Vet World Vol.14 May-2021 Article-20
Research Article
Veterinary World, 14(5): 1210-1219
https://doi.org/10.14202/vetworld.2021.1210-1219
Prebiotics mannan-oligosaccharides accelerate sexual maturity in rats: A randomized preclinical study
2. Department of Functional Sciences, Laboratory of Microbiology, Faculty of Biological Sciences, Universidade do Oeste Paulista, Presidente Prudente, São Paulo, Brazil.
3. Department of Functional Sciences, Laboratory of Physiology, Faculty of Physiotherapy, Universidade do Oeste Paulista, Presidente Prudente, São Paulo, Brazil.
4. Department of Functional Sciences, Laboratory of Physiology, Faculty of Medicine, Universidade do Oeste Paulista, Presidente Prudente, São Paulo, Brazil.
5. Department of Pathology, Faculty of Veterinary Medicine, Universidade de Santo Amaro, São Paulo, Brazil.
6. Department of Reproduction, Faculty of Veterinary Medicine, Universidade do Oeste Paulista, Presidente Prudente, São Paulo, Brazil.
7. Department of Statistics, Faculty of Veterinary Medicine, Universidade do Oeste Paulista, São Paulo, Brazil.
Background and Aim: The prebiotics, mannan-oligosaccharides (MOS), demonstrate the ability to increase probiotic microorganisms and fixation and removal of pathogens associated with chronic systemic inflammation in the digestive system. Inflammatory processes play an important role in modulating the brain-intestinal axis, including maintaining male reproductive function and spermatogenesis and regulating stress. The aim of the present study was to evaluate the action of MOS on testosterone and corticosterone concentrations and the reproductive system development of rats in the growth phase as an animal model.
Materials and Methods: In total, 128 male rats were used, randomly divided into four experimental groups (n=32): Control; MOS 1; MOS 2; and MOS 3. From each group, eight animals were sacrificed in four experimental moments (14, 28, 42, and 56 days, respectively, moments 1, 2, 3, and 4) and hormonal measurements and histological evaluations were performed.
Results: The results revealed the effect of diet, MOS, and timing on testicle weight (p<0.05). At moments 3 and 4, the groups supplemented with MOS showed higher concentrations of testosterone and decreased corticosterone levels throughout the experimental period. Groups supplemented with MOS showed an increase in the frequency of relative sperm and sperm scores. The radii of the seminiferous tubules presented a significant statistical effect of the diet, moments, and diet + moment interaction.
Conclusion: It was concluded that the three different MOS prebiotics brought forward sexual maturity. Keywords: corticosterone, murine, prebiotic, reproductive system, testosterone, yeast.
Keywords: corticosterone, murine, prebiotic, reproductive system, testosterone, yeast.
How to cite this article: Rodrigues LE, Kishibe MM, Keller R, dos Santos Caetano HR, Rufino MN, de Carvalho Sanches O, Giometti IC, Giuffrida R, Bremer-Neto H (2021) Prebiotics mannan-oligosaccharides accelerate sexual maturity in rats: A randomized preclinical study, Veterinary World, 14(5): 1210-1219.
Received: 18-12-2020 Accepted: 24-03-2021 Published online: 20-05-2021
Corresponding author: Hermann Bremer-Neto E-mail: hermann@unoeste.br
DOI: 10.14202/vetworld.2021.1210-1219
Copyright: Rodrigues, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.