Vet World Vol.16 November-2023 Article-5
Research Article
Veterinary World, 16(11): 2210-2216
https://doi.org/10.14202/vetworld.2023.2210-2216
First report of phenotypic and genotypic (blaOXA-61) beta-lactam resistance in Campylobacter jejuni from broilers in Indonesia
2. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
3. Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia.
4. Bacteriology Laboratory, Balai Besar Veteriner Wates, Jl. Yogyakarta-Wates No. Km. 27, Wates, Yogyakarta 55651, Central Java, Indonesia.
5. Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Enugu - Abakaliki Rd, 481101, Abakaliki, Ebonyi, Nigeria.
Background and Aim: Campylobacter is a zoonotic bacterium that is a major source of foodborne diseases. In humans, most cases of campylobacteriosis are caused by Campylobacter jejuni. Poultry is the main reservoir of Campylobacter for humans, because Campylobacter is part of the normal flora of the digestive tract of poultry. Antimicrobial resistance to several antibiotics in Campylobacter isolated from humans and food animals has increased rapidly. Beta-lactam is an antibiotic with a high prevalence of resistance in Campylobacter. This study aimed to investigate phenotypic and genotypic (blaOXA-61) beta-lactam resistance in C. jejuni from broilers in Indonesia.
Materials and Methods: A total of 100 samples of broiler intestinal contents were obtained from 10 broiler farms in Pasuruan Regency, Indonesia. Campylobacter jejuni was identified using conventional and polymerase chain reaction (PCR)-based methods. Phenotypic detection of beta-lactam resistance was performed using an antimicrobial susceptibility test with antibiotic disks of aztreonam, ampicillin, and amoxicillin-clavulanic acid. Genotypic detection by PCR was performed using the blaOXA-61 gene, which encodes beta-lactamase.
Results: Campylobacter jejuni was identified in 23% of the samples. Phenotypically, 100% (23/23) and 73.9% (17/23) C. jejuni isolates had high resistance to aztreonam and ampicillin, respectively, but all isolates were susceptible to amoxicillin–clavulanic acid. Genotypically, all isolates carried blaOXA-61, indicated by the presence of a 372-bp PCR product.
Conclusion: Campylobacter jejuni is highly resistant to beta-lactams and is a serious threat to human health. Resistance to beta-lactams should be monitored because beta-lactamase genes can be transferred between bacteria. Public awareness must also be increased on the importance of using antibiotics rationally in humans and animals. Keywords: antimicrobial resistance, beta-lactam, blaOXA-61, Campylobacter jejuni, human health.
Keywords: antimicrobial resistance, beta-lactam, blaOXA-61, Campylobacter jejuni, human health.
How to cite this article: Yanestria SM, Effendi MH, Tyasningsih W, Mariyono M, and Ugbo EN (2023) First report of phenotypic and genotypic (blaOXA-61) beta-lactam resistance in Campylobacter jejuni from broilers in Indonesia, Veterinary World, 16(11): 2210-2216.
Received: 17-07-2023 Accepted: 03-10-2023 Published online: 02-11-2023
Corresponding author: Mustofa Helmi Effendi E-mail: mhelmieffendi@gmail.com
DOI: 10.14202/vetworld.2023.2210-2216
Copyright: Yanestria, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.