Cisplatin (CP) is a preferred drug for cancer treatment but it has dose-dependent side effects. Vitex agnus-castus (VAC) berry extract has antioxidant, free-radical scavenging, and anti-inflammatory activities. This study explored the mitigating effects of VAC extract (VACE) on acute hematotoxicity induced by CP in female Wistar rats. Female Wistar rats (n = 30) were randomly divided into five groups (n = 6/group). The normal control (NC) group received no treatment. The CP control group received CP (7 mg/kg.b.w. ip, single dose) and the drug control group (VACE-650) received VACE (650 mg/kg b.w. oral, daily) for 7 days. Both groups received a single dose of CP (7 mg/kg b.w. ip), followed by 350 and 650 mg/kg.b.w. of VACE daily orally (CPVACE-350 and CPVACE-650 groups, respectively) for 7 days. After a single dose of CP (7 mg/kg b.w.), the red blood cells (RBC), hematocrit (HCT), white blood cells (WBC), and platelets significantly decreased. In the VAC-350 group, the reduction in total WBC count was less than that in the VAC-650 group on the 3rd day. The RBC and HCT values of the VACE groups were better than that of the CP control, but the VACE-350 treatment group showed significant improvement only on the 3rd day. Our findings showed that VACE can mitigate CP-induced damage to peripheral blood cells at lower doses. Keywords: cisplatin, hematotoxicity, rats, Vitex agnus-castus.

Fungal infections are a growing problem for both humans and animals due to the emergence of pathogenic strains resistant to modern antifungal treatments. To evaluate the efficacy of new antifungal drugs, it is essential to develop animal models that demonstrate typical responses to both the infection (pathogenesis and clinical course) and to the treatment, including adverse effects. In this study, we established a rabbit otitis model by infection of an aggressive multidrug-resistant strain from dogs, Malassezia pachydermatis C23, with no need for concomitant immunosuppression. Twenty healthy adult male gray giant rabbits (1 year old, 5.5 kg) were inoculated once with M. pachydermatis C23 at 108 colony-forming units/mL. We observed the clinical signs of the disease and collected ear smears and blood samples every 5 days. The infection progressed rapidly and exhibited characteristic clinical signs without spontaneous recovery for at least 1 month. In fact, substantial deterioration was observed as evidenced by blood parameters. This rabbit otitis model established using an aggressive drug-resistant fungus strain without immunosuppression could prove valuable for testing novel antifungal agents. Keywords: in vivo model, Malassezia pachydermatis, otitis media, rabbit.

Studies on ticks of public health concern in equine husbandry are scarce in the Northeastern region of Brazil. This study aimed to investigate the presence of ticks on horses in the State of Alagoas, which is classified as a silent area for human rickettsiosis. Ticks infesting horses were collected using anatomical tweezers or a commercial hook and kept in ethanol-labeled tubes for taxonomic identification. A total of 2,238 ticks were found. Ticks were identified as 2,215 (98.89%, 95% CI: 98.41–99.28) Dermacentor nitens, 19 (0.98%, 95% CI: 0.05–1.38) Amblyomma sculptum, and 4 (0.18%; 95% CI: 0.007–0.46) Rhipicephalus microplus. This is the first study to report A. sculptum and D. nitens in the State of Alagoas. The presence of A. sculptum should draw the attention of public health managers once Alagoas State is considered a silent area for rickettsial diseases, which means the absence of local surveillance programs for these pathogens. Keywords: Amblyomma sculptum, public health, silent area.

Automated pig feeding system is an emerging technology with the potential to considerably enhance pig farming. This study aimed to explore the benefits of automated pig feeding systems and provide a simplified cost– benefit analysis, which would serve as a valuable decision-making tool for the stakeholders. This study conducted a literature review of automated pig feeding systems and explored recent advancements. We conducted a cost–benefit analysis to assess the economic feasibility of implementing an automated feeding system in pig farming. Finally, the case study site, a pig farm in Kazakhstan, has been introduced to provide key information. The results described an automated pig feeding system suitable for a farm with 500 pigs in Kazakhstan. The case study was further enhanced using a simplified cost–benefit analysis tailored to the farm’s needs and circumstances. The designed automated pig feeding system is a marked advancement that seamlessly integrates the currently available automation and management technologies. Its distinguishing feature is the inclusion of remote control capabilities and real-time data provision, which utilize modern technology to transform pig farming management. Keywords: automated feeding system, cost–benefit analysis, efficiency, pig farming.

Campylobacter is a zoonotic bacterium that is a major source of foodborne diseases. In humans, most cases of campylobacteriosis are caused by Campylobacter jejuni. Poultry is the main reservoir of Campylobacter for humans, because Campylobacter is part of the normal flora of the digestive tract of poultry. Antimicrobial resistance to several antibiotics in Campylobacter isolated from humans and food animals has increased rapidly. Beta-lactam is an antibiotic with a high prevalence of resistance in Campylobacter. This study aimed to investigate phenotypic and genotypic (blaOXA-61) beta-lactam resistance in C. jejuni from broilers in Indonesia. A total of 100 samples of broiler intestinal contents were obtained from 10 broiler farms in Pasuruan Regency, Indonesia. Campylobacter jejuni was identified using conventional and polymerase chain reaction (PCR)-based methods. Phenotypic detection of beta-lactam resistance was performed using an antimicrobial susceptibility test with antibiotic disks of aztreonam, ampicillin, and amoxicillin-clavulanic acid. Genotypic detection by PCR was performed using the blaOXA-61 gene, which encodes beta-lactamase. Campylobacter jejuni was identified in 23% of the samples. Phenotypically, 100% (23/23) and 73.9% (17/23) C. jejuni isolates had high resistance to aztreonam and ampicillin, respectively, but all isolates were susceptible to amoxicillin–clavulanic acid. Genotypically, all isolates carried blaOXA-61, indicated by the presence of a 372-bp PCR product. Campylobacter jejuni is highly resistant to beta-lactams and is a serious threat to human health. Resistance to beta-lactams should be monitored because beta-lactamase genes can be transferred between bacteria. Public awareness must also be increased on the importance of using antibiotics rationally in humans and animals. Keywords: antimicrobial resistance, beta-lactam, blaOXA-61, Campylobacter jejuni, human health.

