Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study

Research (Published online: 07-05-2023)

6. Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study

Arsooth Sanguankiat, Nayika Pinniam, and Phitsanu Tulayakul

Veterinary World, 16(5): 939-945

Arsooth Sanguankiat: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom. 73140, Thailand.

Nayika Pinniam: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom. 73140, Thailand.

Phitsanu Tulayakul: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom. 73140, Thailand.

doi: 10.14202/vetworld.2023.939-945

Article history: Received: 20-12-2022, Accepted: 10-04-2023, Published online: 07-05-2023

Corresponding authors: Phitsanu Tulayakul

E-mail: fvetpnt@ku.ac.th

Citation: Sanguankiat A, Pinniam N, and Tulayakul P (2023) Surveillance of antimicrobial resistance, phenotypic, and genotypic patterns of Salmonella enterica isolated from animal feedstuffs: Annual study, Veterinary World, 16(5): 939-945.

Abstract

Background and Aim: Salmonellosis is a significant foodborne disease that causes serious illness in the gastrointestinal of humans and it is a public health problem worldwide. This study aimed to determine Salmonella spp. in animal feeds, its characteristic, serovar identification, genotyping, and drug sensitivity.

Materials and Methods: Salmonella spp. from animal feedstuffs was collected from January 1 to December 31, 2017, with 657 samples. Serogroup classification was performed by using the slide agglutination test. Then, the samples were analyzed for genotype patterns using pulsed-field gel electrophoresis (PFGE) for DNA fingerprint and antibiotic sensitivity by Vitek^® 2 techniques.

Results: A total of 80 samples (12.17 %) were found to be Salmonella positive; commercial feed compounds of 60 samples (75%); soybean meal of 10 samples (12.5%); pork meal of 5 samples (6.25%); a fish meal of three samples (3.75%) and poultry meal of 2 samples (2.5%). Serogroups B, C, D, and E were found in this study; 8 samples (10%), 39 samples (48.75%), 8 samples (10%), and 13 samples (16.25%), respectively. A total of 12 samples (15%) were not determined by serogrouping. The most common serovars were Salmonella Rissen (10%), Salmonella Mbandaka (8.75%), and Salmonella Livingstone (6.25%), which belong to serogroup C. Nine of eleven pulsotypes were detected when analyzed by PFGE, showed similarity index between 40.8 and 100 %. Antimicrobial susceptibility tests by Vitek^® 2 compact for 11 strains were classified into three groups: resistance to 4, 8, and 11 antibiotics, out of 20 antibiotics.

Conclusion: This study revealed annual variation of Salmonella spp. Serovar, genotype, and phenotype from commercial feed compounds and raw materials of which involved people must be aware.

Keywords: commercial feed compounds, genotype, Salmonella spp., serotype.