The combined effect of magnetic and electric fields using on/off infrared light on the blood sugar level and the diameter of Langerhans islets of diabetic mice

Vet World Vol.13 October-2020 Article-34

Research Article

Veterinary World, 13(10): 2286-2293

https://doi.org/10.14202/vetworld.2020.2286-2293

S. Suhariningsih^1,2, Suryani Dyah Astuti^1,2, Saikhu Akhmad Husen³, Dwi Winarni³, Dian Astri Rahmawati³, Akhmad Taufiq Mukti⁴, Alfian Pramudita Putra^5,6, and Muhammad Miftahussurur⁷

1. Department of Physics, Faculty of Sciences and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
2. Biophysics and Medical Physics Research Group, Faculty of Sciences and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
3. Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia.
4. Department of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya 60115, Indonesia.
5. Biomedical Engineering Study Program, Department of Physics, Faculty of Sciences and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
6. Biomedical Signals and Systems Research Group, Faculty of Sciences and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
7. Department of Internal Medicine, Division of Gastroentero-Hepatology, Faculty of Medicine, Institute of Tropical Diseases, Universitas Airlangga, Surabaya, Indonesia.

Background and Aim: At present, diabetes is treated with oral antidiabetic medicines, such as sulfonylureas and thiazolidine, as well as insulin injection. However, these methods have several shortcomings. Therefore, alternatives for treating diabetes mellitus (DM) are needed. This study aims to determine the combined effect of magnetic and electric fields on blood sugar levels and the diameter of Langerhans islets of diabetic mice.

Materials and Methods: Induction of DM in mice was carried out by administering lard for 2 weeks and continued with an intraperitoneal injection of streptozotocin, dissolved in a 4.5 pH citrate buffer, and administered in a dose of 30 mg/kg bodyweight for 5 days. Treatments were used in combination with magnetic and electric fields using on/off infrared light. Blood samples were pipetted through the tip of mice's tails to establish the blood sugar level for each individual mouse. Histology preparation of the pancreas organ was affected using the histology standard as well as hematoxylin and eosin staining methods. Langerhans islet diameter data were analyzed using analysis of variance followed by Duncan's multiple range test. Data analysis was performed at α=0.05.

Results: The results showed that the combined treatment of permanent magnetic and unidirectional electric fields (PS) caused changes in blood sugar levels that were not significantly different from the normal control group. The PS treatment improved the diameter of the Langerhans islets but not to a significant degree compared to other treatments.

Conclusion: The use of PS treatment is effective for reducing the blood sugar levels of diabetic mice and improving the diameter of their Langerhans islets. Keywords: blood sugar level, diabetic mice, electric field, infrared light, Langerhans islets diameter, magnetic field.

Keywords: blood sugar level, diabetic mice, electric field, infrared light, Langerhans islets diameter, magnetic field.

How to cite this article: Suhariningsih S, Astuti SD, Husen SA, Winarni D, Rahmawati DA, Mukti AT, Putra AP, Miftahussurur M (2020) The combined effect of magnetic and electric fields using on/off infrared light on the blood sugar level and the diameter of Langerhans islets of diabetic mice, Veterinary World, 13(10): 2286-2293.

Received: 31-05-2020 Accepted: 16-09-2020 Published online: 31-10-2020

Corresponding author: Suryani Dyah Astuti E-mail: suryanidyah@fst.unair.ac.id

DOI: 10.14202/vetworld.2020.2286-2293

Copyright: Suhariningsih, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.