A parasitological survey and the molecular detection of Entamoeba species in pigs of East Java, Indonesia

Vet World Vol.16 March-2023 Article-27

Research Article

Veterinary World, 16(3): 0-0

https://doi.org/10.14202/vetworld.2023.0-0

Dony Chrismanto^1,2, Nunuk Dyah Retno Lastuti², Makoto Matsubayashi², Lucia Tri Suwanti², Sri Pantja Madyawati², Dyah Ayu Kurniawati², and Fransiska Cecilia Beka¹

1. Department of Animal Science, Faculty of Agriculture, Sriwijaya University, South Sumatra, 30862, Indonesia.
2. Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, 16915, Indonesia.

Background and Aim: In several countries, two Entamoeba porcine species, Entamoeba suis and Entamoeba polecki (subtype 1 and 3), have been detected using molecular methods and identified pathogenicity associated with enteritis. However, globally, Entamoeba infection prevalence in pigs is extremely limited. This study aimed to coprologically and genetically examine pig parasites to estimate prevalence of Entamoeba in three pig farms in East Java, Indonesia.

Materials and Methods: Hundred porcine fecal samples (Landrace) were collected from three East Javan farms in well-known swine industry regions. Fecal samples were examined under microscope after sugar-flotation centrifugation, and molecular species and subtype identification were performed using polymerase chain reaction (PCR) and primer pairs targeting small-subunit ribosomal RNA.

Results: Microscopy examinations identified parasites in 89/100 fecal samples; Entamoeba spp. cysts were the most frequent in these samples. Polymerase chain reaction showed that 58 samples were comprised of mixed Entamoeba suis and Entamoeba polecki, 22 E. suis alone, and nine E. polecki alone infections. Epolec F6–Epolec R6 primers successfully amplified E. polecki ST1–4 subtypes, while Epolecki 1–Epolecki 2 amplified only the E. polecki ST1 subtype. Entamoeba polecki ST1-specific primers successfully detected the ST1 subtype in 19/67 E. polecki positive samples.

Conclusion: Entamoeba spp. prevalence in Indonesian pigs was previously shown to be high. On coprological examination of East Javan pigs, we detected high Entamoeba spp. levels, in which we genetically identified as E. suis (80.0%), E. polecki (67.0%), and E. polecki ST1 (19%). Keywords: East Java, Entamoeba, Indonesia, pig, polymerase chain reaction, small-subunit ribosomal rRNA.

Keywords: East Java, Entamoeba, Indonesia, pig, polymerase chain reaction, small-subunit ribosomal rRNA.

How to cite this article: Chrismanto D, Lastuti NDR, Matsubayashi M, Suwanti LT, Madyawati SP, Kurniawati DA, and Beka FC (2023) A parasitological survey and the molecular detection of

Received: 18-12-2022 Accepted: 09-02-2023 Published online: 27-03-2023

Corresponding author: Nunuk Dyah Retno Lastuti E-mail: nunukdyah53@gmail.com

DOI: 10.14202/vetworld.2023.0-0

Copyright: Chrismanto, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.