Leptospirosis, caused by Leptospira spp., affects humans and animals, posing significant health and economic burdens. This multidisciplinary One Health study aimed to estimate the prevalence of Leptospira spp.-specific antibodies in humans and livestock at the human-animal interfaces in Jordan. Study sites were selected across Jordan based on previous research showing a high likelihood of zoonotic pathogen circulation. The study population comprised 500 individuals and 400 livestock (sheep, goats, cattle, and camels). Blood samples were collected from both humans and livestock at baseline and at follow-up after 1.5 years. Humans were followed longitudinally, whereas livestock were sampled opportunistically. The samples were tested for Leptospira spp.-specific Immunoglobulin G antibodies using a commercially available enzyme-linked immunosorbent assay kit. For humans, seroprevalence was calculated from baseline data, and incidence was calculated using follow-up data. In livestock, seroprevalence was calculated at baseline and follow-up. At baseline, the seroprevalence of leptospirosis in humans was 4.0% (95% confidence interval [CI]: 2.5–6.1), with exposure detected across all five sites. The incidence rate of human leptospirosis was 1145 cases/100,000 person-years (95% CI: 455–2,321). In livestock, the baseline seroprevalence was 1.7% (95% CI: 0.79–3.7), which increased to 4.3% (95% CI: 2.6–6.9) at follow-up. Leptospira spp. antibodies were detected only in sheep and goats at both sampling times, with no cases found in camels or cattle. This study highlights the presence and dynamics of leptospirosis at the human-animal interface in Jordan, revealing a baseline human seroprevalence of 4.0% and an incidence rate of 1145 cases per 100,000 person-years. Among livestock, seroprevalence increased from 1.7% to 4.3% over the study period, with exposure limited to sheep and goats. These findings underscore the importance of targeted public health and veterinary interventions to address zoonotic leptospirosis in endemic areas.

Canine parvovirus 2 (CPV-2) is a highly contagious virus that infects wild and domestic canines. Despite the use of a routine vaccination protocol, it is endemic in Iraq. The genetic drift of CPV-2 is a major issue worldwide because it abrogates virus control. In Iraq, there is a knowledge gap regarding the genetic sequences of asymptomatic and symptomatic CPV-2 cases. Therefore, this study aimed to perform a genetic analysis of viral capsid protein 1 (VP1) and viral capsid protein 2 (VP2), two major capsid-encoding genes, to demonstrate the possible role of certain mutations in triggering infection. Symptomatic and asymptomatic cases (n = 100/each) were tested by a polymerase chain reaction targeting VP1 and VP2 genes. The analysis revealed numerous synonymous and nonsynonymous mutations in VP1 and VP2 and in the intergenic sequence. The study identified significant genetic mutations in VP1, VP2, and the intergenic regions of CPV-2 in symptomatic and asymptomatic cases in Iraq. These mutations may contribute to the virus’s ability to evade control measures such as vaccination. These findings indicate that CPV-2 polymorphisms can influence the clinical state of the disease and/or trigger infection. Understanding these genetic variations provides critical insights into CPV-2 pathogenesis and could inform improved vaccination strategies to mitigate the virus’s impact in endemic regions.

Aquaculture, the cultivation of aquatic organisms for human consumption, has become an essential contributor to global food security. However, it faces numerous challenges that threaten its sustainability and capacity to meet the growing demand for animal protein. This review investigates these challenges, with a particular focus on environmental degradation, public health risks, and ethical dilemmas posed by genetic interventions in fish breeding. Despite the promise of genetically modified organisms (GMOs) in enhancing fish production, their integration into aquaculture remains controversial due to potential risks and unresolved ethical questions. This study aims to provide a comprehensive understanding of these pressing issues and propose pathways for sustainable aquaculture development. With the global population increasing and the demand for animal protein intensifying, aquaculture holds great potential as a sustainable food source. However, its contribution to global protein demand remains minimal, projected to decline to as low as 4% in the coming decades. Furthermore, aquaculture’s environmental impact, including pollution of water bodies and ecosystem disruption, poses serious threats to biodiversity and public health. Addressing these challenges is critical for ensuring the long-term viability of aquaculture. By exploring the intersection of sustainability, ethics, and innovation, this review provides valuable insights for policymakers, industry stakeholders, and researchers seeking to advance sustainable aquaculture practices. This study aims to evaluate the current state of aquaculture and identify key challenges related to environmental sustainability, public health, and ethical considerations. It seeks to explore the potential of sustainable practices and genetic interventions to address these challenges while balancing the need for increased production and societal acceptance. The ultimate goal is to offer practical recommendations for fostering a resilient and ethical aquaculture industry capable of meeting future global food demands.

