Research Article | 19 Nov 2023

Effects of dietary feed supplementation with heat-treated Lactobacillus sakei HS-1 on the health status, blood parameters, and fecal microbes of Japanese Black calves before weaning

Naoya Sasazaki^1,2, Katsuki Toda³, Hiroshi Hasunuma⁴, Daisaku Matsumoto⁵, Urara Shinya⁶, Osamu Yamato^7,8, Takeshi Obi⁹, Takashi Higaki¹⁰, Oky Setyo Widodo^11,12, Kunihiko Ishii¹³, Naoki Igari¹⁴, Daiji Kazami¹⁵, Masayasu Taniguchi^16,17, and Mitsuhiro Takagi^18,12Show more

1. Naoya Sasazaki: Department of Veterinary Medicine , Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.

2. Shepherd Central Livestock Clinic, Akune, Japan.

3. Katsuki Toda: Shepherd Central Livestock Clinic, Akune, Japan.

4. Hiroshi Hasunuma: Shepherd Central Livestock Clinic, Akune, Japan.

5. Daisaku Matsumoto: Shepherd Central Livestock Clinic, Akune, Japan.

6. Urara Shinya: Kagoshima Agriculture Mutual Aid Association, Soo, Japan.

7. Osamu Yamato: Department of Veterinary Medicine , Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.

8. Department of Veterinary Science , Division of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Surabay.

9. Takeshi Obi: Department of Veterinary Medicine , Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.

10. Takashi Higaki: Department of Veterinary Medicine , Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.

11. Oky Setyo Widodo: Department of Veterinary Medicine , Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.

12. Department of Veterinary Science , Division of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga Univers.

13. Kunihiko Ishii: JADD Co., Ltd. Yokohama, Japan.

14. Naoki Igari: Daiwa Pharmaceutical Co., Ltd. Tokyo, Japan.

15. Daiji Kazami: Kazami Food Science, Tochigi, Japan.

16. Masayasu Taniguchi: Department of Veterinary Medicine , Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.

17. Department of Veterinary Medicine , Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.

18. Mitsuhiro Takagi: Department of Veterinary Medicine , Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.

Corresponding author: Mitsuhiro Takagi (mtakagi@yamaguchi-u.ac.jp)

Received: 23-05-2023 , Accepted: 16-10-2023 Published: 2023-11-19

VETERINARY WORLD | pg no. 2293-2302 | Vol. 16, Issue 11 | DOI: 10.14202/vetworld.2023.2293-2302

Citations: —

View PDF

Abstract

Background and Aim: Our previous research suggested that heat-killed Lactobacillus sakei HS-1 (HK-LS HS-1) is potentially beneficial for improving intestinal microbes and reducing the number of medical treatments. This study aimed to investigate the effect of HK-LS HS-1 as a supplement in milk replacers (MRs) on clinical health during the 1-month preweaning period.

Materials and Methods: Eighteen female calves were randomly assigned to either a group receiving the HK-LS HS-1 supplement (n = 9) or a control group without it (n = 9). We then investigated the effect of including supplementary HK-LS HS-1; 0.2% in MRs twice daily at 09:00 and 16:00 on the health, serum biochemical parameters (measured using an automated biochemical analyzer), and fecal bacteriological changes of preweaning Japanese Black calves at the day of the start of supplementation (before HK-LS HS-1 supplementation; day 0), at weaning (day 30), and at 2 weeks (day 45) and 4 weeks (day 60) after weaning.

Results: During the supplementation period (0–30 days), (1) an increase (p = 0.023) was observed in albumin, and there was a tendency of increase in total cholesterol level in the HK-LS HS-1 group but not in the control group; (2) substantial differences were obtained after the weaning period (30–60 days), although no differences were observed from 0–30 days in both groups. The anti-Müllerian hormone (AMH) level was substantially increased after weaning in the control group. No differences were observed in the amounts of Coliform spp. and Staphylococcaceae spp. between the two groups; thus, HK-LS HS-1 supplementation had similar antibacterial effects. A significant reduction was observed in the time to weaning of the HK-LS HS-1 group in the field trial.

Conclusion: Supplementation with HK-LS HS-1 from an early stage after birth to weaning is a cost-effective treatment to improve the growth rate of preweaning calves. However, supplementation during only preweaning periods appears to have no beneficial effects on preventing weaning stress, especially in terms of AMH levels. Keywords: anti-Müllerian hormone, feed supplementation, heat-killed lactic acid bacteria, probiotic, serum amyloid A.

Keywords: anti-Müllerian hormone, feed supplementation, heat-killed lactic acid bacteria, probiotic, serum amyloid A.