The potential of lactic acid bacteria isolated from ikan budu (fermented fish) to inhibit the growth of pathogenic fungi and detoxify aflatoxin B1

Vet World Vol.16 July-2023 Article-1

Research Article

Veterinary World, 16(7): 1373-1379

https://doi.org/10.14202/vetworld.2023.1373-1379

Yetti Marlida¹, Nurmiati Nurmiati², Husmaini Husmaini³, Nurul Huda⁴, Lili Anggraini¹, and Laily Rinda Ardani⁵

1. Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Limau Manis Campus, Padang 25163, West Sumatra, Indonesia.
2. Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Padang City, West Sumatra 25175, Indonesia.
3. Department of Animal Production, Faculty of Animal Science, Andalas University, Limau Manis Campus, Padang 25163, West Sumatra, Indonesia.
4. Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, 90509, Sandakan, Sabah, Malaysia.
5. Graduate Program of Animal Science, Andalas University, Limau Manis Campus, Padang 25163, West Sumatra, Indonesia.

Background and Aim: Market demand for safe feed and food supply and consumer preferences for safe and healthy products are increasing. Control measures to counter threats to the feed supply need to be implemented as early as possible to prevent economic losses. Mycotoxins produced by certain groups of fungi are a problem that can disrupt the feed supply or pose a threat to the health of animals and humans. Biological control to detoxify contaminated feed ingredients can be carried out on a large scale economically. For example, lactic acid bacteria (LAB) can act as biological agents for eliminating mycotoxins. This study aimed to clarify the value of screening LAB to inhibit Aspergillus flavus> growth and detoxify aflatoxin B1 (AFB1).

Materials and Methods: In this study, using a completely randomized design with three replications, five isolates of LAB (LA.1, LA.6, LA.8, LA.12, and LA.22) along with their supernatants were tested qualitatively and quantitatively for their ability to counter mycotoxins using A. flavus and corn kernels. The isolates with the best activity were identified by sequencing 16S rDNA.

Results: The results showed that the five LAB isolates can inhibit the growth of A. flavus and detoxify AFB1. Among these isolates, LA.12 showed the best performance, followed by LA.22, LA.8, LA.6, and then LA.1. The sequencing results confirmed that LA.12 was Lactobacillus harbinensis strain 487.

Conclusion: All of the isolates in this study have the potential as biological agents for detoxifying AFB1, with isolate LA.12 appearing to be the most promising biodetoxification agent for feed (AFB1 in corn) based on its ability to inhibit pathogenic fungi. Keywords: biodetoxification, feedstuffs, lactic acid bacteria, pathogenic fungi.

Keywords: biodetoxification, feedstuffs, lactic acid bacteria, pathogenic fungi.

How to cite this article: Marlida Y, Nurmiati N, Husmaini H, Huda N, Anggraini L, and Ardani LR (2023) The potential of lactic acid bacteria isolated from ikan budu (fermented fish) to inhibit the growth of pathogenic fungi and detoxify aflatoxin B1, Veterinary World, 16(7): 1373-1379.

Received: 04-03-2023 Accepted: 23-05-2023 Published online: 04-07-2023

Corresponding author: Yetti Marlida E-mail: yettimarlida@ansci.unand.ac.id

DOI: 10.14202/vetworld.2023.1373-1379

Copyright: Marlida, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.