M. (2013) Detection of virulence gene (inva) in
Salmonella isolated from meat and poultry products. Int. J.
Genet., 3(2): 07-12. |
|
|
|
16. Suwit, C., Suvichai, R., Fred, U., Pakpoom, T. and
Prapas, P. (2014) prevalence and antimicrobial resistance of
salmonella isolated from carcasses, processing facilities
and the environment surrounding small scale poultry
slaughterhouses in Thailand. Southeast Asian J. Trop. Med.
Public Health, 45(6): 1392-1400. |
|
|
17. Pedro, H., Waldemir, S.A., Beatriz, D.F., Virginia,
L.A., Dayse, L.A., Dalia, D.P., Elmiro, R.D. and Maria, H.C.
(2016) Prevalence and fluoroquinolones resistance of
Campylobacter and Salmonella isolates from poultry carcasses
in Rio de Janeiro, Brazil. Food Control, 61: 243-247.
http://dx.doi.org/10.1016/j.foodcont.2015.10.002 |
|
|
18. Alali, W., Gaydashov, R., Petrova, E., Panin, A.,
Tugarinov, O., Kulikovskii, A., Mamleeva, D., Walls, I. and
Doyle, M.P. (2012) Prevalence of Salmonella on retail
chicken meat in Russian federation. J. Food Prot., 75(8):
1469-1473.
http://dx.doi.org/10.4315/0362-028X.JFP-12-080
PMid:22856571 |
|
|
19. Jianghui, Z., Yeru, W., Xiaoyu, S., Shenghui, C., Haibin,
X., Baowei, Y., Jinlin, H., Guihua, L., Qian, C., Gang, Z.,
Qiuxia, C. and Fengqin, L. (2014) Prevalence and
quantification of Salmonella contamination in raw chicken
carcasses at the retail in China. J. Food Control, 44:
198-202.
http://dx.doi.org/10.1016/j.foodcont.2014.03.050 |
|
|
|
20. Rodriguez, J., Rondón, I. and Verjan, N. (2014)
Serotypes of Salmonella in broiler carcasses marketed at
Ibague, Colombia. Braz. J. Poult. Sci., 17(4): 545-552. |
|
|
|
21. Jarquin, C., Alvarez, D., Morales, O., Morales, A.J.,
López, B., Donado, P., Valencia, M.F., Arévalo, A., Mu-oz,
F., Walls, I., Doyle, M. and Alali, W. (2015) Salmonella on
raw poultry in retail markets in Guatemala: Levels,
antibiotic susceptibility, and serovar distribution. J. Food
Prot., 9: 162-1769. |
|
|
22. Elgroud, R., Zerdoumi, F., Benazzouz, M.,
Bouzitouna-Bentchouala, C., Granier, S.A., Frémy, S.,
Brisabois, A., Dufour, B. and Millemann, Y. (2009)
Characteristics of Salmonella contamination of broilers and
slaughterhouses in the region of Constantine (Algeria).
Zoonoses Public Health, 56: 84-93.
http://dx.doi.org/10.1111/j.1863-2378.2008.01164.x
PMid:18705656 |
|
|
|
23. Cretu, C., Floristean, V., Carp-Carare, M., Bradatan, G.
and Isan, E. (2009) The Influence of pH and temperature on
Salmonella spp. from fresh, chilled and frozen poultry
carcasses. Cercetări Agronomice în Moldova, XLII(2): 79-84. |
|
|
|
24. Nori, E.M. and Thong, K.L. (2010) Differentiation of
Salmonella enterica based on PCR detection of selected
somatic and flagellar antigen. Afr. J. Microbiol. Res.,
4(9): 871-879. |
|
|
|
25. Jennifer, D., Boddicker, B., Knosp, M. and Bradley, D.J.
(2003) Transcription of the Salmonella invasion gene
activator, hilA requires hilD Activation in absence of
negative regulators. J. Bacteriol., 185(2): 525-533. |
|
|
26. Ibrahim, W., Abd El-Ghany, W., Nasef, S. and Hatem, M.
(2014) A comparative study on the use of real time
polymerase chain reaction (RT-PCR) and standard isolation
techniques for the detection of Salmonella in broiler
chicks. Int. J. Vet. Sci. Med., 2(1): 67-71.
http://dx.doi.org/10.1016/j.ijvsm.2013.11.001 |
|
|
|
27. Herikstad, H., Motarjemi, Y. and Tauxe, R.V. (2002)
Salmonella surveillance: Aglobal survey of public health
serotyping. Epidemiol. Infect., 129: 1-8. |
|
