Veterinary World

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Research (Published online: 26-10-2016)

18. Prevalence of Campylobacter jejuni and Campylobacter coli in raw milk and some dairy products - Mona A. El-Zamkan and Karima G. Abdel Hameed

Veterinary World, 9(10): 1147-1151



   doi: 10.14202/vetworld.2016.1147-1151



Mona A. El-Zamkan: Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt;

Karima G. Abdel Hameed: Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt;


Received: 15-05-2016, Accepted: 10-09-2016, Published online: 26-10-2016


Corresponding author: Mona A. El-Zamkan, e-mail:

Citation: El-Zamkan MA, Abdel Hameed KG (2016) Prevalence of Campylobacter jejuni and Campylobacter coli in raw milk and some dairy products, Veterinary World, 9(10): 1147-1151.

Aim: This study was accomplished to test raw milk and certain dairy products sold in local markets of Qena, Egypt, for the presence of Campylobacter coli and Campylobacter jejuni.

Materials and Methods: A total of 150 samples of raw milk, kareish cheese, and yoghurt (50 samples each) were subjected first to enrichment in Bolton broth at 42°C for 2 days under a microaerobic condition, subsequently campylobacter blood free selective agar plates were cultured and incubated in the same condition of the broth. Based on the morphological and biochemical themes of the growing colonies, it was further classified into Campylobacter spp. The identified isolates were later affirmed by polymerase chain reaction using primers that were designed to locate hipO genes in C. jejuni and glyA in C. coli.

Results: Of the total 150 examined samples of raw milk and soft cheese samples; 37 (24.6%) samples were contaminated with Campylobacter spp. C. jejuni was dominating in this study in 20%, 14%, and 8% of the examined raw milk, kareish cheese, and yoghurt samples, respectively. No sample harbored C. coli.

Conclusion: Campylobacter spp. could be detected in 24.6% of the investigated samples. C. jejuni isolated from 14% of the total tested samples, while C. coli could not be detected from the examined samples. Campylobacter spp. is rampant in the areas of poor hygienic conditions making products made from raw milk of public health hazard.

Keywords: Campylobacter coli, Campylobacter jejuni, dairy products, multiplex polymerase chain reaction, raw milk.