Reproductive traits are crucial for genetic improvement in chickens. Prolactin (PRL) is a gene involved in a complex hormonal network that regulates reproduction in chickens. In this study, three local chicken breeds were crossed and had been produce a second filial (F2) generation, referred to as the F2 local crossed chicken. This study aimed to evaluate reproductive traits, identify single-nucleotide polymorphisms (SNPs), and assess genetic variation in the PRL gene of F2 local crossed chickens. Data on reproductive traits, including body weight (BW) at first egg laying, total egg production (EP) the first 90 days of laying eggs, egg weight (EW), egg length (EL), and egg width (EWd), and blood samples from 60 hens of F2 local crossed chicken (Merawang × Kampung Unggul Balitbangtan [KUB], n= 30; Murung Panggang × KUB [MP × KUB], n= 30) were collected. SNPs within PRL gene were identified using BioEdit version 7.0. Genetic diversity was calculated using Popgen 1.32. Statistical analysis of reproductive traits and its association with genotypes were assessed using Statistical Product and Service Solutions version 20. Crossing patterns had no significant effect on reproductive traits (p > 0.05), except for EWd (p < 0.05). Five polymorphic SNPs were identified in exon 5 of the PRL gene: 8052T>C, 8113G>C, 8187C>T, 8188G>A, and 8321C>T. Observed and expected heterozygosity range from 0.15–0.52 and 0.14–0.38, respectively. All SNPs were in the Hardy-Weinberg equilibrium based on Chi-square test (χ2 <3.841), except for SNP 8052T>C in F2 crossing of MP × KUB. SNP 8052T>C was significantly associated with BW (p < 0.05), with TT genotype chickens showing higher BW. SNP 8187C>T was associated with EW and EL (p < 0.05), with CT genotype chickens having higher values for both traits. This study demonstrates the significant role of the PRL gene in influencing reproductive traits in F2 local crossed chickens. While crossing patterns showed limited impact, specific SNPs in exon 5 of the PRL gene were associated with BW, EW, and EL. The findings highlight PRL gene polymorphisms as valuable markers for improving reproductive traits in poultry breeding programs.

Dairy farm profitability is linked to milk yield and reproductive efficiency. High prolactin levels during lactation can negatively impact fertility. Timed AI protocols like PG7G are used to improve pregnancy rates. This study investigates the effects of extending the voluntary waiting period (VWP) from 63 to 73 days after the PG7G protocol on reproductive parameters, including progesterone and prolactin levels, pregnancy rates, and pregnancy losses, in lactating Holstein cows during summer and winter seasons.  A total of 2100 lactating dairy cows were divided into groups and assigned to the PG7G protocol based on their parity. Two groups were formed based on the number of days in milk (DIM) after the first PGF2α injection. The first group (PG7G-63) received a PGF2α injection 30-day postpartum (pp) and followed a specific protocol. The second group, PG7G-73, followed the same protocol but received a PGF2α injection 40-day pp. Pregnancy was confirmed, and blood samples were collected for analysis. Temperature and humidity were also recorded throughout the study. Extension of the VWP to 73-day (PG7G-73 group) significantly improved pregnancy rates on day 47 pp compared with the 63-day VWP (PG7G-63 group). However, the PG7G-63 group exhibited lower PLs. Seasonality markedly influenced reproductive outcomes, with higher P/AI in the moderate season for primiparous cows and in the hot season for multiparous cows. Conversely, PL in both groups increased during the hot season. Extending the VWP from 63 to 73 days pp in lactating Holstein cows significantly improved pregnancy rates, while seasonality affected reproductive outcomes, with higher pregnancy rates in moderate temperatures and increased pregnancy loss during hot seasons.

