Veterinary World

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Research (Published online: 10-10-2016)

5. Molecular identification of Mycoplasma synoviae from seroprevalent commercial breeder farms at Chittagong district, Bangladesh - Md. Inkeyas Uddin, Md. Harisul Abid, Md. Shafiqul Islam, Tofazzal Md. Rakib, Ashim Baran Sen, Shah Mohammed Ziqrul Haq Chowdhury, Md. Nurul Anwar and Kazi Md. Kamaruddin

Veterinary World, 9(10): 1063-1069



   doi: 10.14202/vetworld.2016.1063-1069



Md. Inkeyas Uddin: Poultry Research and Training Centre, Chittagong Veterinary and Animal Sciences University, Khulshi - 4225, Chittagong, Bangladesh;

Md. Harisul Abid: Department of Livestock Services, People’s Republic of Bangladesh;

Md. Shafiqul Islam: Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University, Khulshi - 4225, Chittagong, Bangladesh;

Tofazzal Md. Rakib: Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University, Khulshi - 4225, Chittagong, Bangladesh;

Ashim Baran Sen: Department of Livestock Services, People’s Republic of Bangladesh;

Shah Mohammed Ziqrul Haq Chowdhury: Livestock Division, Bangladesh Agricultural Research Council, Dhaka - 1202, Bangladesh;

Md. Nurul Anwar: Port City International University, Chittagong, Bangladesh;

Kazi Md. Kamaruddin: Livestock Division, Bangladesh Agricultural Research Council, Dhaka - 1202, Bangladesh;


Received: 12-01-2016, Accepted: 26-08-2016, Published online: 10-10-2016


Corresponding author: Mohammed Inkeyas Uddin, e-mail:

Citation: Uddin MI, Abid MH, Islam MS, Rakib TM, Sen AB, Chowdhury SMZH, Anwar MN, Kamaruddin KM (2016) Molecular identification of Mycoplasma synoviae from seroprevalent commercial breeder farms at Chittagong district, Bangladesh, Veterinary World, 9(10): 1063-1069.

Aim: Worldwide, Mycoplasma synoviae (MS) is an important pathogen of poultry, especially for chicken and turkey. It causes respiratory tract infection and infectious sinusitis. The study was conducted to determine the seroprevalence of MS infection with associated risk factors and identification of MS organism in unvaccinated flocks of commercial breeder farms of the Chittagong district, Bangladesh.

Materials and Methods: A total of 365 serum samples were collected and tested for MS using serum plate agglutination (SPA) test for determination of MS seroprevalence. On the other hand, tracheal swabs were collected from each seropositive flocks for polymerase chain reaction (PCR) to determine the presence of MS organism.

Results: Among the farms, the highest prevalence was found to be 69% and the lowest prevalence was 28% with the average 60%. The seroprevalence of MS infection in breeder farms was highest 70% with the flock size >10,000 birds, whereas it was lowest 57% in the flocks ranging from 4000 to 7000. According to age group, the prevalence was found highest 70% in >60 weeks age group of birds and lowest 42% in 10-19 weeks group. The seroprevalence of MS in winter season was found as highest as 64%, whereas it was found lowest 60% in the summer season. There was a statistically significant difference (p<0.01) among the seroprevalence of MS in different breeder farms, flock size, and age groups, but there was no significant (p>0.05) difference in the winter, summer, and rainy season. To confirm the presence of MS in the samples, PCR test was applied using specific primers to amplify a 214 bp region of the 16S rRNA gene of the organism. In PCR, all seropositive flocks showed a positive result for MS.

Conclusion: As the plate agglutination test result showed 100% similar with PCR result, it can be suggested that agglutination test is better than molecular and culture techniques for MS detection and it is also cheaper and less time-consuming method.

Keywords: breeder farm, Mycoplasma synoviae, polymerase chain reaction, respiratory infection, risk factors, seroprevalence.

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