Emergence of NDM-1-producing Raoultella ornithinolytica from reservoir water in Northeast Thailand

Vet World Vol.16 November-2023 Article-19

Research Article

Veterinary World, 16(11): 2321-2328

https://doi.org/10.14202/vetworld.2023.2321-2328

Chutima Karnmongkol¹, Piyachat Wiriyaampaiwong¹, Mullika Teerakul¹, Jukkarin Treeinthong², Nattapong Srisamoot¹, and Anupong Tankrathok^1,3

1. Department of Biotechnology, Faculty of Agricultural Technology, Kalasin University, Kalasin, Thailand.
2. Department of Fisheries Technology, Faculty of Agricultural Technology, Kalasin University, Kalasin, Thailand.
3. Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.

Background and Aim: Antibiotic resistance is a major global health threat. The increasing prevalence of drug-resistant bacteria poses a serious challenge to the effective treatment of infections in both humans and animals. Water is a major source of human and animal exposure to bacteria, and the presence of drug-resistant bacteria in water could present a severe threat to public health and animal production. This study investigated the presence of drug-resistant bacteria in Lam Pao Dam (LPD) water in Kalasin, Thailand.

Materials and Methods: Ampicillin-resistant strains were obtained from LPD water and identified using 16s rDNA sequencing. Antibiotic resistance genes were detected by polymerase chain reaction using specific primers. The presence of antibiotic-resistant bacteria was evaluated using 16s amplicon analysis. The minimum inhibitory concentration (MIC) of Raoultella ornithinolytica strains against antibiotics was determined.

Results: A total of 12 R. ornithinolytica, 4 Bacillus cereus, and 4 Enterococcus faecalis isolates were resistant to ampicillin. Almost all R. ornithinolytica strains harbored blaSHV and blaOXA genes, and two strains also harbored the blaNDM-1 gene. All four E. faecalis strains harbored the blaIMP gene. The most abundant species in the LPD sample was Exiguobacterium indicum, followed by E. faecalis and R. ornithinolytica. The MICs of 10 R. ornithinolytica strains against five antibiotics revealed that all strains were resistant to ampicillin but susceptible to meropenem, doripenem, ertapenem, and imipenem.

Conclusion: These findings suggest a high prevalence of drug-resistant bacteria in LPD water. This is a cause for concern, as it could spread antibiotic-resistant infections in the community. Keywords: antibiotic resistance, carbapenemase, Raoultella ornithinolytica, waterborne pathogens.

Keywords: antibiotic resistance, carbapenemase, Raoultella ornithinolytica, waterborne pathogens.

How to cite this article: Karnmongkol C, Wiriyaampaiwong P, Teerakul M, Treeinthong J, Srisamoot N, and Tankrathok A (2023) Emergence of NDM-1-producing Raoultella ornithinolytica from reservoir water in Northeast Thailand, Veterinary World, 16(11): 2321-2328.

Received: 02-08-2023 Accepted: 23-10-2023 Published online: 27-11-2023

Corresponding author: Anupong Tankrathok E-mail: anupong2.ta@ksu.ac.th

DOI: 10.14202/vetworld.2023.2321-2328

Copyright: Karnmongkol, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.