Veterinary World

     Open access and peer reviewed journal  

ISSN (Online): 2231-0916


Home l Editorial board l Instructions for authors l Reviewer guideline l Open access policy l Archives l FAQ

Open Access

Research (Published online: 14-10-2016)

6. Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes - D. J. Dutta, Himangshu Raj and Hiramoni Dev

Veterinary World, 9(10): 1070-1074



   doi: 10.14202/vetworld.2016.1070-1074



D. J. Dutta: Department of Veterinary Physiology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India;

Himangshu Raj: Department of Veterinary Physiology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India;

Hiramoni Dev: Department of Veterinary Physiology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India;


Received: 18-02-2016, Accepted: 28-08-2016, Published online: 14-10-2016


Corresponding author: D. J. Dutta, e-mail:

Citation: Dutta DJ, Raj H, Dev H (2016) Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes, Veterinary World, 9(10): 1070-1074.

Aim: The current study aims at investigating the polyadenylated (poly[A]) tail length of morphologically high and low competent oocytes at different developmental stages. Furthermore, effect of ultra-rapid vitrification on the poly(A) tail length was studied.

Materials and Methods: Fresh bovine cumulus oocyte complexes from abattoir originated ovaries were graded based on morphological characters and matured in vitro. Cryopreservation was done by ultra-rapid vitrification method. mRNA was isolated from different categories of oocyte and subjected to ligation-mediated poly(A) test followed by polymerase chain reaction for determining the poly(A) tail length of β actin, gap junction protein alpha 1 (GJA1), poly(A) polymerase alpha (PAPOLA), and heat shock 70 kDa protein (HSP70) transcripts.

Results: GJA1, PAPOLA, and HSP70 showed significantly higher poly(A) in immature oocytes of higher competence irrespective of vitrification effects as compared to mature oocytes of higher competence.

Conclusion: mRNA poly(A) tail size increases in developmentally high competent immature bovine oocytes. There was limited effect of ultra-rapid vitrification of bovine oocytes on poly(A).

Keywords: ligation-mediated poly(A) test, oocyte, poly-adenylated tail, vitrification.