In Indonesia, the buffalo is important for small and marginal farmers’ livelihood and economic development as a source of food, working animal, and tourist attraction. Therefore, an in-depth study is needed to examine challenges and opportunities for buffalo development in Indonesia. In Indonesia, the buffalo is divided into two types: swamp buffalo and river buffalo. The buffalo population in Indonesia has declined significantly. A decrease of approximately 39.35% was recorded from 2022 to 2017. The decline occurred due to low reproduction rate and suboptimal rearing management systems. There are three buffalo-rearing systems: Intensive, semi-intensive, and extensive. The productivity of buffalo is diverse and closely related to the characteristics of the regional agroecosystem, consistent with existing natural resources and rearing management systems. The diversity of buffalo productivity provides a good opportunity to improve productivity. Improvement of buffalo genetics is urgently needed, by improving mating management, etc., especially to reduce potential inbreeding. In recent years, genetic and molecular research on Indonesian buffalo has made progress, including use of molecular markers, such as microsatellites and single-nucleotide polymorphisms, to evaluate genetic diversity within and among buffalo populations across Indonesia. In addition, studies are being conducted on the relationship of genotype mutations that contribute to appearance and phenotypic performance (heat stress, reproduction, behavior, coat color, and production attributes) in buffaloes. Identification of genetic diversity in local buffaloes can be improved using various genetic and genomic techniques. These findings will form a basis for the targeted conservation of local buffaloes in Indonesia. This study aimed to collect information on the genetic resources of the local buffalo, particularly its status and production system and provide recommendations for developing buffalo production in Indonesia.

Milk can introduce antibiotics into the human diet which poses a public health risk. Therefore, a study to determine the tetracycline residue in dairy milk and its health risk assessment is needed. A cross-sectional study was performed to detect tetracycline residues in fresh dairy milk samples collected from the districts of Malang, Boyolali, and Padang Panjang, Indonesia, and to evaluate dietary exposure to tetracycline residues through milk consumption in 10-12-year-old children and adults. A total of 203 fresh dairy milk samples were collected from local and smallholder dairy cows in Malang, Boyolali, and Padang Panjang in April and August 2018. High-performance liquid chromatography equipped with a photodiode array at 355 and 368 nm was used to detect tetracycline residues. Data were evaluated for dietary exposure assessment. The results showed that the most common residue found was chlortetracycline (8.37%), followed by tetracycline (7.88%) and oxytetracycline (5.91%) in the concentration range of 14.8-498.4, 11.7-49.4, and 11.6-85.6 ng/g, respectively. Seven (3.45%) samples exceeded the maximum residue limit (MRL) for chlortetracycline. However, neither oxytetracycline nor tetracycline residues exceeded the MRL. The mean concentration of the tetracycline residues was 21.76-137.05 ng/g, resulting in an estimated daily intake of 16.46-172.83 ng/kg body weight/day. Tetracycline residues were found in almost all milk sampling locations. The highest prevalence and residue concentration were obtained from chlortetracycline. Estimated daily intake of tetracycline through milk by 10–12-year-old children and adult consumers was low and the risk to consumers was negligible. Keywords: dietary exposure assessment, fresh dairy milk, high-performance liquid chromatography, residues, tetracycline.

Mechanically recovered meat (MRM) products have been linked to outbreaks of human salmonellosis. However, no studies have investigated the prevalence of Salmonella species in MRM products in South Africa despite the products being imported. Therefore, this study aimed to estimate the prevalence and factors associated with Salmonella spp. contamination of mechanically recovered poultry meat (MRPM) imported into South Africa. This study used secondary data of MRPM consignments imported through a port entry into South Africa from May 2016 to December 2017. Crude and factor-specific proportions of Salmonella positive MRPM and their 95% confidence intervals were calculated. A logistic regression model was used to assess the association among country, season, importer, year, and MRPM Salmonella status. A total of 8127 MRPM consignments were imported. Seventeen percentages (17.18%, 985/5733) of consignments tested positive for Salmonella species and only 364 isolates were serotyped. Salmonella Heidelberg (73.90%, 269/364) was the most common serotype followed by Salmonella Infantis (6.59%, 24/364), Salmonella Salamae (4.67%, 17/364), and Salmonella Schwarzengrund (3.57%, 13/364). The odds of a consignment testing positive for Salmonella spp. was higher among consignments from country-B (Odds Ratio [OR]: 3.958, p < 0.0001) compared to “All others.” The odds of testing positive for Salmonella were also higher among consignments imported in autumn (OR: 1.488, p < 0.0001) but lower among those imported in spring (OR: 0.767, p = 0.0004) and summer (OR: 0.843, p < 0.0001) when compared to the winter season. Consignments imported in 2016 compared to 2017 were 1.563 times (p < 0.0001) as likely to test positive for a Salmonella species. Salmonella species were reported in MRPM consignments in this study with Salmonella Heidelberg being the most common serotype. Furthermore, some Salmonella serotypes reported in this study have been implicated in foodborne disease outbreaks. Country of origin, season, and year of importation were significantly associated with the odds of a consignment testing positive for Salmonella species. Keywords: foodborne, import, mechanically recovered poultry meat, risk factors, Salmonella, zoonoses.