The extracted phycocyanin by-products retain nutritional value, including proteins, polysaccharides, and bioactive compounds, which have the potential as feed supplements in broiler production. This study aimed to evaluate the effect of by-products acquired during phycocyanin extraction, which is used as a novel synbiotic supplement, on the production performance and intestinal health of broilers in a tropical climate. A total of 240 one-day-old male Ross 308 broilers were randomly distributed among five dietary treatment groups; they received a diet supplemented with a synbiotic product (probiotic [Lactobacillus johnsonii] at least 1.0 × 108 colony-forming unit/mg with prebiotic [by-product of phycocyanin extraction]) at 0.000%, 0.025%, 0.050%, 0.075%, and 0.100%. We investigated the effects of dietary synbiotic supplements on the growth performance, meat quality, intestinal morphology, and cecal bacterial population of broiler chickens aged 35 days. Synbiotics used as a dietary supplement did not affect the growth performance of broilers during any experimental period (p > 0.05); however, it significantly increased the redness of meat and decreased the levels of thiobarbituric acid-reactive substances on days 3 and 7 of storage (p < 0.05). Moreover, synbiotics significantly improved the height and surface area of villi in the duodenum and jejunum (p < 0.05). The study demonstrated that dietary supplementation with 0.1% synbiotics, incorporating a by-product of phycocyanin extraction, did not significantly influence the growth performance of broiler chickens. However, it positively affected meat quality by increasing redness and reducing lipid oxidation during storage. Additionally, synbiotic supplementation significantly enhanced intestinal health by improving the villi height and surface area in the duodenum and jejunum, highlighting its potential benefits for broiler intestinal morphology and meat quality in tropical climates. Further research is recommended to explore the mechanisms underlying these effects and their implications for long-term poultry health and productivity.

In the past 20 years, acupuncture has been utilized as an alternative therapy for equine laminitis despite a lack of clinical evidence to support its effectiveness. Information from previous studies needs to be evaluated holistically to verify the effectiveness of acupuncture. This meta-analysis aimed to comprehensively investigate the effectiveness of acupuncture as a treatment for laminitis in horses. A total of 7 studies out of 145 were selected in the PubMed, Scopus, Cochrane Library, and ProQuest databases using the keywords “equine laminitis,” “acupuncture,” “horses,” and “lameness score.” Articles were selected following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis flow diagram, and the extracted data were analyzed using OpenMEE software to determine Hedges’ d effect size and Log Odds Ratio. As a result, this meta-analysis study reported that acupuncture improves horses with laminitis (Odds Ratio = 2.254; 95% CI = 1.167–4.355) and has a favorable effect on lameness scores (mean difference = −5.008; 95% CI = −8.094–−1.923). This meta-analysis enhanced the clinical studies demonstrating that twice-weekly acupuncture performed for 4 weeks consecutively can ameliorate lameness scores and a horse’s potential for recovery. These investigations have led to the implementation of dry needling, hemo-acupuncture, aqua-acupuncture, and electroacupuncture as alternate treatments for equine laminitis.