1. Platts-Mills, J.A. and Kosek, M. (2014) Update on the burden of Campylobacter in developing countries. Curr. Opin. Infect. Dis., 27(5): 444-450.
PMid:25023741 PMCid:PMC4542018
2. Wei, B., Cha, S.Y., Yoon, R.H., Kang, M., Roh, J.H., Seo, H.S., Lee, J.A. and Jang, H.K. (2016) Prevalence and antimicrobial resistance of Campylobacter spp. isolated from retail chicken and duck meat in South Korea. Food Control, 62: 63-68.
3. Murray, C.J., Vos, T., Lozano, R., et al. (2012) Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990-2010: A systematic analysis for the Global Burden of Disease Study 2010. Lancet, 380: 2197-2223.
4. Gharst, G., Oyarzabal, O.A. and Hussain, S.K. (2013) Review of current methodologies to isolate and identify Campylobacter spp. From foods. J. Microbiol. Methods, 95: 84-92.
5. Vencia, W., Nogarol, C., Bianchi, D.M., Gallina, S., Zuccon, F., Adriano, A., Gramaglia, M. and Decastelli, L. (2014) Validation according to ISO 16140:2003 of a commercial real-time PCR-based for detecting Campylobacter jejuni, C. coli, and C. lari in foods. Int. J. Food Microbiol., 177: 78-80.
6. Blaser, M.J. and Engberg, J. (2008) Clinical aspects of Campylobacter jejuni and Campylobacter coli infections. In: Nachamkin, I., Szymanski, C.M., Blaser, M.J., editors. Campylobacter. ASM Press, Washington, DC. p99-121.
7. Smith, J. (2002) Campylobacter jejuni infection during pregnancy: Long-term consequences of associated bacteremia, Guillain-Barre syndrome, and reactive arthritis. J. Food Prot., 65(4): 696-708.
8. Connor, B.A. and Riddle, M.S. (2013) Review post-infectious sequelae of travelers' Diarrhea. J. Travel Med., 20: 303-312.
9. Ganan, M., Silvan, J.M., Carrascosa, A.V. and Martinez-Rodriguez, A.J. (2012) Review, alternative strategies to use antibiotics or chemical products for controlling Campylobacter in the food chain. Food Control, 24: 6-14.
10. Coker, K.O., Isokpehi, R.D., Bolaji, N., Thomas, B.N., Amisu, K.O. and Obit, C.L. (2002) Human campylobacteriosis in developing countries. Emerg. Infect. Dis., 8: 237-243.
PMid:11927019 PMCid:PMC2732465
11. Hussain, I., Mahmood, M.S., Akhtar, M. and Khan, A. (2007) Prevalence of Campylobacter species in meat, milk and other food commodities in Pakistan. J. Food Microbiol., 24: 219-222.
12. Painter, J.A., Hoekstra, R.M., Ayers, T., Tauxe, R.V., Braden, C.R., Angulo, F.J. and Griffin, P.A. (2013) Attribution of foodborne illnesses, hospitalizations, and deaths to food commodities by using outbreak data, United States, 1998-2008. Emerg. Infect. Dis., 19: 407-415.
PMid:23622497 PMCid:PMC3647642
13. Taylor, E.V., Herman, K.M., Ailes, E.C., Fitzgerald, C., Yoder, J.S., Mahon, B.E. and Tauxe, R.V. (2013) Common source outbreaks of Campylobacter infection in the USA. Epidemiol. Infect., 141: 987-996.
14. FDA. (2001) Campylobacter. Ch. 7. In: Bacteriological Analytical Manual. Available from: Last accessed on 21-08-2016.
15. Wang, G., Clark, C.G., Taylor, T.M., Pucknell, C., Barton, C., Price, L., Woodward, D.L. and Rodgers, F.G. (2002) Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. Fetus. J. Clin. Microbiol., 40: 4744-4747.
PMid:12454184 PMCid:PMC154608
16. Nyati, K.K. and Nyati, R. (2013) Role of Campylobacter jejuni infection in the pathogenesis of Guillain-Barre syndrome: An update. Biomed. Res. Int., 2013: 852195.
PMid:24000328 PMCid:PMC3755430
17. Yang, C., Jiang, Y., Huang, K., Zhu, C. and Yin, Y. (2003) Application of real-time PCR for quantitative detection of Campylobacter jejuni in poultry, milk and environmental water. FEMS Immunol. Med. Microbiol., 38: 265-271.
18. Callon, C., Gilbert, F.B., Cremoux, R.D. and Montel, M.C. (2008) Application of variable numbers of tandem repeat analysis to determine the origin of S. aureus contamination from milk to cheese in goat cheese farms. Food Control, 19: 143-150.
19. Barakat, A.M.A., Mona, M.S., El Fadaly, H.A.A., Nagwa, S.R., Nashwa, O.K., Eman, R.H., Kotb, M.H.R., Zeinab, M.S., Girh, A., Dalia, M.S. and Mona, S.Z. (2015) Zoonotic hazards of campylobacteriosis in some areas in Egypt. Life Sci. J., 12(7): 9-14.
20. Muehlherr, J.E., Zweifel, C., Corti, S., Blanco, J.E. and Stephan, R. (2011) Microbiological quality of raw bulk-tank milk in Switzerland. J. Dairy Sci., 86(12): 3849-3856.
21. Haghi, F., Zeighami, H., Naderi, G., Samei, A., Roudashti, S., Bahari, S. and Shirmast, P. (2015) Detection of major food-borne pathogens in raw milk samples from dairy bovine and ovine herds in Iran. Small Rumin. Res., 131: 136-140.
22. Gergs, A.E. (2004) The Zoonotic Importance of Campylobacter Infection in Man and Animals. Thesis, (M. Sc). Faculty of Veterinary Medicine, Assiut University, Egypt.
23. Robison, R.K. (1990) Dairy Microbiology. 2nd ed. Chapman and Hall, London, New York.
24. André, M.C.D., Campos, M.R.H., Borges, L.J., Kipnis, A., Pimenta, F.C. and Serafini, A.I.B. (2008) Comparison of Staphylococcus aureus isolates from food handlers, raw bovine milk and minas frescal cheese by antibiogram and pulsed field gel electrophoresis following SmaI digestion. Food Control, 19: 200-207.
25. El-Sharoud, W.M. (2009) Prevalence and survival of Campylobacter in Egyptian dairy products. Food Res. Int. J., 42(55-6): 622-626.
26. Mina, H. and Thanaa, N. (2004) Occurrence of Campylobacter species in milk and some milk products. Thesis, (M.V.Sc). Faculty of Veterinary Medicine, Assiut University, Egypt.
27. Aygun, O. and Pehlivanlar, S. (2006) Campylobacter and Listeria species in the raw milk and dairy products in Antakya, Tyrkey. Food Control, 17(8): 676-679.
28. Epps, S.V.R., Harvey, R.B., Hume, M.E., Phillips, T.D., Anderson, R.C. and Nisbet, D.J. (2013) Foodborne Campylobacter: Infections, metabolism, pathogenesis and reservoirs. Int. J. Environ. Res. Public Health, 10: 6292-6304.
PMid:24287853 PMCid:PMC3881114
29. Nakari, U.M.A., Puhakka, A. and Siitonen, A. (2008) Correct identification and discrimination between Campylobacter jejuni and C. coli by a standardized hippurate test and species-specific polymerase chain reaction. Eur. J. Clin. Microbiol. Infect. Dis., 27: 513-518.