Estrus synchronization of ewes has been accomplished using several protocols with various degrees of success in improving reproductive efficiency and obtaining the most effective protocol used in sheep farming. This study aimed to compare the effectiveness of three treatment protocols and to record the intensity and duration of estrus signs and pregnancy rate in Barbados Black Belly (BBB) sheep. Thirty-two primipara BBB ewes aged 18–24 months were equally divided into three treatment groups. T1: the ewes were injected intramuscularly with 2 mL Lutalyse (PGF2α) (10 mg) on days 0 and 10. T2: 1 mL Fertiline (50 μg; Gonadorelin acetate) on day 0 and 2 mL lutalyse (10 mg) on day 7. T3: 1 mL fertiline (50 μg) on day 0, 2 mL lutalyse (10 mg) on day 7, and 1 mL Fertiline (50 μg) on day 9. Estrus response was assessed using naturally mating rams and ewes. Pregnancy was determined using ultrasonography between 55 and 80 days after the last hormonal injection. The following estrus signs were noted: Swollen vulva, mucus discharge, sniffing, excitement, loss of appetite, mounting, and rapid tail movement. Of the expressed signs, swollen vulva was most frequent, whereas loss of appetite and mucus discharge were the least overt signs recorded. The estrus response (%), onset (%), and duration (h) in ewe synchronization of the three treatment protocols were 100%, 58.3 ± 23.4%, and 48.0 ± 18.2 h (T1), 100%, 61.7 ± 41.2%, and 45.0 ± 27.0 h (T2), and 37.5%, 32.1 ± 1.7%, and 29.2 ± 1.25 h (T3), respectively. The pregnancy rates were 87.5%, 87.5%, 37.5%, and 50.0% in T1, T2, T3, and T4, respectively. Prostaglandin F2α+PGF2α and GnRH+PGF2α synchronization protocols were more effective in the fertilization of BBB ewes with better expression of estrus signs compared with the GnRH+PGF2α+GnRH (OVS) protocol. Keywords: Barbados Black Belly, estrus behavior, estrus synchronization, ewes, Trinidad.

Campylobacter spp. are Gram-negative bacilli that are widely recognized as a primary cause of bacterial gastroenteritis worldwide. Campylobacteriosis is the disease caused by this pathogen. Recently, greater attention has been given to the prevalence of campylobacteriosis in different animals, including pets. These animals are considered to be significant reservoirs for this zoonosis. In Lebanon, the occurrence of Campylobacter infection is high. Our first-of-its-kind pilot study in Lebanon aimed to estimate the fecal prevalence of Campylobacter species in house dogs. Thirty-five rectal swabs were collected from male and female house dogs of different ages, both with or without diarrhea. Samples obtained from the dogs were subjected to qualitative microbiological culture testing and molecular diagnosis by polymerase chain reaction assays after bacterial DNA extraction. Fecal prevalence of Campylobacter spp. in house dogs in this study was 17%. There was a relatively higher prevalence among young females and a significant difference between healthy dogs and those suffering from diarrhea. Campylobacteriosis was found to be prevalent among house dogs in Lebanon, making them potential carriers of Campylobacter species. Keywords: Campylobacter, fecal prevalence, house dogs, Lebanon, polymerase chain reaction.

Caprine arthritis and encephalitis (CAE) is a multisystemic persistent viral disease of goat that causes significant economic losses to the farmers and livestock sector. However, no information in this country is available regarding CAE virus (CAEV) infection. Therefore, this study aimed to estimate the seroprevalence of CAEV infection among the goat population in the selected goat-prone districts in Bangladesh and to identify the associated risk factors of the disease. From July 2021 to June 2022, 446 goat serum samples were randomly collected from the study area. Goat owners were interviewed using a pretested questionnaire to determine the risk factors. A commercial indirect enzyme-linked immunosorbent assay kit was used to screen blood serum for CAEV antibodies. Logistic regression models were used to analyze risk factors and serological data to identify the potential risk factors. Out of 446 serum samples, 19 samples were seropositive against CAEV. The overall seroprevalence was 4.26% (95% confidence interval [CI]: 2.58–6.57). The multivariable logistic regression model identified sex (Female; odds ratio [OR]: 3.98; 95% CI: 1.13–13.95), animal age (12–48 months; OR: 4.93, 95% CI: 0.63–38.13), and biosecurity status (Poor biosecurity; OR: 1.66, 95% CI: 0.46–5.92) as potential risk factors for CAEV seropositivity. This study revealed the serological detection of CAEV in Bangladeshi goats where seroprevalence is found to be relatively low. To eradicate the disease, screening and culling of infected goats from the herd might be implemented. Keywords: Bangladesh, caprine arthritis and encephalitis, enzyme-linked immunosorbent assay, goat, risk factors, seroprevalence.

