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Research (Published online: 28-10-2016)

20. Evaluation of salivary electrolytes during estrous cycle in Murrah buffaloes with reference to estrus detection - Indu Devi, Pawan Singh, Surerder Singh Lathwal, A. Kumaresan and Kuldeep Dudi

Veterinary World, 9(10): 1157-1161

 

 

   doi: 10.14202/vetworld.2016.1157-1161

 

 

Indu Devi: Livestock Production Management Section, ICAR - National Dairy Research Institute, Karnal - 132 001, Haryana, India; indulathwal@gmail.com

Pawan Singh: Livestock Production Management Section, ICAR - National Dairy Research Institute, Karnal - 132 001, Haryana, India; pawansinghdabas@gmail.com

Surerder Singh Lathwal: Livestock Production Management Section, ICAR - National Dairy Research Institute, Karnal - 132 001, Haryana, India; lathwal314@gmail.com

A. Kumaresan: Department of Animal Reproduction (Livestock Production Management), Livestock Research Centre, ICAR - National Dairy Research Institute, Karnal - 132 001, Haryana, India; ogkumaresan@gmail.com

Kuldeep Dudi: Animal Nutrition Group, National Dairy Development Board, SCF-80, Panchkula - 134 109, Haryana, India; dr.dudi.kuldeep@gmail.com

 

Received: 04-06-2016, Accepted: 14-09-2016, Published Online: 28-10-2016

 

Corresponding author: Indu Devi, e-mail: indulathwal@gmail.com


Citation: Devi I, Singh P, Lathwal SS, Kumaresan A, Dudi K (2016) Evaluation of salivary electrolytes during estrous cycle in Murrah buffaloes with reference to estrus detection, Veterinary World, 9(10): 1157-1161.



Aim: Timely estrus detection is one of the critical factors for increasing reproductive efficiency in buffaloes. In recent decades, saliva has become a more popular as a noninvasive source for determining physiological status of animals by various biochemical electrolytes. This study was designed to assess and correlate changes in different salivary minerals concentration (calcium, inorganic phosphorus, magnesium, sodium, potassium, and chloride) during different stages of the estrous cycle in Murrah buffaloes.

Materials and Methods: The saliva samples were collected during the different phases of the estrous cycle from 20 Murrah buffaloes in early morning hours and were assayed using respective minerals assay kits.

Results: The concentrations of calcium (8.76±0.08-12.11±0.11 mg/dl), inorganic phosphorus (6.56±0.13-14.72±4.50 mg/dl), magnesium (2.27±0.14-5.79±0.15 mg/dl), sodium (139.47±0.31-159.62±1.22 mmol/L), potassium (12.40±0.22-26.85±1.22 mmol/L), and chloride (109.28±0.41-137.07±0.68 mmol/L) varied during the different phases of estrous cycle. The concentration of calcium, inorganic phosphorus, magnesium, sodium, potassium, and chloride in saliva were significantly (p<0.01) higher during estrus phase compared to other phases of the estrous cycle. All these minerals were positively and significantly (p<0.0001) related to estrogen concentration while salivary concentrations of calcium, magnesium, sodium, and chloride showed a significant (p<0.0001) negative correlation with progesterone level in blood plasma.

Conclusion: These preliminary findings indicate that there are definite variations in salivary mineral and electrolyte concentrations during different phases of the estrous cycle. These results may be used as an aid for estrus detection/ confirmation in buffaloes although validation of the results using a large number of animals is required.

Keywords: heat detection, noninvasive method, saliva electrolytes, silent heat.



1. Srivastava, A.K. and Kumaresan, A. (2014) Scope of buffaloes in Indian dairy industry. Asian Buffalo Mag., 1: 16-27.
 
2. Bulletin of World Dairy Situation. (2013). Available from: www.ukidf.org/documents/WorldDairySituation2013.pdf. Last accessed on 12-10-2016.
 
3. Available from: www.icar.org.in/Vision%202050%20NRCM,%20Hyderabad.pdf. Last accessed on 13-10-2016
 
4. Department of Animal Husbandry, Dairying and Fisheries (2012). Government of India, 19th Livestock Census. Available from: dahd.nic.in/sites/default/files/19%20th%20Livestock%20%202012.pdf. Last accessed on 12-10-2016.
 
5. Thakur, K.S.R., Kumar, N., Kumar, P., Chaurasia, S. and Patel, N.B. (2013) Heat detection techniques in cattle and buffalo. Vet. World, 6(6): 363-369.
 
