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Research (Published online: 14-10-2016)

6. Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes - D. J. Dutta, Himangshu Raj and Hiramoni Dev

Veterinary World, 9(10): 1070-1074

 

 

   doi: 10.14202/vetworld.2016.1070-1074

 

 

D. J. Dutta: Department of Veterinary Physiology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India; duttadj@hotmail.com

Himangshu Raj: Department of Veterinary Physiology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India; xrajxyz@yahoo.co.in

Hiramoni Dev: Department of Veterinary Physiology, Faculty of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati - 781 022, Assam, India; drhsd@rediffmail.com

 

Received: 18-02-2016, Accepted: 28-08-2016, Published online: 14-10-2016

 

Corresponding author: D. J. Dutta, e-mail: duttadj@hotmail.com


Citation: Dutta DJ, Raj H, Dev H (2016) Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes, Veterinary World, 9(10): 1070-1074.



Aim: The current study aims at investigating the polyadenylated (poly[A]) tail length of morphologically high and low competent oocytes at different developmental stages. Furthermore, effect of ultra-rapid vitrification on the poly(A) tail length was studied.

Materials and Methods: Fresh bovine cumulus oocyte complexes from abattoir originated ovaries were graded based on morphological characters and matured in vitro. Cryopreservation was done by ultra-rapid vitrification method. mRNA was isolated from different categories of oocyte and subjected to ligation-mediated poly(A) test followed by polymerase chain reaction for determining the poly(A) tail length of β actin, gap junction protein alpha 1 (GJA1), poly(A) polymerase alpha (PAPOLA), and heat shock 70 kDa protein (HSP70) transcripts.

Results: GJA1, PAPOLA, and HSP70 showed significantly higher poly(A) in immature oocytes of higher competence irrespective of vitrification effects as compared to mature oocytes of higher competence.

Conclusion: mRNA poly(A) tail size increases in developmentally high competent immature bovine oocytes. There was limited effect of ultra-rapid vitrification of bovine oocytes on poly(A).

Keywords: ligation-mediated poly(A) test, oocyte, poly-adenylated tail, vitrification.



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