The breeding of pet animals, especially dogs and cats, in Iraq has increased recently. However, no epidemiological or molecular data exist regarding Giardia duodenalis infection in pet animals, particularly in Babylon Province. Therefore, this study aimed to detect G. duodenalis and its genotypes in pets using microscopic and molecular techniques. For microscopic examination, 150 pet fecal samples (dogs = 75, cats = 75) were examined from October 1, 2022, to March 30, 2023. Fourteen isolates of G. duodenalis (7 per host) were genetically characterized using SSUrDNA gene sequencing. Microscopic examination revealed that the rates of G. duodenalis infection was 14.67% (11/75) and 12% (9/75) in dogs and cats, respectively. According to sex, the infection rate in dogs was 22.22% (8/36) for males and 7.69% (3/39) for females. Conversely, the infection rate in cats was 5.56% (2/36) for males and 17.95% (7/39) for females. The highest infection rates were recorded for animals under 6 months of age, with 16.67% (3/18) for dogs and 27.27% (3/11) for cats. In rural areas, the infection rate was higher than that in urban areas, with 17.65% (6/34) in dogs and 13.89% (5/36) in cats. For the molecular diagnosis, 14 isolates of G. duodenalis (7 per host) were genetically characterized using SSUrDNA gene sequencing. In dogs, the findings revealed specific genotypes, with D at 3/7 (42.86%) and C at 2/7 (28.57%). In addition, zoonotic genotype A was found in 2/7 (28.57%) of dogs. In cats, the specific assemblage F was present in 4/7 (57.14%), while zoonotic genotype A was found in 3/7 (42.86%). This study is considered the first in Babylon Province to detect G. duodenalis genotypes in pet animals (dogs and cats), as some have zoonotic genotypes that could transmit infections to humans. The results of this study illustrate the epidemiological importance of this parasite in this region. Keywords: cat, dog, Giardia duodenalis, microscopic, molecular diagnosis.

Trapped neutrophil syndrome (TNS) is an autosomal recessive genetic disorder found in Border Collies and is characterized by peripheral neutropenia and myeloid hyperplasia of bone marrow. The underlying cause of TNS is associated with a 4-base pair deletion mutation in the vacuolar protein sorting 13 homolog B (VPS13B) gene. In this study, we proposed and validated a novel multiplex allele specific-polymerase chain reaction (MAS-PCR) technique to assess the prevalence of TNS using VPS13B genotypes of Border Collies and Thai Ridgebacks in Thailand. We assessed the prevalence of TNS in 100 Border Collies and 30 Thai Ridgebacks using MAS-PCR-based allelic discrimination technique of the VPS13B gene. We then confirmed the VPS13B genotypes by direct DNA sequencing. A total of 130 samples were successfully genotyped using MAS-PCR assays. Of the two dog breeds examined, the VPS13B mutation was present in Border Collies, whereas Thai Ridgebacks were unaffected by this mutation. In Border Collies, 96% of dogs tested had an intact VPS13B genotype, whereas the remaining individuals had a heterozygous mutation genotype, with prevalence and mutated VPS13B allele frequencies of 4% and 2%, respectively. Using a novel MAS-PCR assay targeting the VPS13B gene, this study demonstrates for the first time that carriers of TNS exist in Border Collies in Thailand. This assay is a reliable and cost-effective tool for diagnosing TNS based on VPS13B genotypes and is suitable for routine clinical practice. Keywords: Border Collie, multiplex allele specific-polymerase chain reaction assay, Thai Ridgeback, trapped neutrophil syndrome, vacuolar protein sorting 13 homolog B genotype.

Echinococcosis is one of the most dangerous parasitic diseases common to humans and animals. In Kazakhstan, echinococcosis is widespread in animals. This study aimed to estimate the prevalence and biological characteristics of echinococcosis in agricultural animals, dogs, wild carnivores, and rodents in the Western region of the Republic of Kazakhstan. The study analyzed slaughtered carcasses of cattle (2500), sheep (4200), pigs (250), horses (91), and camels (45). Furthermore, the study analyzed 144 dogs (herding, rural, and urban), 41 wild carnivores (wolves, red foxes, and corsac foxes), and 339 wild rodents (great gerbils, tamarisk jirds, little ground squirrels, yellow ground squirrels, and muskrats). Postmortem and vital examination methods were used in the helminthological studies of dogs, wild carnivores, and rodents. In agricultural animals, localization and intensity were determined by counting echinococcal cysts in parenchymatous organs. Extensiveness of invasion (EI) averaged 19.2% in cattle, 27.5% in sheep, 5.6% in pigs, and 13.3% in camels. Echinococcal cysts mainly affected the liver in sheep (45.4%) and the lungs in cattle (35.5%). The fertility of echinococcal cysts decreased with age in animals. Acephalocysts were registered mainly in cattle. The highest invasiveness of Echinococcus granulosus was found in herding dogs with an EI of 12.5%. Experimental infestations of dogs showed that maturation of echinococcal eggs occurred by days 36–40 and maturation of segments by day 50. Studies of wild carnivores and rodents showed the presence of E. granulosus (imago stage) in wolves, Alveococcus multilocularis (imago stage) in red foxes and corsac foxes, and A. multilocularis (larval stage) in great gerbils and muskrats. Our data provide evidence of high epizootiological danger for the population and significant damage caused by E. granulosus to animal husbandry in the region. Studies on the spread of echinococcosis suggest the possibility of controlling the situation with human and animal diseases and show the importance of this issue. Keywords: agricultural animals, dogs, Echinococcus, Kazakhstan, rodents, wild carnivores.

