MicroRNA profiling in dogs undergoing induced ischemic heart infarction: An experimental study

Vet World Vol.16 June-2023 Article-19

Research Article

Veterinary World, 16(6): 1319-1324

https://doi.org/10.14202/vetworld.2023.1319-1324

Liqaa A. Raffee¹, Khaled Z. Alawneh², Musa Ahmed Mohammed Alshehabat³, Hazem Haddad⁴, and Saied A. Jaradat⁴

1. Department of Accident and Emergency Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
2. Department of Diagnostic Radiology and Nuclear Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
3. Department of Clinical Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.
4. Princess Haya Biotechnology Center, Jordan University of Science and Technology, Irbid 22110, Jordan.

Background and Aim: MicroRNAs (miRNAs) play an important role in various biological functions. According to many studies, miRNA expression is tissue-specific, strongly controlled throughout embryogenesis, and over- or under-expressed in numerous disorders, including cardiovascular pathologies. This study aimed to screen, characterize, and profile many induced biomarkers (miRNAs) in dog serum before and after experimentally inducing a regional myocardial infarction (MI) by occluding the coronary arteries under general anesthesia.

Materials and Methods: A preclinical experimental animal study recruited 12 healthy canine dogs. The selected canine dogs were anesthetized with 1 mg/kg xylazine and 15 mg/kg ketamine before undergoing femoral arterial catheterization under fluoroscopic supervision. Commercial assay kits were used to purify total RNA and miRNA before the occlusion and 2 h after the occlusion according to the manufacturer’s guidelines, and the samples were stored in RNase/DNase-free water at –80°C. Data were analyzed by GraphPad Prism 5.0 software (GraphPad Prism, San Diego, CA) SPSS, and GenEx software (www.multid.se) or (REST V3).

Results: Among 325 transcribed genes, 20 were identified in 2 h. After MI, 14 biomarkers were negative, indicating downregulation, and 6 (3-F08, 3-B10, 4-A11, 1-A06, 2-E01, 3-F10) were positive, indicating upregulation. Polymerase chain reaction assay results showed a normalized fold-change in gene expression in the test sample. Fold values >1 represented a biologically significant change.

Conclusion: Profiling of miRNAs before and after MI in a dog model revealed upregulation of six previously unidentified biomarkers (3-F08, 3-B10, 4-A11, 1-A06, 2-E01, and 3-F10), indicating various miRNA regulatory patterns. Keywords: dog model, heart infarction, microRNAs, veterinary.

Keywords: dog model, heart infarction, microRNAs, veterinary.

How to cite this article: Raffee LA, Alawneh KZ, Alshehabat MAM, Haddad H, and Jaradat SA (2023) MicroRNA profiling in dogs undergoing induced ischemic heart infarction: An experimental study, Veterinary World, 16(6): 1319-1324.

Received: 09-12-2022 Accepted: 08-05-2023 Published online: 13-06-2023

Corresponding author: Khaled Z. Alawneh E-mail: kzalawneh0@just.edu.jo

DOI: 10.14202/vetworld.2023.1319-1324

Copyright: Raffee, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.