Antifungal activity of silver nanoparticles prepared using Aloe vera extract against Candida albicans

Vet World Vol.16 January-2023 Article-3

Research Article

Veterinary World, 16(1): 18-26

https://doi.org/10.14202/vetworld.2023.18-26

Mbarga Manga Joseph Arsène^1,2, Podoprigora Irina Viktorovna^1,2, Marukhlenko Alla³, Morozova Mariya³, Senyagin Alexander Nikolaevitch^1,2, Anyutoulou Kitio Linda Davares¹, Mumrova Evgenia Yurievna², Manar Rehailia⁴, Ada Arsene Gabin⁵, Kulikova A. Alekseevna⁶, Yashina Natalia Vyacheslavovna¹, Zhigunova Anna Vladimirovna¹, Orlova Svetlana⁷, and Das Milana¹

1. Department of Microbiology V.S. Kiktenko, Medical Institute, Peoples Friendship University of Russia (RUDN University), Moscow, Russia.
2. Research Institute of Molecular and Cellular Medicine, Peoples Friendship University of Russia (RUDN University), Moscow, Russia.
3. Department of Pharmaceutical and Toxicological Chemistry, Medical Institute, Peoples Friendship University of Russia (RUDN University), Moscow, Russia.
4. Department of Agrobiotechnology, Agrarian Institute, Peoples Friendship University of Russia (RUDN University), Moscow, Russia.
5. Department of Traumatology and Orthopedics, Medical Institute, Peoples Friendship University of Russia (RUDN University), Moscow, Russia.
6. Department of Oral and Maxillofacial Surgery, Medical Institute, Peoples Friendship University of Russia (RUDN University), Moscow, Russia.
7. Department of Dietetics and Clinical Nutritiology, Medical Institute, Peoples Friendship University of Russia (RUDN University), Moscow, Russia.

Background and Aim: Resistance to antifungal agents is a serious public health concern that has not been investigated enough because most studies on antimicrobials are dedicated to antibacterial resistance. This study aimed to synthesize silver nanoparticles (AgNPs) using Aloe vera extract, and to assess its antifungal activity against Candida albicans.

Materials and Methods: Silver nanoparticles were synthesized by reducing Ag nitrate with aqueous A. vera extracts. Physicochemical properties of synthesized AgNPs were determined by ultraviolet–visible spectrophotometry, photon cross-correlation spectroscopy, energy-dispersive X-ray fluorescence spectrometry, X-ray diffraction analysis, and Fourier-transform infrared spectroscopy. An antifungal investigation was performed against four clinical C. albicans (C1, C2, C3, and C4) and a reference strain, C. albicans ATCC 10321.

Results: Cubic AgNPs with a mean X50 hydrodynamic diameter of 80.31 ± 10.03 nm were successfully synthesized. These AgNPs exhibited maximum absorbance at 429.83 nm, and X-ray fluorescence (XRF) confirmed the presence of Ag in AgNPs solution by a characteristic peak in the spectrum at the Ag Kα line of 22.105 keV. Infrared spectra for AgNPs and A. vera extract indicated that the compounds present in the extract play an essential role in the coating/capping of synthesized AgNPs. Different concentrations (200, 100, 50, 25, 10, and 5 μg/mL) of AgNPs were tested. The antifungal activity was shown to be dose-dependent with inhibition zones ranging from 10 mm to 22 mm against C. albicans ATCC 10231, 0 mm to 15 mm against C1, 0 mm to 16 mm against C2 and C3, and 0 mm to 14 mm for C4. Minimum inhibitory concentration ranged from 16 μg/mL to 32 μg/mL against clinical C. albicans (C1, C2, C3, and C4) and was 4 μg/mL against C. albicans ATCC 10231.

Conclusion: This study showed the ability of A. vera to serve as an efficient reducing agent for the biogenic synthesis of AgNPs with excellent antifungal activity. Keywords: Aloe vera, antifungal, antimicrobials, green synthesis, silver nanoparticles.

Keywords: Aloe vera, antifungal, antimicrobials, green synthesis, silver nanoparticles.

How to cite this article: Arsène MMJ, Viktorovna PI, Alla M, Mariya M, Nikolaevitch SA, Davares AKL, Yurievna ME, Rehailia M, Gabin AA, Alekseevna KA, Vyacheslavovna YN, Vladimirovna ZA, Svetlana O, and Milana D (2023) Antifungal activity of silver nanoparticles prepared using Aloe vera extract against Candida albicans, Veterinary World, 16(1): 18–26.

Received: 23-08-2022 Accepted: 25-11-2022 Published online: 06-01-2023

Corresponding author: Mbarga Manga Joseph Arsène E-mail: josepharsenembarga@yahoo.fr

DOI: 10.14202/vetworld.2023.18-26

Copyright: Arsène MMJ, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.