6. Dadlani, A.G., Chandwani, S., Desai, C.A. and Pandya, K.D. (1982) Serum electrolytes during various phases of menstrual cycle. Indian J. Phys. Pharmacol., 26: 302-306.
PMid:7152639
 
7. Brommage, R., Binacua, C. and Carrie, A.L. (1993) Ovulation associated increase in intestinal calcium absorption during the rat estrous cycle is blunted by ovariectomy. Biol. Reprod., 49(3): 544-548.
http://dx.doi.org/10.1095/biolreprod49.3.544
 
8. Alagendran, S., Archunan, G. and Achiraman, S. (2007) Prediction of ovulation in women through the occurrence of salivary fern prototype. ICFAI J. Life Sci., 1: 7-15.
 
9. Landau, R.L. and Lugibih, K. (1958) Inhibition of the sodium-retaining influence of aldosterone by progesterone. J. Clin. Endocr. Metab., 18: 1237-1245.
http://dx.doi.org/10.1210/jcem-18-11-1237
PMid:13587641
 
10. Patricia, A.D. (1987) Magnesium and zinc status during menstrual cycle. Am. J. Obstet. Gynecol., 157: 964-968.
http://dx.doi.org/10.1016/S0002-9378(87)80096-0
 
11. Ali, F., Lodhi, L.A., Qureshi, Z.I., Samad, H.A. and Shahid, R.U. (2004) Some serum biochemical constituents of mares during different phases of reproductive cycle. Pak. Vet. J., 24(3): 147-152.
 
12. Pitkin, R.M., Reynolds, W.A., Williams, G.A. and Hargis, G.K. (1978) Calcium-regulating hormones during the menstrual cycle. J. Clin. Endocr. Metab., 47: 626-632.
http://dx.doi.org/10.1210/jcem-47-3-626
PMid:263315
 
13. Silberberg, M. and Silberberg, R. (1956) Steroid hormones and bone. In: Bourne, G.H., editor. Biochemistry and Physiology of Bone. Academic Press, New York. p632-644.
 
14. Johnson, A.J., James, J.O., Baumber, J.S. and Schneider, E.G. (1970) Effect of estrogen and progesterone on electrolyte balance in normal dog. Am. J. Physiol., 219: 1691-1697.
PMid:5485687
 
15. Mikkelsen, W.M., Dodge, H.J., Valkenburg, H. and Himes, S. (1965) The distribution of serum uric acid values in a population unselected as to gout and hyperuricaemia. Am. J. Med., 39: 242-251.
http://dx.doi.org/10.1016/0002-9343(65)90048-3
 
16. Alagendran, S., Devi, C.A., Karthikeyan, K., Arulmozhi, N. and Pushpa, N. (2009) Evaluation of thyroid profile in human saliva with special reference to ovulation. Res. J. Med. Sci., 4: 441-445.
 
17. Pattanasuttinont, S., Sereepapong, W. and Suwajanakorn, S. (2007) The salivary ferning test and ovulation in clomiphene citrate-stimulated cycles. Med. Assoc. Thai., 90: 876-883.
 
18. Oster, G. and Yang, S.L. (1972) Cyclic variation of sialic acid content in saliva. Am. J. Obstet. Gynecol., 114: 190-193.
http://dx.doi.org/10.1016/0002-9378(72)90056-7
 
19. Yilmaz, O., Yazici, E., Kahraman, A., Ozenc, E. and Ucar, M. (2014) The relationship between ovarian follicle population and follicle size during different stages of estrous cycle in Anatolian Water buffaloes (Bubalus bubalis). Rev. Méd. Vét., 165(3-4): 111-115.
 
20. Mondal, S., Suresh, K.P. and Nandi, S. (2010) Endocrine profile of oestrous cycle in buffaloes: A meta-analysis. Asian Aust. J. Anim., 23(2): 169-174.
http://dx.doi.org/10.5713/ajas.2010.90193
 
21. Pahwa, G.S. and Pandey, R.S. (1983) Gonadal steroid hormone concentration in blood plasma and milk of primiparous and multiparous pregnant and non pregnant buffaloes. Theriogenology, 19: 491-505.
http://dx.doi.org/10.1016/0093-691X(83)90170-X
 
22. Rao, T.K.S., Kumar, N., Kumar, P., Chaurasia, S. and Patel, N.B. (2013) Heat detection techniques in cattle and buffalo. Vet. World, 6(6): 363-369.
http://dx.doi.org/10.5455/vetworld.2013.363-369
 
23. Suthar, V.S. and Dhami, A.J. (2010) Estrus detection methods in Buffalo. Vet. World, 3(2): 93-96.