A novel ex vivo porcine model of acid-induced esophageal damage for preliminary functional evaluations of anti-gastroesophageal reflux disease medical devices

Vet World Vol.13 December-2020 Article-18

Research Article

Veterinary World, 13(12): 2728-2735

https://doi.org/10.14202/vetworld.2020.2728-2735

Domenico Ventrella¹, Roberta Salaroli¹, Alberto Elmi¹, Giacomo Carnevali¹, Monica Forni¹, Fabio Baldi^2,3, and Maria Laura Bacci¹

1. Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia (BO), Italy.
2. Center for the Study of Diseases of the Esophagus, University of Bologna, Bologna, Italy.
3. GVM Care and Research, Cotignola (RA), Italy.

Background and Aim: The aim of the study was to set up a porcine ex vivo model of acid-induced damage and to evaluate its performance by means of multichannel intraluminal impedance and pH (MII-pH) live recording, histology, and Evans blue (EB) permeability assay.

Materials and Methods: Thirteen esophagi, collected at a slaughterhouse, were ablated of their sphincters, pinned upright on a support, and placed in a thermostatic hood at 37°C with two infusion tubes and an MII-pH probe inserted in the top end. Three esophagi (histology controls) were only left in the hood for 3.5 h before sampling, while the remaining organs underwent the experimental protocol including saline infusion and recovery recording, and acid solution infusion and recovery recording.

Results: MII-pH analysis highlighted a significantly stronger decrease during acid infusion when compared to saline, but a better post-infusion recovery for saline solution. At the end of the protocol, MII was still statistically lower than baseline. The acid-damaged esophagi significantly absorbed more EB dye, and histology revealed strong mucosal exfoliation.

Conclusion: The proposed model of esophageal acid damage seems to be repeatable, reliable, and achievable using organs collected at the slaughterhouse. MII recording proved to have good sensitivity in detecting mucosal alterations also in ex vivo trials. Keywords: esophagus ex vivo model, Evans blue permeability assay, gastroesophageal reflux disease, multichannel intraluminal impedance, pig.

Keywords: esophagus ex vivo model, Evans blue permeability assay, gastroesophageal reflux disease, multichannel intraluminal impedance, pig.

How to cite this article: Ventrella D, Salaroli R, Elmi A, Carnevali G, Forni M, Baldi F, Bacci ML (2020) A novel ex vivo porcine model of acid-induced esophageal damage for preliminary functional evaluations of anti-gastroesophageal reflux disease medical devices, Veterinary World, 13(12): 2728-2735.

Received: 20-07-2020 Accepted: 04-11-2020 Published online: 21-12-2020

Corresponding author: Alberto Elmi E-mail: alberto.elmi2@unibo.it

DOI: 10.14202/vetworld.2020.2728-2735

Copyright: Ventrella, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.