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Research (Published online: 29-09-2016)

20. Molecular characterization of Fasciola hepatica and phylogenetic analysis based on mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I and cytochrome oxidase subunit I) genes from the North-East of Iran - Saber Reaghi, Ali Haghighi, Majid Fasihi Harandi, Adel Spotin, Kourosh Arzamani and Soheila Rouhani

Veterinary World, 9(9): 1034-1038

 

 

   doi: 10.14202/vetworld.2016.1034-1038

 

 

Saber Reaghi: Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; saberraeghi@gmail.com

Ali Haghighi: Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; ahaghighi110@yahoo.com

Majid Fasihi Harandi: Research Center for Hydatid disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; majid.fasihi@gmail.com

Adel Spotin: Department of Parasitology, Medical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran; adelespotin@gmail.com

Kourosh Arzamani: Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran; arzamanik@gmail.com

Soheila Rouhani: Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; srouhani11@sbmu.ac.ir

 

Received: 03-06-2016, Accepted: 17-08-2016, Published online: 29-09-2016

 

Corresponding author: Soheila Rouhani, e-mail: srouhani11@sbmu.ac.ir


Citation: Reaghi S, Haghighi A, Harandi MF, Spotin A, Arzamani K, Rouhani S (2016) Molecular characterization of Fasciola hepatica and phylogenetic analysis based on mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I and cytochrome oxidase subunit I) genes from North-East of Iran, Veterinary World, 9(9): 1034-1038.



Aim: Fascioliasis is one of the most zoonotic diseases with global extension. As the epidemiological distribution of Fasciola may lead to various genetic patterns of the parasite, the aim of this study is to identify Fasciola hepatica based on spermatogenesis, and phylogenetic analysis using mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I [ND1] and cytochrome oxidase subunit I) gene marker.

Materials and Methods: In this study, 90 F. hepatica collected from 30 cattle at slaughterhouse located in three different geographical locations in the North-East of Iran were evaluated based on spermatogenetic ability and internal transcribed spacer 1 gene restriction fragment length polymorphism pattern. Genetic diversity and phylogenetic relationship using mtDNA gene marker for the isolates from the North-East of Iran, and other countries were then analyzed.

Results: Partial sequences of mtDNA showed eight haplotypes in both genes. The phylogenic analysis using neighbor joining as well as maximum likelihood methods showed similar topologies of trees. Pairwise fixation index between different F. hepatica populations calculated from the nucleotide data set of ND1 gene are statistically significant and show the genetic difference.

Conclusion: F. hepatica found in this region of Iran has different genetic structures through the other Fasciola populations in the world.

Keywords: Cytochrome oxidase subunit I, Fasciola hepatica, Iran, molecular characterization, nicotiamide adenine dinucleotide dehydrogenase subunit I, phylogenic.



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