Sources of contamination, prevalence, and antimicrobial resistance of thermophilic Campylobacter isolated from turkeys

Vet World Vol.11 August-2018 Article-9

Research Article

Veterinary World, 11(8): 1074-1081

https://doi.org/10.14202/vetworld.2018.1074-1081

Radia Bouhamed¹, Leila Bouayad¹, Sara Messad¹, Safia Zenia¹, Malek Naim², and Taha-Mossadak Hamdi¹

1. Laboratory of Food Hygiene and Quality Insurance System, High National Veterinary School, Rue Issad Abbes, 16111 El Alia, Oued Smar, Algiers, Algeria.
2. Department of Microbiology, Central Military Hospital, 16050 Kouba, Algiers, Algeria.

Background and Aim: Sources of contamination, prevalence, and antimicrobial susceptibility of thermophilic Campylobacter isolated from turkey samples were determined.

Materials and Methods: A total of 300 samples were collected from 3 farms (fecal droppings) and 4 poultry slaughterhouses (neck skins and ceca) located in the middle area of Algeria (Algiers, Boumerdes, and Bouira). After detection, an antibiogram was realized only for slaughterhouses samples.

Results: Samples from cecum (90.0%, 90/100; 95% confidence interval (CI)=84.1-95.9%), fecal dropping (68.0%, 68/100; 95% CI=58.9-77.1%), and neck skin (55.0%, 55/100; 95% CI=45.2-64.8%) were positive for thermophilic Campylobacter (p<0.05). Contamination rate of turkey carcasses was higher in modern slaughterhouse (96.7%) than in traditional slaughterhouses (37.1%) (p<0.05). Isolated strains were resistant to nalidixic acid (NA) (87.5%), tetracycline (TE) (81.3%), ciprofloxacin (CIP) (75.0%), ampicillin (AM) (65.6%), and erythromycin (25.0%) (p<0.05). 96.9% (124/128) of the isolates were multiresistant and 18 drug resistance patterns were registered. The predominant one (43.0%) was AM, NA, CIP, and TE. Conclusions: Potential sources of contamination of this fastidious bacterium were noticed in farms and slaughterhouses. Modern slaughterhouse allowed contamination of turkey carcasses more than a traditional slaughterhouse. However, the scalding step could not represent a source of contamination. The most tested strains exhibited resistance to erythromycin and/or CIP. It is worrisome because these molecules are considered as first-choice antibiotics for human campylobacteriosis. Keywords: antimicrobial resistance, farm, slaughterhouse, Thermophilic Campylobacter, turkey.

Keywords: antimicrobial resistance, farm, slaughterhouse, Thermophilic Campylobacter, turkey.

How to cite this article: Bouhamed R, Bouayad L, Messad S, Zenia S, Naim M, Hamdi TM (2018) Sources of contamination, prevalence, and antimicrobial resistance of thermophilic Campylobacter isolated from turkeys. Veterinary World, 11(8):1074-1081.

Received: 02-05-2018 Accepted: 05-07-2018 Published online: 07-08-2018

Corresponding author: Radia Bouhamed E-mail: bouhamed.r@gmail.com

DOI: 10.14202/vetworld.2018.1074-1081

Copyright: Bouhamed, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.