Open Access
Research (Published online: 18-04-2023)
16. The first study on antimicrobial resistance of Staphylococcus aureus isolated from raw goat milk associated with subclinical mastitis in Siliragung Subdistrict, East Java, Indonesia
Ratih Novita Praja, Aditya Yudhana, Amung Logam Saputro, and Jonathan Mark Hamonangan
Veterinary World, 16(4): 786-791

Ratih Novita Praja: Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia; Department of Veterinary Science, Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo Street, Surabaya, East Java, Indonesia.
Aditya Yudhana: Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia; Department of Veterinary Science, Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo Street, Surabaya, East Java, Indonesia.
Amung Logam Saputro: Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia; Department of Veterinary Science, Division of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo Street, Surabaya, East Java, Indonesia.
Jonathan Mark Hamonangan: Veterinary Medicine Study Program, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia.

doi: 10.14202/vetworld.2023.786-791

Article history: Received: 21-12-2022, Accepted: 08-03-2023, Published online: 18-04-2023

Corresponding authors: Ratih Novita Praja

E-mail: ratihnovitapraja@fkh.unair.ac.id

Citation: Praja RN, Yudhana A, Saputro AL, and Hamonangan JM (2023) The first study on antimicrobial resistance of Staphylococcus aureus isolated from raw goat milk associated with subclinical mastitis in Siliragung Subdistrict, East Java, Indonesia. Veterinary World, 16(4): 786-791.
Abstract

Background and Aim: Raw goat milk is a highly nutritious dairy product and a suitable medium for the growth of antimicrobial-resistant Staphylococcus aureus, the leading cause of subclinical mastitis. This study aimed to investigate the resistance status of S. aureus which isolated from goat milk associated with subclinical mastitis cases in Siliragung Subdistrict, Banyuwangi District, East Java, Indonesia.

Materials and Methods: The S. aureus isolates were recovered from 258 raw goat milk samples from seven different dairy goat farms. Preliminary screening of subclinical mastitis using the California mastitis test, then samples with score +3 and +4 were taken for further isolation and identification, followed by a biochemical test to determine the S. aureus. Moreover, the bacteria susceptibility test against several antimicrobials was done using the disk diffusion method.

Results: Based on our findings, a total of 66 (25.58%) raw goat milk samples were tested positive for S. aureus, of which 36.36% were identified as multidrug-resistant. Moreover, S. aureus were also identified as resistant to penicillin (81.82%), ampicillin (65.15%), erythromycin (50.52%), and gentamicin (36.09%).

Conclusion: The prevalence of S. aureus isolated from raw goat milk associated with subclinical mastitis in Siliragung Subdistrict, Banyuwangi District, Indonesia, was recorded at 25.58%. Moreover, 36.36% of S. aureus isolates were categorized as resistant to three or more classes of antibiotics. The biosafety and biosecurity procedures during the milking process should be strengthened in dairy goat farms to prevent the transmission of antimicrobial resistance among animals, humans, and environments.

Keywords: antimicrobial resistance, food security, infectious disease, public health, tropical disease.