Veterinary World

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Research (Published online: 23-09-2016)

14. The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration - Muslim Akmal, Tongku Nizwan Siregar, Sri Wahyuni, Hamny, Mustafa Kamal Nasution, Wiwik Indriati, Budianto Panjaitan and Dwinna Aliza

Veterinary World, 9(9): 1001-1005



   doi: 10.14202/vetworld.2016.1001-1005



Muslim Akmal: Laboratory of Histology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;

Tongku Nizwan Siregar: Laboratory of Reproduction, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;

Sri Wahyuni: Laboratory of Research, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia; Laboratory of Anatomy, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;

Hamny: Laboratory of Anatomy, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;

Mustafa Kamal Nasution: Department of PGMI , Faculty of Tarbiyah, STAIN Gajah Putih Takengon, Aceh Tengah, Aceh, Indonesia;

Wiwik Indriati: Student at Veterinary Public Health Graduate Program, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;

Budianto Panjaitan: Laboratory of Clinic, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;

Dwinna Aliza: Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia;


Received: 30-03-2016, Accepted: 10-08-2016, Published online: 23-09-2016


Corresponding author: Budianto Panjaitan, e-mail:

Citation: Akmal M, Siregar TN, Wahyuni S, Hamny, Nasution MK, Indriati W, Panjaitan B, Aliza D (2016) The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration, Veterinary World, 9(9): 1001-1005.

Aim: This study aims to determine the effect of seminal vesicle extract on cyclic adenosine monophosphate responsive element modulator (CREM) expression in rat Sertoli cells.

Materials and Methods: This study examined the expression of CREM on 20 male rats (Rattus norvegicus) at 4 months of age, weighing 250-300 g. The rats were divided into four groups: K0, KP1, KP2, and KP3. K0 group was injected with 0.2 ml normal saline; KP1 was injected with 25 mg cloprostenol (Prostavet C, Virbac S. A); KP2 and KP3 were injected with 0.2 and 0.4 ml seminal vesicle extract, respectively. The treatments were conducted 5 times within 12-day interval. At the end of the study, the rats were euthanized by cervical dislocation; then, the testicles were necropsied and processed for histology observation using immunohistochemistry staining.

Results: CREM expression in rat Sertoli cells was not altered by the administration of either 0.2 or 0.4 ml seminal vesicle extract.

Conclusion: The administration of seminal vesicle extract is unable to increase CREM expression in rat Sertoli cells.

Keywords: cyclic adenosine monophosphate responsive element modulator, seminal vesicle extract, Sertoli cells, spermatogenesis.

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