Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand

Vet World Vol.16 February-2023 Article-7

Research Article

Veterinary World, 16(2): 291-295

https://doi.org/10.14202/vetworld.2023.291-295

Kanpapat Boonchuay^1,2, Narin Sontigun^1,2,3, Tuempong Wongtawan^1,2,3, and Punpichaya Fungwithaya^1,2,3

1. Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand.
2. One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand.
3. Centre of Excellence Research for Melioidosis and Other Microorganisms, Walailak University, Nakhon Si Thammarat 80160, Thailand.

Background and Aim: Mammaliicoccus sciuri, formerly known as Staphylococcus sciuri, is an opportunistic pathogen in the environment, human and animal mucosa, and skin. Although this pathogen is becoming more resistant to drugs and harmful to animals and humans, basic knowledge of this pathogen remains limited. This study aimed to investigate a new multilocus sequencing type (MLST) related to the antibiotic resistance pattern of M. sciuri from animals in southern Thailand.

Materials and Methods: We used 11 methicillin-resistant M. sciuri (MRMS) isolates in this study which were obtained from six horses, four cows, and one chicken of the previous study. Antimicrobial resistance (AMR) was re-evaluated based on the minimum inhibitory concentration using the VITEK® 2 automated system. Three AMR genes were examined, namely mecA, mecC, and blaZ. Staphylococcal chromosomal cassette mec (SCCmec) gene detection was performed through the multiplex polymerase chain reaction (PCR). Internal segments of the seven housekeeping genes, ack, aroE, ftsZ, glpK, gmk, pta1, and tpiA, were used for multilocus sequence typing. The population of resistant bacteria and the types of multidrug-resistant, extensively drug-resistant, and pandemic drug-resistant bacteria were classified through descriptive analysis.

Results: mecA and blaZ genes were detected in all isolates; however, the mecC gene was not observed in any isolate based on the PCR results. All MRMS isolates revealed a non-typable SCCmec. Seven MLSTs (71, 81, 120, 121, 122, 199, and 200) were identified in this study.

Conclusion: The characteristics of MRMS in Southern Thailand were variable, particularly in cattle and horses. The antibiogram and SCCmec types of this pathogen remain concerns with regard to antibiotic-resistant gene transmission among Staphylococcus and Mammaliicoccus species. All MLSTs in Thailand revealed the distribution among clones in Asia, including the virulence of a zoonotic clone in Southern Thailand. Keywords: antibiogram, Mammaliicoccus sciuri, methicillin-resistant bacteria, multilocus sequencing types, resistant gene.

Keywords: antibiogram, Mammaliicoccus sciuri, methicillin-resistant bacteria, multilocus sequencing types, resistant gene.

How to cite this article: Boonchuay K, Sontigun N, Wongtawan T, and Fungwithaya P (2023) Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand, Veterinary World, 16(2): 291–295.

Received: 11-10-2022 Accepted: 13-01-2023 Published online: 15-02-2023

Corresponding author: Punpichaya Fungwithaya E-mail: punpichaya.fu@mail.wu.ac.th

DOI: 10.14202/vetworld.2023.291-295

Copyright: Boonchuay, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.