Open Access
Research (Published online: 24-07-2023)
17. Measurement of serum short-chain fatty acid concentrations in cattle after oral administration of difructose anhydride III
Topas Wicaksono Priyo Jr, Seiichi Uno, Emiko Kokushi, Katsuki Toda, Hiroshi Hasunuma, Daisaku Matsumoto, Osamu Yamato, Masayuki Ohtani, Oky Setyo Widodo, Dhidhi Pambudi, Masayasu Taniguchi, and Mitsuhiro Takagi
Veterinary World, 16(7): 1505-1511

Topas Wicaksono Priyo Jr: Department of Clinical Veterinary Science, Joint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan; Department of Reproduction and Obstetric, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia.
Seiichi Uno: Education and Research Center for Marine Resource and Environment Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan.
Emiko Kokushi: Education and Research Center for Marine Resource and Environment Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan.
Katsuki Toda: Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan.
Hiroshi Hasunuma: Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan.
Daisaku Matsumoto: Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan.
Osamu Yamato: Department of Veterinary Clinical Science, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0062, Japan.
Masayuki Ohtani: Nippon Beet Sugar Manufacturing Co., Ltd., Obihiro 080-0835, Japan.
Oky Setyo Widodo: Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Indonesia.
Dhidhi Pambudi: Department of Mathematics Education, Faculty of Teacher Training and Education, Sebelas Maret University, Indonesia.
Masayasu Taniguchi: Department of Clinical Veterinary Science, Joint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan; Department of Veterinary Clinical Science , Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.
Mitsuhiro Takagi: Department of Clinical Veterinary Science, Joint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan; Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Indonesia; Department of Veterinary Clinical Science , Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

doi: 10.14202/vetworld.2023.1505-1511

Article history: Received: 22-03-2023, Accepted: 14-06-2023, Published online: 24-07-2023

Corresponding author: Mitsuhiro Takagi

E-mail: mtakagi@yamaguchi-u.ac.jp

Citation: Priyo TW Jr., Uno S, Kokushi E, Toda K, Hasunuma H, Matsumoto D, Yamato O, Ohtani M, Widodo OS, Pambudi D, Taniguchi M, and Takagi M (2023) Measurement of serum short-chain fatty acid concentrations in cattle after oral administration of difructose anhydride III, Veterinary World, 16(7): 1505-1511.
Abstract

Background and Aim: We previously reported the mitigation effects of difructose anhydride III (DFA III) on mycotoxins, such as zearalenon and sterigmatocystin, based on the urinary concentrations of these molecules in calves. This study was aimed at evaluating the effects of dietary supplementation of DFA III and the fermented status of DFA III in the intestine by comparing serum levels of short-chain fatty acid (SCFAs) in DFA III-supplemented cattle with those in non-supplemented control cattle.

Materials and Methods: Serum SCFA concentrations were measured in 30 Japanese Black heifers, aged 9–10 months, from two herds, using gas chromatography on days 0 (before DFA III supplementation), 9, and 14 after DFA III supplementation.

Results: A notably different trend was observed for isobutyric acid and enanthic acid, which may reflect the different fermentation status of supplementary DFA III in the intestine. Our results indicate the possibility that this trend observed in the intestinal tract following DFA III administration is associated with changes in the environment of intestinal bacterial flora, which may partially reflect the effects of DFA III supplementation on cattle.

Conclusion: Difructose anhydride III supplementation for at least 2 weeks affects the trend of blood SCFA concentrations in cattle. Our results provide evidence supporting the effects of DFA III on the intestinal environment and intestinal barrier function.

Keywords: cattle, difructose anhydride III, short-chain fatty acid.