Veterinary World

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Research (Published online: 30-10-2016)

21. Seroepidemiological survey of Neospora caninum and its risk factors in farm dogs in Nakuru district, Kenya - Tequiero Abuom Okumu, John Njenga Munene, James Wabacha, Victor Tsuma and John Van Leeuwen

Veterinary World, 9(10): 1162-1166



   doi: 10.14202/vetworld.2016.1162-1166



Tequiero Abuom Okumu: Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, Nairobi City, Kenya;

John Njenga Munene: Department of Clinical Studies, Faculty of Veterinary Medicine and Surgery, Egerton University, Njoro, Nakuru County, Kenya;

James Wabacha: Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, Nairobi City, Kenya; African Union Inter African Bureau for Animal Resources, Nairobi, Kenya;

Victor Tsuma: Department of Clinical Studies, Faculty of Veterinary Medicine, University of Nairobi, Nairobi City, Kenya;

John Van Leeuwen: Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Canada;


Received: 20-04-2016, Accepted: 15-09-2016, Published online: 30-10-2016


Corresponding author: Tequiero Abuom Okumu, e-mail:

Citation: Okumu TA, Munene JN, Wabacha J, Tsuma V, Van Leeuwen J (2016) Seroepidemiological survey of Neospora caninum and its risk factors in farm dogs in Nakuru district, Kenya, Veterinary World, 9(10): 1162-1166.

Aim: The objective of this study was to determine the seroprevalence of Neospora caninum (NC) and its risk factors in farm dogs in Kenya.

Materials and Methods: As part of a longitudinal study on dairy cattle abortion in 2010 in Kenya, serum samples were collected from 84 dogs in 53 randomly selected dairy cattle farms to determine the seroprevalence and risk factors of seropositivity for NC.

Results: 15 (17.9%) of the dogs were seropositive to NC antibodies, and at least one seropositive dog was found in 12 (22.6%) of the 53 farms. The final multivariable logistic regression model identified free-roaming as the only factor significantly associated with seropositivity (odds ratio=4.48; p=0.03).

Conclusion: The findings of this study indicate that canine neosporosis does exist in Kenya and that farmers should restrict their dogs from roaming to reduce the risk of their dogs becoming a reservoir for NC. More studies need to be carried out to determine the reproductive effects of NC on dairy cattle in Kenya.

Keywords: cross-sectional study, dogs, Kenyan dairy farms, Neosporosis.

