Open Access
Research
(Published
online: 11-09-2016)
7.
Species determination using the red blood
cells morphometry in domestic animals -
Nezar Adili, Mohamed Melizi and Hadj Belabbas
Veterinary World, 9(9): 960-963
doi:
10.14202/vetworld.2016.960-963
Nezar Adili:
Department of Veterinary Medicine, Institute of Veterinary
Sciences and Agricultural Sciences, University of Batna 1, Batna
05000, Algeria; nezar.adili@yahoo.fr
Mohamed Melizi:
Department of Veterinary Medicine, Institute of Veterinary
Sciences and Agricultural Sciences, University of Batna 1, Batna
05000, Algeria; Mohamed.melizi@univ-batna.dz
Hadj Belabbas:
Department of Microbiology and Biochemistry, Faculty of
Sciences, University of Mohamed BOUDIAF, M’sila 28000, Algeria;
anavet.belabbas@yahoo.fr
Received: 22-12-2015, Accepted: 01-08-2016, Published online:
11-09-2016
Corresponding author:
Nezar Adili, e-mail: nezar.adili@yahoo.fr
Citation:
Adili N, Melizi M, Belabbas H (2016) Species determination using
the red blood cells morphometry in domestic animals,
Veterinary World, 9(9):
960-963.
Abstract
Aim:
This investigation is placed in the context of continuity of a
preliminary study already published; it was conducted in cattle,
sheep, goats, horses, and dogs; the main aim is to reveal and
develop criteria for the animal species determination based on
the morphometric parameters of red blood cells.
Materials and Methods:
Blood samples were taken from the jugular vein; and the smears
were confectioned on slides immediately after the blood
collection and stained according to the May-Gründwald Giemsa
method. For the morphometric study, three parameters were
considered which are: The diameter, the circumference, and the
surface of erythrocytes; and measurements were achieved using
the OPTIKATM Vision Pro software. Statistical analysis was
performed by both analysis of variances and Student’s t
analytical tests.
Results:
The recorded data showed that the three morphometric parameters
of red blood cells are higher in dogs followed, respectively, by
those of horses, cattle, and sheep, whereas, the goats have the
lowest ones. In addition, the obtained results allowed us to
propose new reference values for the circumference and the
surface of erythrocyte in considered species.
Conclusion:
This investigation permit concluding that from a drop of blood
it is possible to characterize the different animal species,
taking into account the diameter, the circumference, and the
surface of erythrocytes.
Keywords:
determination, morphometry, red blood cell, species.
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