Histological classification and expression of markers of canine mast cell tumors

Vet World Vol.13 August-2020 Article-20

Research Article

Veterinary World, 13(8): 1627-1634

https://doi.org/10.14202/vetworld.2020.1627-1634

V. S. Cruz¹, J. C. A. Borges¹, L. L. Nepomuceno¹, P. A. M. Gonçalves¹, Y. C. L. Prado², C. Bianchi³, M. C. S. Fioravanti¹, and E. G. Araújo¹

1. Multi-User Laboratory for the Evaluation of Molecules Cells and Tissues, Veterinary and Zootechnical School of the Federal University of Goiás, Campus Samambaia, Avenida Esperança, Goiânia, GO 74690-900, Brazil.
2. Department of Veterinary Medicine of the University Center Nossa Senhora do Patrocínio, Pc Antônio Vieira Tavares, 73, Salto - SP, 13320-219, Brazil.
3. Department of Experimental Medicine of the University of Mogi das Cruzes, Av. Dr. Cândido X. de Almeida e Souza, 200 - Centro Cívico, Mogi das Cruzes - SP, 08780-911, Brazil.

Background and Aim: Mast cell tumors (MCTs) are malignant neoplasms that are common in dogs. Their biological behavior is variable and unpredictable. The aim of the present study was to analyze the histological classification and expression of markers of canine MCTs.

Materials and Methods: Thirty samples of canine MCTs were graded according to the histological classification methods of Patnaik and those of Kiupel. The expression of phosphoprotein 53 (p53) and c-kit proteins was quantified by immunohistochemistry using image processing software, ImageJ - a public domain computer program, developed at the National Institutes of Health.

Results: It was possible to determine the grade of 100% of the samples. According to Patnaik's classification, 20.00% of the samples were Grade 1, 43.30% were Grade 2, and 36.70% were Grade 3. According to Kiupel's classification, 56.67% of the samples were of high intensity and 43.33% were of low intensity. Grade 1 tumors had the highest expression of p53 and c-kit, and Grade 2 had the lowest expression. The results showed that it is necessary to perform both histological grading methods. The classification into high and low intensity may provide more consistent results than the three-level grading system. However, a smaller number of categories, although it facilitates the classification, may not be sufficient for the prognosis.

Conclusion: Quantitative evaluation of p-53 and c-kit expression is a useful tool to increase the accuracy of the analysis and to aid in choosing the treatment method for canine MCTs. Histological grading should be combined with other diagnostic methods. Keywords: Bismarck brown, hematoxylin and eosin, ImageJ, round cell tumor, toluidine blue.

Keywords: Bismarck brown, hematoxylin and eosin, ImageJ, round cell tumor, toluidine blue.

How to cite this article: Cruz VS, Borges JCA, Nepomuceno LL, Gonçalves PAM, Prado YCL, Bianchi C, Fioravanti MCS, Araújo EG (2020) Histological classification and expression of markers of canine mast cell tumors, Veterinary World, 13(8): 1627-1634.

Received: 11-04-2020 Accepted: 25-06-2020 Published online: 18-08-2020

Corresponding author: V. S. Cruz E-mail: vanessascruz@ufg.br

DOI: 10.14202/vetworld.2020.1627-1634

Copyright: Cruz, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.