Open Access
Research (Published online: 19-07-2023)
12. Influence of microbiota inoculum as a substitute for antibiotic growth promoter during the initial laying phase on productivity performance, egg quality, and the morphology of reproductive organs in laying hens
Bodhi Agustono, Sunaryo Hadi Warsito, Maya Nurwartanti Yunita, Widya Paramita Lokapirnasari, Sri Hidanah, Emy Koestanti Sabdoningrum, Mohammad Anam Al-Arif, Mirni Lamid, Gandul Atik Yuliani, Shekhar Chhetri, and Sarasati Windria
Veterinary World, 16(7): 1461-1467

Bodhi Agustono: Division of Animal Husbandry, School of Health and Life Sciences (SIKIA), Surabaya 60115, Indonesia.
Sunaryo Hadi Warsito: Department of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Maya Nurwartanti Yunita: Division of Pathology Veterinary, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia.
Widya Paramita Lokapirnasari: Department of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Sri Hidanah: Department of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Emy Koestanti Sabdoningrum: Department of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Mohammad Anam Al-Arif: Department of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Mirni Lamid: Department of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Gandul Atik Yuliani: Department of Veterinary Science, Division of Basic Veterinary, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Shekhar Chhetri: Department of Animal Science, Royal University of Bhutan, Thimphu, Bhutan.
Sarasati Windria: Department of Biomedical Science, Faculty of Medicine, Universitas Padjajaran, Bandung, Indonesia.

doi: 10.14202/vetworld.2023.1461-1467

Article history: Received: 24-02-2023, Accepted: 09-06-2023, Published online: 19-07-2023

Corresponding author: Bodhi Agustono

E-mail: bodhiagustono@fkh.unair.ac.id

Citation: Agustono B, Warsito SH, Yunita MN, Lokapirnasari WP, Hidanah S, Sabdoningrum EK, Al-Arif MA, Lamid M, Yuliani GA, Chhetri S, and Windria S (2023) Influence of microbiota inoculum as a substitute for antibiotic growth promoter during the initial laying phase on productivity performance, egg quality, and the morphology of reproductive organs in laying hens, Veterinary World, 16(7): 1461-1467.
Abstract

Background and Aim: Antibiotics that increase growth have long been employed as a component of chicken growth. Long-term, unchecked usage may lead to microbial imbalance, resistance, and immune system suppression. Probiotics are a suitable and secure feed additive that may be provided as a solution. The objective of this research was to ascertain the effects of dietary multistrain probiotics (Lactobacillus acidophilus, Bifidobacterium spp., and Lactobacillus plantarum) on the morphology (length and weight) of reproductive organs and productivity performance of laying hens during the early stage of laying.

Materials and Methods: One hundred ISA Brown commercial layer chicks of the same body weight (BW) that were 5 days old were divided into five treatments, each with four replicates and four chicks in each duplicate. There were five different dietary interventions: (T1) 100% base feed; (T2) base feed with 2.5 g of antibiotic growth promoter/kg feed; (T3) base feed plus probiotics; (T4) base feed at 1 mL/kg with probiotics; and (T5) base feed with probiotics, 3 mL/kg feed, 5 mL/kg of feed. The parameters observed were performance, internal and exterior egg quality, and the morphology (length and weight) of laying hens’ reproductive organs.

Results: Probiotic supplementation (L. acidophilus, Bifidobacterium, and L. plantarum) significantly affected the BW, feed intake, egg weight, yolk index, albumin index, Haugh unit, egg height, egg width, and morphology (length and weight) of laying hens’ reproductive organs compared to the control group (basic feed). In addition, there was no discernible difference between treatment groups in theeggshell weight and thickness variables across all treatment groups.

Conclusion: When laying hens were between 17 and 21 weeks old, during the early laying period, microbiota inoculum supplements (L. acidophilus, Bifidobacterium, and L. plantarum) increased growth, the quality of the internal and external layers’ eggs, and the morphology of the laying hens’ reproductive organs.

Keywords: external quality eggs, good health, growth performance, internal quality eggs, probiotics, reproductive organs.