Molecular detection of Bartonella and Borrelia in pet dogs in Metro Manila and Laguna, Philippines

Vet World Vol.16 July-2023 Article-23

Research Article

Veterinary World, 16(7): 1546-1551

https://doi.org/10.14202/vetworld.2023.1546-1551

Chae Eun Lee¹, Jeong Hee Ikeda¹, Mikaella Andrea M. Manongdo¹, Dan Rica T. Romerosa¹, Kristina Andrea C. Sandalo-De Ramos¹, Tetsuya Tanaka², and Remil L. Galay¹

1. Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna 4031, Philippines.
2. Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-0065, Japan.

Background and Aim: Bartonella and Borrelia are zoonotic vector-borne pathogens that can infect dogs and humans. Data on Bartonella and Borrelia in dogs in the Philippines are lacking. This study was conducted to validate previous reports and further investigate the occurrence of Bartonella and Borrelia spp. in cities of Metro Manila.

Materials and Methods: A total of 182 canine blood samples were acquired with DNA using a commercial extraction kit from selected veterinary clinics in the cities of Metro Manila and Laguna, Philippines. The mammalian actin was amplified through polymerase chain reaction (PCR), followed by PCR assays targeting Bartonella gltA and Borrelia flaB. Further PCR assays targeting 16S of Borrelia and ospA and ospC of Borrelia burgdorferi were performed for those that showed flaB bands.

Results: A positive band for gltA of Bartonella was observed in 9 (4.95%) samples, whereas a positive band for flaB of Borrelia was observed in 15 (8.24%) samples. Subsequent PCR assays for other genes of Borrelia were negative.

Conclusion: These results confirmed the presence of Bartonella and warranted further investigation for the possible presence of other Borrelia species. Keywords: Bartonella, Borrelia, dogs, polymerase chain reaction, tick-borne pathogens.

Keywords: Bartonella, Borrelia, dogs, polymerase chain reaction, tick-borne pathogens.

How to cite this article: Lee CE, Ikeda JH, Manongdo MAM, Romerosa DRT, Sandalo-De Ramos KAC, Tanaka T, and Galay RL (2023) Molecular detection of Bartonella and Borrelia in pet dogs in Metro Manila and Laguna, Philippines, Veterinary World, 16(7): 1546-1551.

Received: 26-02-2023 Accepted: 12-06-2023 Published online: 31-07-2023

Corresponding author: E-mail: k6199431@kadai.jp and rlgalay@up.edu.ph

DOI: 10.14202/vetworld.2023.1546-1551

Copyright: Lee, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.