MicroRNA profiling in dogs undergoing induced ischemic heart infarction: An experimental study

Research (Published online: 13-06-2023)

19. MicroRNA profiling in dogs undergoing induced ischemic heart infarction: An experimental study

Liqaa A. Raffee, Khaled Z. Alawneh, Musa Ahmed Mohammed Alshehabat, Hazem Haddad, and Saied A. Jaradat

Veterinary World, 16(6): 1319-1324

Liqaa A. Raffee: Department of Accident and Emergency Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.

Khaled Z. Alawneh: Department of Diagnostic Radiology and Nuclear Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.

Musa Ahmed Mohammed Alshehabat: Department of Clinical Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.

Hazem Haddad: Princess Haya Biotechnology Center, Jordan University of Science and Technology, Irbid 22110, Jordan.

Saied A. Jaradat: Princess Haya Biotechnology Center, Jordan University of Science and Technology, Irbid 22110, Jordan.

doi: 10.14202/vetworld.2023.1319-1324

Article history: Received: 09-12-2022, Accepted: 08-05-2023, Published online: 13-06-2023

Corresponding author: Khaled Z. Alawneh

E-mail: kzalawneh0@just.edu.jo

Citation: Raffee LA, Alawneh KZ, Alshehabat MAM, Haddad H, and Jaradat SA (2023) MicroRNA profiling in dogs undergoing induced ischemic heart infarction: An experimental study, Veterinary World, 16(6): 1319-1324.

Abstract

Background and Aim: MicroRNAs (miRNAs) play an important role in various biological functions. According to many studies, miRNA expression is tissue-specific, strongly controlled throughout embryogenesis, and over- or under-expressed in numerous disorders, including cardiovascular pathologies. This study aimed to screen, characterize, and profile many induced biomarkers (miRNAs) in dog serum before and after experimentally inducing a regional myocardial infarction (MI) by occluding the coronary arteries under general anesthesia.

Materials and Methods: A preclinical experimental animal study recruited 12 healthy canine dogs. The selected canine dogs were anesthetized with 1 mg/kg xylazine and 15 mg/kg ketamine before undergoing femoral arterial catheterization under fluoroscopic supervision. Commercial assay kits were used to purify total RNA and miRNA before the occlusion and 2 h after the occlusion according to the manufacturer’s guidelines, and the samples were stored in RNase/DNase-free water at –80°C. Data were analyzed by GraphPad Prism 5.0 software (GraphPad Prism, San Diego, CA) SPSS, and GenEx software (www.multid.se) or (REST V3).

Results: Among 325 transcribed genes, 20 were identified in 2 h. After MI, 14 biomarkers were negative, indicating downregulation, and 6 (3-F08, 3-B10, 4-A11, 1-A06, 2-E01, 3-F10) were positive, indicating upregulation. Polymerase chain reaction assay results showed a normalized fold-change in gene expression in the test sample. Fold values >1 represented a biologically significant change.

Conclusion: Profiling of miRNAs before and after MI in a dog model revealed upregulation of six previously unidentified biomarkers (3-F08, 3-B10, 4-A11, 1-A06, 2-E01, and 3-F10), indicating various miRNA regulatory patterns.

Keywords: dog model, heart infarction, microRNAs, veterinary.