Open Access
Research
(Published
online: 18-10-2016)
9.
Morphological and molecular
identification of the brown dog tick Rhipicephalus sanguineus
and the camel tick Hyalomma dromedarii (Acari:
Ixodidae) vectors of Rickettsioses in Egypt -
Hend H. A. M. Abdullah, Amal El-Molla, Fayez A. Salib, Nesreen
A. T. Allam, Alaa A. Ghazy
and Sobhy Abdel-Shafy
Veterinary World, 9(10): 1087-1101
doi:
10.14202/vetworld.2016.1087-1101
Hend H. A. M.
Abdullah :
Department of
Parasitology and Animal Diseases, Division of Veterinary
Research, National Research Centre, Dokki, Giza, Egypt;
vet_nrc_2006@yahoo.com
Amal El-Molla :
Department of
Infectious Diseases, Faculty of Veterinary Medicine, Cairo
University, Giza, Egypt;
hatemibrahim15@gmail.com
Fayez A. Salib :
Department of
Infectious Diseases, Faculty of Veterinary Medicine, Cairo
University, Giza, Egypt;
fayez_vetmed@hotmail.com
Nesreen A. T.
Allam :
Department of
Parasitology and Animal Diseases, Division of Veterinary
Research, National Research Centre, Dokki, Giza, Egypt;
nesreenallam_nrc@yahoo.com
Alaa A. Ghazy :
Department of
Parasitology and Animal Diseases, Division of Veterinary
Research, National Research Centre, Dokki, Giza, Egypt;
aaghazy7@hotmail.com
Sobhy Abdel-Shafy :
Department of
Parasitology and Animal Diseases, Division of Veterinary
Research, National Research Centre, Dokki, Giza, Egypt; aasobhy@yahoo.com
Received: 10-06-2016, Accepted: 29-08-2016, Published online:
18-10-2016
Corresponding author:
Hend H.A.M.
Abdullah, e-mail: vet_nrc_2006@yahoo.com
Citation:
Abdullah HHAM, El-Molla A, Salib FA, Allam NAT, Ghazy AA,
Abdel-Shafy S (2016) Morphological and molecular identification
of the brown dog tick Rhipicephalus sanguineus and the
camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors
of Rickettsioses in Egypt, Veterinary World, 9(10):
1087-1101.
Abstract
Aim:
Rickettsioses have an epidemiological importance that includes
pathogens, vectors, and hosts. The dog tick Rhipicephalus
sanguineus and the camel tick Hyalomma dromedarii
play important roles as vectors and reservoirs of Rickettsiae.
The aim of this study was to determine the prevalence of
Rickettsiae in ixodid ticks species infesting dogs and camels in
Egypt, in addition to, the morphological and molecular
identification of R. sanguineus and H. dromedarii.
Materials and
Methods:
A total of 601
and 104 of ticks’ specimens were collected from dogs and camels,
respectively, in Cairo, Giza and Sinai provinces. Hemolymph
staining technique and OmpA and gltA genes
amplification were performed to estimate the prevalence rate of
Rickettsiae in ticks. For morphological identification of tick
species, light microscope (LM) and scanning electron microscope
(SEM) were used. In addition to the phylogenetic analyses of 18S
rDNA, Second internal transcript spacer, 12S rDNA, cytochrome c
oxidase subunit-1, and 16S rDNA were performed for molecular
identification of two tick species.
Results:
The
prevalence rate of Rickettsiae in ticks was 11.6% using
hemolymph staining technique and 6.17% by OmpA and
gltA genes amplification. Morphological identification
revealed that 100% of dogs were infested by R.
sanguineus while 91.9% of camels had been infested by H.
dromedarii. The phylogenetic analyses of five DNA markers
confirmed morphological identification by LM and SEM. The two
tick species sequences analyses proved 96-100% sequences
identities when compared with the reference data in Genbank
records.
Conclusion:
The
present studies confirm the suitability of mitochondrial DNA
markers for reliable identification of ticks at both intra- and
inter-species level over the nuclear ones. In addition to, the
detection of Rickettsiae in both ticks’ species and
establishment of the phylogenetic status of R. sanguineus
and H. dromedarii would be useful in understanding the
epidemiology of ticks and tick borne rickettsioses in Egypt.
Keywords:
hard ticks, light microscope, phylogenetic analysis, polymerase
chain reaction, Rickettsiae, scanning electron microscope.
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