Vet World   Vol.16   July-2023  Article-17

Research Article

Veterinary World, 16(7): 1505-1511

https://doi.org/10.14202/vetworld.2023.1505-1511

Measurement of serum short-chain fatty acid concentrations in cattle after oral administration of difructose anhydride III

Topas Wicaksono Priyo Jr1,2, Seiichi Uno3, Emiko Kokushi3, Katsuki Toda4, Hiroshi Hasunuma4, Daisaku Matsumoto4, Osamu Yamato5, Masayuki Ohtani6, Oky Setyo Widodo7, Dhidhi Pambudi8, Masayasu Taniguchi1,9, and Mitsuhiro Takagi1,7,9
1. Department of Clinical Veterinary Science, Joint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.
2. Department of Reproduction and Obstetric, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia.
3. Education and Research Center for Marine Resource and Environment Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan.
4. Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan.
5. Department of Veterinary Clinical Science, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0062, Japan.
6. Nippon Beet Sugar Manufacturing Co., Ltd., Obihiro 080-0835, Japan.
7. Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Indonesia.
8. Department of Mathematics Education, Faculty of Teacher Training and Education, Sebelas Maret University, Indonesia.
9. Department of Veterinary Clinical Science , Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.

Background and Aim: We previously reported the mitigation effects of difructose anhydride III (DFA III) on mycotoxins, such as zearalenon and sterigmatocystin, based on the urinary concentrations of these molecules in calves. This study was aimed at evaluating the effects of dietary supplementation of DFA III and the fermented status of DFA III in the intestine by comparing serum levels of short-chain fatty acid (SCFAs) in DFA III-supplemented cattle with those in non-supplemented control cattle.

Materials and Methods: Serum SCFA concentrations were measured in 30 Japanese Black heifers, aged 9–10 months, from two herds, using gas chromatography on days 0 (before DFA III supplementation), 9, and 14 after DFA III supplementation.

Results: A notably different trend was observed for isobutyric acid and enanthic acid, which may reflect the different fermentation status of supplementary DFA III in the intestine. Our results indicate the possibility that this trend observed in the intestinal tract following DFA III administration is associated with changes in the environment of intestinal bacterial flora, which may partially reflect the effects of DFA III supplementation on cattle.

Conclusion: Difructose anhydride III supplementation for at least 2 weeks affects the trend of blood SCFA concentrations in cattle. Our results provide evidence supporting the effects of DFA III on the intestinal environment and intestinal barrier function. Keywords: cattle, difructose anhydride III, short-chain fatty acid.

Keywords: cattle, difructose anhydride III, short-chain fatty acid.

How to cite this article: Priyo TW Jr., Uno S, Kokushi E, Toda K, Hasunuma H, Matsumoto D, Yamato O, Ohtani M, Widodo OS, Pambudi D, Taniguchi M, and Takagi M (2023) Measurement of serum short-chain fatty acid concentrations in cattle after oral administration of difructose anhydride III, Veterinary World, 16(7): 1505-1511.

Received: 22-03-2023  Accepted: 14-06-2023     Published online: 24-07-2023

Corresponding author: Mitsuhiro Takagi   E-mail: mtakagi@yamaguchi-u.ac.jp

DOI: 10.14202/vetworld.2023.1505-1511

Copyright: Priyo TW Jr., et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.