Anaplasmosis, a tick-borne disease affecting livestock caused by the bacteria Anaplasma, poses a global concern. This study aimed to estimate the prevalence, spatiotemporal variation, and associated risk factors of anaplasmosis in cattle from the Bannu and Lakki Marwat districts of Khyber Pakhtunkhwa, Pakistan. This study used 197 cattle exhibiting clinical symptoms of anaplasmosis in natural settings. Microscopic examination was used to estimate the prevalence. Potential risk factors, such as sampling regions and months, gender, breed, and age were studied. The study revealed an overall anaplasmosis prevalence of 19.79%. Bannu district exhibited a higher occurrence at 22.10%, compared to Lakki Marwat district at 17.64%. Young cattle (<2 years) demonstrated a notably higher incidence of anaplasmosis (26.78%) compared to adults (>5 years), which had a prevalence of 12.35% (p < 0.05). Female cattle (22.36%) were more susceptible than male cattle (11.11%). Prevalence peaked in June (45.71%) and was lowest in February (3.57%). Crossbred cattle had a higher prevalence (23.52%) than purebred cattle (11.47%). Anaplasmosis can be effectively controlled using a comprehensive approach encompassing selective breeding for resilience, targeted care of young calves and females, effective tick control during warmer months, consistent use of insecticides, and proactive risk factor management. Raising awareness among farmers through diverse channels, including media, is pivotal to bolster tick-borne disease management strategies. Keywords: anaplasmosis, biotic risk factors, cattle, Khyber Pakhtunkhwa, Pakistan, spatiotemporal variation.

Our previous research suggested that heat-killed Lactobacillus sakei HS-1 (HK-LS HS-1) is potentially beneficial for improving intestinal microbes and reducing the number of medical treatments. This study aimed to investigate the effect of HK-LS HS-1 as a supplement in milk replacers (MRs) on clinical health during the 1-month preweaning period. Eighteen female calves were randomly assigned to either a group receiving the HK-LS HS-1 supplement (n = 9) or a control group without it (n = 9). We then investigated the effect of including supplementary HK-LS HS-1; 0.2% in MRs twice daily at 09:00 and 16:00 on the health, serum biochemical parameters (measured using an automated biochemical analyzer), and fecal bacteriological changes of preweaning Japanese Black calves at the day of the start of supplementation (before HK-LS HS-1 supplementation; day 0), at weaning (day 30), and at 2 weeks (day 45) and 4 weeks (day 60) after weaning. During the supplementation period (0–30 days), (1) an increase (p = 0.023) was observed in albumin, and there was a tendency of increase in total cholesterol level in the HK-LS HS-1 group but not in the control group; (2) substantial differences were obtained after the weaning period (30–60 days), although no differences were observed from 0–30 days in both groups. The anti-Müllerian hormone (AMH) level was substantially increased after weaning in the control group. No differences were observed in the amounts of Coliform spp. and Staphylococcaceae spp. between the two groups; thus, HK-LS HS-1 supplementation had similar antibacterial effects. A significant reduction was observed in the time to weaning of the HK-LS HS-1 group in the field trial. Supplementation with HK-LS HS-1 from an early stage after birth to weaning is a cost-effective treatment to improve the growth rate of preweaning calves. However, supplementation during only preweaning periods appears to have no beneficial effects on preventing weaning stress, especially in terms of AMH levels. Keywords: anti-Müllerian hormone, feed supplementation, heat-killed lactic acid bacteria, probiotic, serum amyloid A.

Opisthorchis viverrini infection-induced inflammation contributes to cholangiocarcinoma (CCA) development in humans and animals. Inflammation generates free radicals, such as reactive oxygen species and reactive nitrogen species (RNS), which damage the host’s DNA. However, only 5% of O. viverrini-infected individuals develop malignancy, suggesting that variations in the inflammatory response of individuals to the parasite may influence susceptibility. Due to limitations in studying human susceptibility, we used an animal model to investigate the profiles of inflammatory reactions, oxidative burst, and irreversible DNA damage. This study aimed to explore the potential role of inflammation and RNS in causing DNA damage that may predispose susceptible hosts and non-susceptible animal models to cancer development in O. viverrini infection. This experimental study was conducted on 30 Syrian golden hamsters (OV-H) and 30 BALB/c mice (OV-M) infected with O. viverrini, representing susceptible and non-susceptible models, respectively. Five animals per group were examined at six predetermined time points during the experiment. Biliary tract samples were systematically investigated using histopathological evaluation for inflammatory cell infiltration and immunohistochemical staining for RNS production and markers of DNA damage, including nitrotyrosine and 8-hydroxy-2ʹ-deoxyguanosine. These features were quantified and compared among the experimental groups. Mann–Whitney U-test was used for statistical analysis, with p < 0.05 considered statistically significant. The comparison revealed that the OV-M group exhibited significantly earlier and higher rates of inflammatory cell infiltration during the acute phase, whereas the OV-H group exhibited chronic and more severe inflammation (p < 0.020). Intracellular RNS production and DNA damage were closely associated with the inflammatory response. This study demonstrates differential responses in susceptible and non-susceptible models of O. viverrini infection regarding disease onset and duration, as well as intracellular RNS production and DNA damage caused by inflammation. Persistent inflammation generated oxidatively damaged DNA, which is a distinct pathological characteristic of susceptible hosts and may be critical for CCA development. Keywords: cholangiocarcinoma, DNA damage, inflammatory reactions, Syrian golden hamsters.