1. Colgan, D.F. and Manley, J.L. (1997) Mechanism and regulation of mRNA polyadenylation. Genes Dev., 11(21): 2755-2766.
2. Reyes, J.M. and Ross, P.J. (2016) Cytoplasmic polyadenilation in mammalian oocyte maturation. Wiley Interdiscip. Rev. RNA., 7(1): 71-89.
3. Pawlicki, J.M. and Steitz, J.A. (2010) Nuclear networking fashions premessenger RNA and primary microRNA transcripts for function. Trends Cell Biol., 20: 52-61.
PMid:20004579 PMCid:PMC2821161
4. Groisman, I., Jung, M.Y., Sarkissian, M., Cao, Q. and Richter, J.D. (2002) Translational control of the embryonic cell cycle. Cell, 109: 473-483.
5. Novoa, I., Gallego, J., Ferreira, P.G. and Mendez, R. (2010) Mitotic cell cycle progression is regulated by CPEB1 and CPEB4-dependent translational control. Nat. Cell Biol., 12: 447-456.
6. Kojima, S., Sher-Chen, E.L. and Green, C.B. (2012) Circadian control of mRNA polyadenylation dynamics regulates rhythmic protein expression. Genes Dev., 26: 2724-2736.
PMid:23249735 PMCid:PMC3533077
7. Weill, L., Belloc, E., Bava, F.A. and Méndez, R. (2012) Translational control by changes in poly(A) tail length: Recycling mRNAs. Nat. Struct. Mol. Biol., 19: 577-585.
8. Janicke, A., Vancuylenberg, J., Boag, P.R., Traven, A. and Beilharz, T.H. (2012) ePAT: A simple method to tag adenylated RNA to measure poly(A)-tail length and other 3́ RACE applications. RNA., 18(6): 1289-1295.
PMid:22543866 PMCid:PMC3358650
9. Eckmann, C.R., Rammelt, C. and Wahle, E. (2011) Control of poly(A)tail length. Wiley Interdiscip. Rev. RNA., 2: 348-361.
10. Salles, F.J. and Strickland, S. (1995) Rapid and sensitive analysis of RNA polyadenylation states by PCR. PCR Methods Appl., 4: 317-321.
11. Beilharz, T.H. and Preiss, T. (2007) Widespread use of poly(A) tail length control to accentuate expression of the yeast transcriptome. RNA, 13: 982-997.
PMid:17586758 PMCid:PMC1894919
12. Dagley, M.J., Gentle, I.E., Beilharz, T.H., Pettolino, F.A., Djordjevic, J.T., Lo, T.L., Uwamahoro, N., Rupasinghe, T., Tull, D.L., McConville, M., Beaurepaire, C., Nantel, A., Lithgow, T., Mitchell, A.P. and Traven, A. (2011) Cell wall integrity is linked to mitochondria and phospholipid homeostasis in Candida albicans through the activity of the post-transcriptional regulator Ccr4-Pop2. Mol. Microbiol., 79: 968-989.
13. Bols, P.E.J., Jorsen, E.P.A., Goovaerts, I.G.F., Langbeen, A. and Leroy, J.L.M. (2012) High throughput non-invasive oocyte quality assessment: The search continues. Anim. Reprod., 9(3): 420-425.
14. Brevini, T.A., Lonergan, P., Cillo, F., Francisci, C., Favetta, L.A., Fair, T. and Gandolfi, F. (2002) Evolution of mRNA polyadenylation between oocyte maturation and first embryonic cleavage in cattle and its relation with developmental competence. Mol. Reprod. Dev., 63: 510-517.
15. Bilodeau-Goeseels, S. and Schultz, G.A. (1997) Changes in the relative abundance of various housekeeping gene transcripts in vitro produced early bovine embryos. Mol. Reprod. Dev., 47: 413-420.<413::AID-MRD7>3.0.CO;2-N
16. Brevini-Gandolfi, T.A.L., Favetta, L.A., Mauri, L., Luciano, A.M., Cillo, F. and Gandolfi, F. (1999) Changes in poly(A) tail length of maternal transcripts during in vitro maturation of bovine oocytes and their relation with developmental competence. Mol. Reprod. Dev., 52: 427-433.<427::AID-MRD12>3.0.CO;2-G
17. Lianoglou, S., Garg, V., Yang, J.L., Leslie, C.S. and Mayr, C. (2013) Ubiquitously transcribed genes use alternative polyadenylation to achieve tissue-specific expression. Genes Dev., 27: 2380-2396.
PMid:24145798 PMCid:PMC3828523
18. Wickens, M., Anderson, P. and Jackson, R.J. (1997) Life and death in the cytoplasm - Messages from the 30 end. Curr. Opin. Genet. Dev., 7: 220-232.
19. Renard, J.P., Baldacci, P., Richoux, D.V., Pournin, S. and Babinet, C. (1994) A maternal factor affecting mouse blastocyst formation. Development, 120: 797-802.
20. Succu, S., Bebbere, D., Bogliolo, L., Ariu, F., Fois, S., Giuseppeleoni, G., Berlinguer, F., Naitana, S. and Ledda, A. (2008) Vitrification of in vitro matured ovine oocytes affects in vitro pre-implantation development and mRNA abundance. Mol. Reprod. Dev., 75: 538-546.
21. Monzo, C., Haouzi, D., Roman, K., Assou, S., Dechaud, H. and Hamamah, S. (2012) Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes. Hum. Reprod., 27(7): 2160-2168.
22. Dessolle, L., Darai, E. and Cornet, D. (2009) Determinants of pregnancy rate in the donor oocyte model: A multivariate analysis of 450 frozen-thawed embryo transfers. Hum. Reprod., 24: 3082-3089.
23. Piccioni, F., Zappavigna, V. and Verrotti, A.C. (2005) Translational regulation during oogenesis and early development: The cap-poly(A) tail relationship. Crit. Rev. Biol., 328: 863-881.