Vet World Vol.16 June-2023 Article-10
Research Article
Veterinary World, 16(6): 1252-1259
https://doi.org/10.14202/vetworld.2023.1252-1259
Prototype for rapid test devices to detect residues of sulfonamides in chicken carcasses from traditional breeders in Surabaya, Indonesia
2. Division of Histology, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Rd, 60115, Surabaya, Indonesia.
3. Division of Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Rd, 60115, Surabaya, Indonesia.
Background and Aim: Sulfadiazine, one of the sulfonamide group’s active compounds, is widely used for therapeutic production against several diseases. Veterinary drug residues can have a significant impact on human health conditions. This study aimed to develop a prototype of rapid test devices (RTDs) for detecting sulfadiazine residues on chicken carcasses based on the color indication.
Materials and Methods: Seven samples of carcasses collected from traditional breeders in Surabaya-Indonesia were prepared and tested using RTDs. This sample represents the population considering that in the last report, the use of antibiotics was more than 40%, while the ability to monitor RTDs was estimated at 100. The standard color of purple by Hex code standard color or decimal code color was used to compare the positive samples. A light-emitting diode (LED) lamp was used to observe purple color. Analysis of sulfonamides resulting from RTDs was compared using a ultraviolet-visible spectrophotometer.
Results: Sulfonamides contamination levels of 50% and 100% were detected at concentrations of 0.472 µg/mL and 0.642 µg/mL, respectively. Sulfonamides contamination that was <0.395 µg/mL did not appear purple.
Conclusion: The study’s findings showed that RTDs can be used to detect sulfonamides residues at a limit of detection 0.5 µg/mL after a 45 min exposure to an LED operating at a wavelength of 980 nm (p < 0.05). The limitation of RTDs was not being able to monitor the presence of residues bound in fat samples. Rapid test devices can be developed for commonly monitoring devices due to the limited technology available in the market. Keywords: diazotation, food safety, residues, sulfadiazine, veterinary drugs.
Keywords: diazotation, food safety, residues, sulfadiazine, veterinary drugs.
How to cite this article: Lazuardi M, Hestianah EP, and Restiadi TI (2023) Prototype for rapid test devices to detect residues of sulfonamides in chicken carcasses from traditional breeders in Surabaya, Indonesia, Veterinary World, 16(6): 1252-1259.
Received: 09-02-2023 Accepted: 09-05-2023 Published online: 08-06-2023
Corresponding author: Mochamad Lazuardi E-mail: lazuardi@fkh.unair.ac.id
DOI: 10.14202/vetworld.2023.1252-1259
Copyright: Lazuardi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.