Porcine circovirus type 2 (PCV2) is a pathogenic virus that suppresses the immune system of pigs, impacting their health and causing economic losses. Rapid diagnostic tools for early detection of PCV2 are critical to disease prevention and control. Several molecular techniques have been established for detecting PCV2 but costly equipment and time-consuming methods are unsuitable for field inspection. In this study, we developed a recombinase-aided amplification combined with lateral flow dipstick (RAA-LFD) assay to compare with polymerase chain reaction (PCR) and quantitative PCR (qPCR) in detecting PCV2 in suspected field samples. To amplify RAA products, 15 primer pairs were designed from the conserved region of the open reading frame (ORF) 1 gene based on multiple alignments of eight PCV2 genotypes. The most efficient primer pair and conditions for the RAA-LFD assay were tested and selected. Limit of detection, repeatability, and reproducibility were determined using the constructed plasmid. DNA was extracted from positive samples for specificity testing as well as from 100 field samples to compare the detection of the RAA-LFD assay with PCR and qPCR. The F1/R1 primer pair was chosen and labeled with fluorescein isothiocyanate at the 5’ end of the forward primer and with biotin at the 5’ end of the reverse primer. The limit of detection of the RAA-LFD assay was 10 copies/μL at 38°C for 30 min. The RAA-LFD assay was repeatable and reproducible, with no cross-reaction with PCV3, Actinobacillus pleuropneumoniae, Porcine epidemic diarrhea virus, Classical swine fever virus, Porcine reproductive and respiratory syndrome virus - North America strain (PRRSV-US) and Porcine reproductive and respiratory syndrome virus - European strain (PRRSV-EU). Based on testing with 100 samples, the developed RAA showed 100% specificity and 90.56% and 85.71% sensitivity when compared to PCR and qPCR, respectively Cohen’s kappa coefficients showed a good agreement with the established techniques. The RAA-LFD assay targeting the ORF1 gene was highly sensitive, specific, quick, and simple to perform in the field. Keywords: lateral flow dipstick assay, Porcine circovirus type 2, recombinase-aided amplification.

Antibiotic resistance is a major global health threat. The increasing prevalence of drug-resistant bacteria poses a serious challenge to the effective treatment of infections in both humans and animals. Water is a major source of human and animal exposure to bacteria, and the presence of drug-resistant bacteria in water could present a severe threat to public health and animal production. This study investigated the presence of drug-resistant bacteria in Lam Pao Dam (LPD) water in Kalasin, Thailand. Ampicillin-resistant strains were obtained from LPD water and identified using 16s rDNA sequencing. Antibiotic resistance genes were detected by polymerase chain reaction using specific primers. The presence of antibiotic-resistant bacteria was evaluated using 16s amplicon analysis. The minimum inhibitory concentration (MIC) of Raoultella ornithinolytica strains against antibiotics was determined. A total of 12 R. ornithinolytica, 4 Bacillus cereus, and 4 Enterococcus faecalis isolates were resistant to ampicillin. Almost all R. ornithinolytica strains harbored blaSHV and blaOXA genes, and two strains also harbored the blaNDM-1 gene. All four E. faecalis strains harbored the blaIMP gene. The most abundant species in the LPD sample was Exiguobacterium indicum, followed by E. faecalis and R. ornithinolytica. The MICs of 10 R. ornithinolytica strains against five antibiotics revealed that all strains were resistant to ampicillin but susceptible to meropenem, doripenem, ertapenem, and imipenem. These findings suggest a high prevalence of drug-resistant bacteria in LPD water. This is a cause for concern, as it could spread antibiotic-resistant infections in the community. Keywords: antibiotic resistance, carbapenemase, Raoultella ornithinolytica, waterborne pathogens.

Treatment of endometriosis involves pain relief which is achieved through the administration of analgesics and non-steroidal anti-inflammatory drugs, with or without the addition of hormone therapy. At present, studies investigating endometriosis pain management using experimental rat models and the use of medications are scarce. Therefore, this study aimed to systematically evaluate research trends and critical points in the field of endometriosis pain management using experimental models. A total of 30 publications related to this topic that were published from 2012 to 2022 were retrieved from various databases, including Web of Science, Scopus, PubMed, Embase, and CINAHL, using appropriate English keywords. The quality of the publications was evaluated using impact metrics, productivity, term density mapping, and author network. The average publication rate was three articles per year, reaching its peak in 2021 at five articles per year. The United States and China were found to be the most productive countries, with 12 and 10 publications per year, respectively. The field of medicine (37.0%) was the most abundant, although the H-index was relatively low (13:13). Term density mapping involved the search of 542 keywords, of which 35 were selected, with only 8 exhibiting significant density. In the past decade, there has been a shortage of publications that have addressed pain in endometriosis in experimental models and, within this context the majority of the production and publication in this field has been performed by the United States and China. After performing this bibliometric review, it can be inferred that more research is required in this field, to develop new approaches and treatments for endometriotic pain. Keywords: animal model, animal science, endometriosis, rodents, treatments.

