Molecular prevalence of Anaplasma spp. in cattle and assessment of associated risk factors in Northeast Thailand

Vet World Vol.16 August-2023 Article-17

Research Article

Veterinary World, 16(8): 1702-1707

https://doi.org/10.14202/vetworld.2023.1702-1707

Tossapol Seerintra¹, Bhuripit Saraphol¹, Tongjit Thanchomnang², and Supawadee Piratae^3,4

1. Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, 44000, Thailand.
2. Faculty of Medicine, Mahasarakham University, Maha Sarakham, 44000, Thailand.
3. One Health Research Unit, Mahasarakham University, Maha Sarakham, 44000, Thailand.
4. Veterinary Infectious Disease Research Unit, Mahasarakham University, Maha Sarakham, 44000, Thailand.

Background and Aim: Anaplasma spp. are common rickettsia species described in ruminant hosts, including cattle. The clinical signs of anaplasmosis range from asymptomatic to mortality. However, there are insufficient studies on epidemiology surveys of this blood pathogen. This study aimed to estimate the prevalence and risk factors of anaplasmosis in beef and dairy cattle in Northeast, Thailand.

Materials and Methods: A total of 187 blood samples of beef and dairy cattle were collected from five provinces in Northeast Thailand. Anaplasma spp. infections were screened by microscopic examination and polymerase chain reaction targeting specific genes (msp4 gene for Anaplasma marginale and 16S rRNA gene for Anaplasma platys and Anaplasma bovis). Moreover, the associated risk factors for the infections were evaluated.

Results: Overall, blood samples from cattle revealed that 17.6% (33/187) were positive for Anaplasma spp. by microscopic examination and 20.8% (39/187) were positive by DNA amplification. Of these 20.8%, 17.6% were A. marginale and 3.2% were A. platys. However, A. bovis infection was not detected. Infection with Anaplasma spp. and A. marginale showed a significant association with breed and gender (p < 0.05) while age and packed cell volume levels showed no significant statistical relationship between Anaplasma spp. infected and uninfected animals.

Conclusion: This study indicated that anaplasmosis is distributed in beef and dairy cattle in Thailand; therefore, prevention and control strategies for these pathogens should be improved. This information will benefit veterinarians and cowherds by avoiding vector exposure and eliminating tick breeding sites. Keywords: anaplasmosis, beef cattle, blood parasite, dairy cattle, molecular detection.

Keywords: anaplasmosis, beef cattle, blood parasite, dairy cattle, molecular detection.

How to cite this article: Seerintra T, Saraphol B, Thanchomnang T, and Piratae S (2023) Molecular prevalence of Anaplasma spp. in cattle and assessment of associated risk factors in Northeast Thailand, Veterinary World, 16(8): 1702-1707.

Received: 03-05-2023 Accepted: 24-07-2023 Published online: 24-08-2023

Corresponding author: Supawadee Piratae E-mail: supawadee.p@msu.ac.th

DOI: 10.14202/vetworld.2023.1702-1707

Copyright: Seerintra, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.