An immunohistochemical study of endocrine cells in the digestive tract of Varanus salvator (Reptile: Varanidae)

Vet World Vol.13 September-2020 Article-1

Research Article

Veterinary World, 13(9): 1737-1742

https://doi.org/10.14202/vetworld.2020.1737-1742

Mahfud Mahfud¹, Ernawati Ernawati¹, Nur R. Adawiyah Mahmud¹, Teguh Budipitojo², and Hery Wijayanto²

1. Department of Biology Education, University of Muhammadiyah Kupang, East of Nusa Tenggara Province, Indonesia.
2. Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Gajah Mada University, Yogyakarta, Indonesia.

Background and Aim: The aim of the study was to identify the distribution pattern and frequency of endocrine cell types in the digestive tract of Varanus salvator.

Materials and Methods: The presence of endocrine cells (glucagon, somatostatin, and serotonin) in the digestive tract (esophagus, stomach, and intestine) was detected using the avidin-biotin complex (ABC) method.

Results: Three types of endocrine cells immunoreactive to antisera glucagon, serotonin, and somatostatin were found in the caudal portion of the small and large intestines but were not observed in the esophagus, stomach, and caput and medial sections of the small intestine. Endocrine cells distributed in the digestive tract of V. salvator vary in color intensity, from weak to sharp, in response to the primer antibody.

Conclusion: Endocrine cells in the digestive tract that is immunoreactive to glucagon, somatostatin, and serotonin are those found in the caudal portion of the small and large intestines. They are varied in distribution pattern, frequency, and color intensity. Keywords: digestive tract, endocrine cell, immunohistochemistry, Varanus salvator.

Keywords: digestive tract, endocrine cell, immunohistochemistry, Varanus salvator.

How to cite this article: Mahfud M, Ernawati E, Mahmud NRA, Budipitojo T, Wijayanto H (2020) An immunohistochemical study of endocrine cells in the digestive tract of Varanus salvator (Reptile: Varanidae), Veterinary World, 13(9): 1737-1742.

Received: 17-02-2020 Accepted: 10-07-2020 Published online: 01-09-2020

Corresponding author: Teguh Budipitojo E-mail: budipitojo@ugm.ac.id

DOI: 10.14202/vetworld.2020.1737-1742

Copyright: Mahfud, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.