Methicillin-resistant Staphylococci (MRS) seriously threatens animal and human health. Repeated antibiotic use allows the bacteria to develop resistance to several antibiotic classes and become multidrug-resistant (MDR). Canine pyoderma, a common skin condition in dogs, is mainly caused by Staphylococci, including MRS. Detecting this infection in all canine populations is crucial to develop a proper preventive plan. This study estimated the prevalence, antibiogram, and risk factors of MRS in canine patients at a referral animal hospital in Khon Kaen, ThailandMethicillin-resistant Staphylococci (MRS) seriously threatens animal and human health. Repeated antibiotic use allows the bacteria to develop resistance to several antibiotic classes and become multidrug-resistant (MDR). Canine pyoderma, a common skin condition in dogs, is mainly caused by Staphylococci, including MRS. Detecting this infection in all canine populations is crucial to develop a proper preventive plan. This study estimated the prevalence, antibiogram, and risk factors of MRS in canine patients at a referral animal hospital in Khon Kaen, Thailand. Skin swabs and relevant information were collected from 56 client-owned dogs that visited the hospital from September 2019 to September 2020. Staphylococci colonies were subjected to molecular identification and antibiotic susceptibility tests using an automated system (VITEK® 2). These colonies were also genetically identified using multiplex-polymerase chain reaction (PCR) and sequencing. The mecA gene, encoding methicillin resistance, was detected using simplex-PCR. The risk factors of MRS infection and their association with MRS infection were analyzed using logistic regression and the Chi-square test, respectively. The prevalence of MRS was found to be 35.7% (20/56 dogs). By species, methicillin-resistant Staphylococcus pseudintermedius was found in 24 of 104 isolates (23.1%), and all samples were MDR. Receiving systemic antibiotics in the past 6 months was a major risk factor associated with MRS infection (p < 0.05; odds ratio (OR) > 1). In addition to the MRS isolates, the mecA gene was also detected in methicillin-susceptible Staphylococci isolates. This might be because of the high expression of blaI, and mutations in c-di-AMP cyclase DacA, RelA, and Fem proteins. A high prevalence of MRS and MDR was observed in the studied population, which might be potentially due to improper antibiotic use by the owners and horizontal transfer of drug-resistance genes. Keywords: antibiogram, methicillin-resistant, prevalence, risk factor, Staphylococci, Thailand.

Working animals are important in agriculture because they play a role in various agricultural activities, including milk and meat production. Thus, they contribute to the development of rural communities. In this study, we aimed to evaluate the effects of different load weights on the work performance and the physiological and hematological responses of working water buffalo (Bubalus bubalis). The work performances of 12 buffaloes (average weight 782.16 ± 21.62 kg) transporting 200, 350, and 500 kg of African palm fruits in metal baskets placed on their backs were evaluated. Work performance variables evaluated immediately after work were as follows: total number of trips (n), total weight (kg), distance traveled (km/day), working period (h), stopped time (h), and average speed (km/h). In addition, we evaluated physiological, biochemical, and hematological variables at three different times: before the start of work, immediately after work, and on the rest day. Among the load weights, 500 kg (total load carried = 4,138.88 kg) improved work efficiency compared to 200 kg loads (total load = 3,322.22 kg) (p = 0.0281). However, 500 kg loads resulted in slower average speed (2.4 km, p = 0.0164), shorter working period (2.39 h, p < 0.0001) and distance traveled (7.29 km, p < 0.0001), and less total number of trips (8.27 trips, p < 0.0001) compared to 350 and 200 kg load weights (3.45 and 3.52 km/h, 2.55 and 2.79 h, 8.71 and 9.75 km, 10.94 and 16.61 trips, respectively); and the heaviest loads resulted in significantly higher (p < 0.005) respiratory rate, pulse, heart rate, rectal temperature, glucose, lactate dehydrogenase, creatine kinase, total protein, white blood cell count, neutrophils, lymphocytes, monocytes, eosinophils, and basophils. In contrast, was associated with lower levels of red blood cells, hemoglobin, and hematocrit compared to lower loads. All differences were more pronounced in 500 kg (p < 0.005) compared to 200 kg loads. Working buffaloes responded to work related to the transport of African palm fruits through various physiological, biochemical, and hematological changes. However, some variables remained close to the reference values reported in the literature for water buffaloes, and in general, all variables were reestablished during the rest day, indicating that these animals have adapted to working conditions. Keywords: biochemical responses, draught buffalo, hematological responses, physiological responses.

An increase in the productivity of broiler chickens is possible when creating an optimal food base that provides birds with all of the nutrients and biologically active substances required for the fullest realization of their genetic potential. In this regard, we examined the effects of the addition of a water-based probiotic and a chelated form of chromium (Cr) to the diet of birds. Sixty 14-day-old male Arbor Acres broilers were used in this study. The birds were assigned to two groups of 30 birds according to their body weights. The control broilers received distilled water with the basal diet, and the experimental group received a probiotic preparation in drinking water and Cr methionine chelate (Cr-Met) in the diet. The feeding period lasted 28 days. Growth performance indices were measured throughout the experiment. At the end of the experiment, blood sampling was performed to assess blood biochemical parameters, antioxidant system indicators, and trace elements. We found that the introduction of a probiotic preparation and a chelated form of Cr into the diet of broiler chickens had a positive effect on meat productivity, which was characterized by a 17% increase in the average daily gain of birds (p = 0.05) and a 14% increase in body weight (p = 0.01). Consequently, the yield of the slaughtered carcass increased by 5.8% (p = 0.05). Against the background of the consumption of the developed diet, broiler chickens exhibited a 14% decrease in feed conversion accompanied by an increase in the level of digestibility of dietary nutrients. In addition, glucose levels were decreased by 20% (p = 0.03) against the background of a 76% increase in the total protein concentration (p = 0.01). Superoxide dismutase and glutathione peroxidase activities were increased by 13% (p = 0.02) and 7.5% (p = 0.03), respectively. Elemental analysis of blood serum revealed a 99% decrease in the Fe level versus the control (p = 0.02) and a 31% increase in the Zn level (p = 0.02). We conclude that feeding broiler chickens is a multicomponent probiotic supplement combined with Cr-Met promotes growth and nutrient absorption, and optimizes metabolic processes. Keywords: antioxidant, broiler chickens, chromium, distilled water.

