Vet World Vol.13 November-2020 Article-16
Research Article
Veterinary World, 13(11): 2411-2415
https://doi.org/10.14202/vetworld.2020.2411-2415
Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India
2. Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
Background and Aim: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. The organism can spread through the urine of infected animals, which can get into water or soil and can survive there for weeks to months. The study was undertaken to detect the pathogenic Leptospira in healthy dogs' urinary shedding by real-time polymerase chain reaction (qPCR).
Materials and Methods: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. To detect the pathogenic Leptospira organisms in dogs' urinary shedding, 239 urine samples were collected from healthy dogs from April 2018 to March 2019 from different areas of Haryana. All the urine samples were processed for DNA extraction and qPCR technique was used to detect the presence of Leptospira.
Results: Out of 239 urine samples of dogs, none of the samples resulted in the detection of DNA of pathogenic Leptospira organisms.
Conclusion: The present study indicated low risk of transmission of Leptospira organisms from dogs' urine to human beings in Haryana. Keywords: Leptospira, leptospirosis, qPCR, lipL32, Haryana.
Keywords: Leptospira, leptospirosis, qPCR, lipL32, Haryana.
How to cite this article: Rohilla P, Khurana R, Kumar A, Batra K, Gupta R (2020) Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India, Veterinary World, 13(11): 2411-2415.
Received: 23-07-2020 Accepted: 12-10-2020 Published online: 12-11-2020
Corresponding author: Preeti Rohilla E-mail: rohillapreeti807@gmail.com
DOI: 10.14202/vetworld.2020.2411-2415
Copyright: Rohilla, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.