1. Dubey, J.P. (1999) Recent advances in Neospora and neosporosis. Vet. Parasitol., 84: 349-367.
2. Silva, D., Lobato, J., Mineo, T. and Mineo, J. (2007) Evaluation of serological tests for the diagnosis of Neospora caninum infection in dogs: Optimization of cut offtitres and inhibition studies of cross-reactivity with Toxoplasma gondii. Vet. Parasitol., 143: 234-244.
3. Gondim, L.F., McAllister, M.M., Anderson-Sprecher, R.C., Björkman, C., Lock, T.F., Firkins, L.D., Gao, L. and Fischer, W.R. (2004)Transplacental transmission and abortion in cows administered Neospora caninum oocysts. J. Parasitol., 90:1394-1400.
4. Gondim, L.F. (2006) Neospora caninum in wildlife. Trends Parasitol., 22: 247-252.
5. Steinman, A., Shpigel, N.Y., Mazar, S., King, R., Baneth, G., Savitsky, I. and Shkap, V. (2006) Low seroprevalence of antibodies to Neospora caninum in wild canids in Israel. Vet. Parasitol., 137: 155-158.
6. Darwich, L., Cabezón, O., Echeverria, I., Pabón, M., Marco, I., Molina-López, R., Alarcia-Alejos, O., López-Gatius, F., Lavín, S. and Almería, S. (2012) Presence of Toxoplasma gondii and Neospora caninum DNA in the brain of wild birds. Vet. Parasitol., 183: 377-381.
7. Lopez-Sicupira, P.M., de Magalhăes, V.C., Galvăo, G.S., Pereira, M.J., Gondim, L.F. and Munhoz, A.D. (2012) Factors associated with infection by Neospora caninum in dogs in Brazil. Vet. Parasitol., 185: 305-308.
8. Paradies, P.,Capelli, G., Testini, G., Cantacessi, C., Trees, A.J. and Otranto, D. (2007) Risk factors for canine neosporosis in farm and kennel dogs in Southern Italy. Vet. Parasitol., 145: 240-244.
9. Dubey, J.P. and Lindsay, D.S. (1996) A review of Neospora caninum and neosporosis. Vet. Parasitol., 67: 1-59.
10. Dubey, J.P. (2005) Neosporosis in cattle. Vet. Clin. N. Am. Food Anim. Pract., 21:473-483.
11. Yang, N., Cui, X., Qian, W., Yu, S. and Liu, Q. (2012) Survey of nine abortifacient infectious agents in aborted bovine foetuses from dairy farms in Beijing, China, by PCR. Acta Vet. Hung., 60: 83-92.
12. Wilson, D.J., Orsel, K., Waddington, J., Rajeev, M., Sweeny, A.R., Joseph, T., Grigg, M.E. and Raverty, S.A. (2016) Neospora caninum is the leading cause of bovine foetal loss in British Columbia, Canada. Vet. Parasitol., 218: 46-51.
13. Reichel, M.P., Ayanegui-Alcérreca, A.M., Gondim, L.F. and Ellis, J.T. (2013) What is the global economic impact of Neospora caninum in cattle - The billion dollar question. Int. J. Parasit., 43:133-142.
14. Barber, J.S., Gasser, R.B., Ellis, J., Reichel, M.P., McMillan, D. and Trees, J. (1997) Prevalence of antibodies to Neospora caninum in different canid populations. J. Parasitol., 83: 1056-1058.
15. Cruz-Vázquez, C., Medina-Esparza, L., Marentes, A., Morales-Salinas, E. and Garcia-Vázquez, Z. (2008) Seroepidemiological study of Neospora caninum infection in dogs found in dairy farms and urban areas of Aguascalientes, Mexico. Vet. Parasitol., 157:139-143.
16. Hornok, S.,Edelhofer, R. and Hajtós, I. (2006) Seroprevalence of neosporosis in beef and dairy cattle herds in Northeast Hungary. Acta Vet. Hung., 54: 485-491.
17. Yang, Y., Zhang, Q., Kong, Y., Ying, Y., Kwok, O.C., Liang, H. and Dubey, J.P. (2014) Low prevalence of Neospora caninum and Toxoplasma gondii antibodies in dogs in Jilin, Henan and Anhui provinces of the People's Republic of China. BMC Vet. Res., 10: 295-301.
PMid:25495616 PMCid:PMC4272784
18. Wu, J.T., Dreger, S., Chow, E.Y. and Bowlby, E.E. (2002) Validation of commercial Neospora caninum antibody enzyme linked immunosorbent assays. Can. J. Vet. Res., 66: 264-271.
PMid:12418782 PMCid:PMC227014
19. Robbe, D., Passarelli, A., Gloria, A., Di Cesare, A., Capelli, G., Iorio, R. and Traversa, D. (2016) Neospora caninum seropositivity and reproductive risk factors in dogs. Exp. Parasitol., 164: 31-35.
20. Maia, A., Cortes, H., Brancal, H., Lopes, A.P., Pirenta, P., Campino, L. and Cardoso, L. (2014) Prevalence and correlates of antibodies of Neospora caninum in dogs in Portugal. Parasite, 29: 1-4.
21. Nazir, M.M., Maqbool, A., Akhtar, M., Ayaz, M., Ahmad, A.N., Ashraf, K., Ali, A., Alam, M.A., Ali, M.A., Khalid, A.R. and Lindsay, D.S. (2014) Neospora caninum prevalence in dogs raised under different living conditions. Vet. Parasitol., 204: 364-368.
22. Mitrea, I.L., Enachescu, V. and Ionita, M. (2013) Neospora caninum infection in dogs from Southern Romania: Coproparasitological study and serological follow-up. J. Parasitol., 99: 365-377.
23. Costa, K.S., Uzeda, R.S., Pinheiro, A.M., Almeida, M.A.O., Arau'jo, F.R., McAllister, M.M. and Gondim, L.F.P. (2008) Chickens (Gallus domesticus) are natural intermediate hosts of Neospora caninum infections. Int. J. Parasitol., 38: 157-159.
24. Silva, R.C. and Machado, G.P. (2016) Canine neosporosis: Perspectives on pathogenesis and management. Vet. Med. Res. Rep., 7: 59-70.
25. Dohoo, I., Martin, W. and Stryhn, H. (2009) Veterinary Epidemiologic Research. VER Inc., Berkley Way, Charlottetown, Prince Edward Island. 19.