Asthma, a chronic disease affecting humans and animals, has recently become increasingly prevalent and steadily widespread. The alternative treatment of asthma using helminth infections or helminth-derived immunomodulatory molecules (IMs) has been evaluated and demonstrated significant amelioration of disease severity index in vitro and in vivo. Trichinella spiralis, a parasitic nematode and its IMs, elicits a potential to relieve asthma and other immune-related disorders. In this study, we investigated the immunomodulatory function of recombinant T. spiralis novel cystatin (rTsCstN) in ameliorating acute inflammatory asthma disorders in a murine model. Female BALB/c mice were sensitized using intraperitoneal injection of ovalbumin (OVA)/alum and subsequently challenged with intranasal administration of OVA alone or OVA + rTsCstN for 3 consecutive days, producing OVA-induced allergic asthma models. To evaluate the therapeutic efficacy of rTsCstN, the inflammatory cells and cytokines in bronchoalveolar lavage fluid (BALF) and OVA-specific immunoglobulin E levels in serum were assessed. Histological alterations in the lung tissues were determined by hematoxylin and eosin (H&E) staining and eventually scored for the extent of inflammatory cell infiltration. The asthmatic mouse models challenged with OVA + rTsCstN demonstrated a significant reduction of eosinophils (p < 0.01), macrophages (p < 0.05), and cytokines tumor necrosis factor-α (p < 0.05) and interferon (IFN)-γ (p < 0.05) in BALF when compared with the mice challenged with OVA alone. However, the levels of interleukin (IL)-4 and IL-10 remained unchanged. Histological examination revealed that mice administered OVA + rTsCstN were less likely to have inflammatory cell infiltration in their perivascular and peribronchial lung tissues than those administered OVA alone. Recombinant T. spiralis novel cystatin demonstrated immunomodulatory effects to reduce severe pathogenic alterations in asthma mouse models, encouraging a viable alternative treatment for asthma and other immunoregulatory disorders in humans and animals in the future. Keywords: asthma, immunomodulatory molecule, recombinant Trichinella spiralis novel cystatin, Trichinella spiralis.

African swine fever (ASF), a globally transmitted viral disease caused by ASF virus (ASFV), can severely damage the global trade economy. Laboratory diagnostic methods, including pathogen and serological detection techniques, are currently used to monitor and control ASF. Because the large double-stranded DNA genome of the mature virus particle is wrapped in a membrane, the stability of ASFV and its genome is maintained in most natural environments. This study aimed to investigate the stability of ASFV under different environmental conditions from both genomic and antibody perspectives, and to provide a theoretical basis for the prevention and elimination of ASFV. In this study, we used quantitative real-time polymerase chain reaction for pathogen assays and enzyme-linked immunosorbent assay for serological assays to examine the stability of the ASFV genome and antibody, respectively, under different environmental conditions. The stability of the ASFV genome and antibody under high-temperature conditions depended on the treatment time. In the pH test, the ASFV genome and antibody remained stable in both acidic and alkaline environments. Disinfection tests revealed that the ASFV genome and antibody were susceptible to standard disinfection methods. Collectively, the results demonstrated that the ASFV genome is highly stable in favorable environments but are also susceptible to standard disinfection methods. This study focuses on the stability of the ASFV genome under different conditions and provides various standard disinfection methods for the prevention and control of ASF. Keywords: African swine fever, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, standard disinfection methods, virus stability.

Ear disease is relatively important in veterinary medicine as it significantly affects the quality of life of pets. Two hundred and twenty-one cases of canine ear diseases were collected and collated at the Xi'an Teaching Hospital of Northwest A&F University from 2012 to 2016. An epidemiological analysis was conducted to evaluate the prevalence and causes of various ear diseases in various breeds of dogs in Xi'an. Data were collected and statistically analyzed by reviewing previous cases and obtaining medical history data and laboratory results. This study included the following experiments: systematic examination of the animals' skin, auricular skin scraping test, ear canal endoscopy, and examination of ear canal secretion. The top three dog breeds most commonly afflicted with ear diseases in Xi'an were Toy poodles , Cocker Spaniels, and Golden Retrievers, accounting for 18.5%, 10.4%, and 9.5% of the total cases, respectively. The prevalence was the highest in August and September, with male dogs having a higher prevalence rate than female dogs. Common ear diseases were categorized as otitis externa, otitis media, otitis interna, or ear hematoma. This study determined the prevalence of external otitis, ear hematoma, otitis media, and inner otitis in dogs in the Xi'an area. These results can help expand the current understanding of the development and epidemiology of canine ear diseases and provide a reference for clinical diagnosis, treatment, and prevention. Keywords: dog, epidemiology, otitis externa, Northwest of China.