Vet World Vol.11 October-2018 Article-11
Research Article
Veterinary World, 11(10): 1428-1432
https://doi.org/10.14202/vetworld.2018.1428-1432
In vitro antibacterial effects of zinc oxide nanoparticles on multiple drug-resistant strains of Staphylococcus aureus and Escherichia coli: An alternative approach for antibacterial therapy of mastitis in sheep
2. Department of Applied Physical Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan.
Background and Aim: The aim of the study was to evaluate the antibacterial effects of zinc oxide nanoparticles (ZnO-NPs) and its possible alternative use for the treatment for mastitis in sheep and to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ZnO-NPs against multidrug-resistant Staphylococcus aureus and Escherichia coli strains isolated from subclinical mastitis cases in sheep.
Materials and Methods: A total of 50 pooled milk samples were collected from ewes with subclinical mastitis. Milk samples were cultured using standard laboratory techniques, and multidrug-resistant bacterial strains were determined using the Kirby-Bauer disk diffusion method. The MIC and MBC of ZnO-NPs were determined against isolated multidrug-resistant S. aureus and E. coli strains using microwell dilution method.
Results: A total of 43 different bacterial isolates were recovered from milk samples of ewes affected with subclinical mastitis. Isolated strains of S. aureus and E. coli were found resistant to three or more common antibacterial agents and were used to determine the MIC and MBC of ZnO-NPs. The MIC and MBC values of ZnO-NPs were significantly lower for S. aureus than that for E. coli. The MIC and MBC of ZnO-NPs against S. aureus were 3.9 μg/ml and 7.81 μg/ml, respectively, while for E. coli, the MIC and MBC of ZnO-NPs were 31.25 μg/ml and 62.5 μg/ml, respectively.
Conclusion: Results of this study indicate the potential antibacterial effects of ZnO-NPs against multidrug-resistant S. aureus and E. coli isolated from ovine subclinical mastitis at concentrations of 3.9 μg/ml and 31.25 μg/ml, respectively. Keywords: alternative therapy, antibiotics, mastitis, nanotechnology, sheep, zinc oxide.
Keywords: alternative therapy, antibiotics, mastitis, nanotechnology, sheep, zinc oxide.
How to cite this article: Alekish M, Ismail ZB, Albiss B, Nawasrah S (2018) In vitro antibacterial effects of zinc oxide nanoparticles on multiple drug-resistant strains of Staphylococcus aureus and Escherichia coli: An alternative approach for antibacterial therapy of mastitis in sheep, Veterinary World, 11(10): 1428-1432.
Received: 04-05-2018 Accepted: 12-09-2018 Published online: 16-10-2018
Corresponding author: Myassar Alekish E-mail: moalekish@just.edu.jo
DOI: 10.14202/vetworld.2018.1428-1432
Copyright: Alekish, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.