Anaplasmosis in small ruminants is a tick-borne infection caused mainly by the obligate intraerythrocytic bacterium Anaplasma ovis. It is usually subclinical, with persistent infection in affected animals, but acute disease can occur, particularly in young animals. The pathogen is widespread in Central Asia and neighboring regions. In Kazakhstan, the infection was first detected in 1929. However, until now, diagnosis in the country has been based on traditional microscopic examination of blood smears. There were no reliable data on the prevalence and genetic diversity of Anaplasma spp. in sheep in Kazakhstan. This study aimed to determine the occurrence of Anaplasma spp. infection in sheep in southern Kazakhstan, a high-risk region for tick-borne diseases, using PCR and to identify the species by sequencing. A cross-sectional study was conducted on apparently healthy adult ewes from 77 settlements in 34 districts of Kyzylorda, Turkistan, Zhambyl, Almaty, and Jetisu, southern Kazakhstan. A total of 2553 whole blood samples collected in midsummer 2022 and 2023 were analyzed for Anaplasma spp. using polymerase chain reaction targeting the 404 bp groEL gene fragment. The amplification products from the 441 positive samples were sequenced using the Sanger sequencing method. Phylogenetic analysis of the obtained sequences was performed using the maximum likelihood model. Overall, 1017/2553 (39.8%; 95% confidence interval: 37.9%-41.7%) ewes tested were positive for Anaplasma spp. Positive animals were found in 68/77 (88%) of the settlements from which samples were taken. The percentage of Anaplasma spp.-positive ewes varied significantly from 21.3% to 50.1% in the provinces. Altitude <500 m above sea level was identified as a risk factor for Anaplasma infection. All amplification products were identified as A. ovis through sequencing. Phylogenetic analysis of the groEL gene fragment sequences revealed the presence of two A. ovis genotypes; one was 100% identical to sequences from isolates from China and the other was >99.5% identical to isolates from Africa, Cyprus, and China. This first molecular study revealed a widespread of A. ovis infection in adult ewes in southern Kazakhstan. Altitude <500 m was identified as a risk factor. Therefore, clinical cases associated with A. ovis are expected in this region, especially in young animals. Future studies are needed to determine the clinical and economic impact of anaplasmosis on sheep production in the country, to investigate seasonal patterns of infection, and to identify tick species or other arthropods that act as local vectors. This information is useful for developing possible control measures and evaluating their effectiveness.

Lumpy skin disease (LSD) is an economically devastating infectious disease in cattle. Rembang Regency, located in Central Java, Indonesia, has suffered over 3800 cases of LSD and 75 deaths since early 2023. This region holds the 4th number of most populous beef cattle producers in Central Java. However, until now, there have been no reports on the prevalence and risk factors related to LSD in beef cattle in Rembang Regency, Central Java, Indonesia. Therefore, this study aimed to estimate the prevalence of LSD and identify associated risk factors in Rembang Regency, Central Java, Indonesia. The sample size was 458 cattle, which were determined using the formula (n = 4PQ/L2) and two-stage random sampling technique, were examined physically through LSD typical clinical signs, namely distinguishing firm, circumscribed, few (mild forms) to multiple (severe forms) skin nodules. Structured questionnaires and interviews with farm owners were used to identify risk factors. The data related to the LSD were analyzed using descriptive statistics, bivariate analysis with Chi-square and odd ratios, and multivariate logistic regression to retrieve the logit model. All data were compiled in Microsoft® Excel, while analyses were performed using SPSS version 26.0. The prevalence of LSD in Rembang Regency was 28.2%. There were 11 significant risk factors associated with LSD. The multivariate analysis indicated that risk factors significantly contributing to LSD were knowledge of LSD transmission (p = 0.035, Odds ratios [OR] = 2.933), waste management (p = 0.014, OR = 4.015), rainy season (p = 0.019, OR = 2.944), and proximity between farms (p = 0.003, OR = 4.506). The logistic regression model analysis was as follows: LSD (Y) = −6.719 + 1.041 (knowledge of LSD transmission) + 1.390 (waste management) + 1.080 (rainy season) + 1.505 (proximity between farms).  This study revealed a significant prevalence of LSD in Rembang Regency, Central Java, Indonesia, with 28.2% of cattle affected. Key risk factors contributing to LSD outbreaks were insufficient knowledge of transmission, inadequate waste management, seasonal rainfall, and close proximity between farms. These findings emphasize the need for targeted interventions, including educational programs for farm owners, improved waste management practices, and strategies to mitigate seasonal and spatial risks to control LSD in the region effectively.

In the global pig farming industry, artificial insemination (AI) is widely used to propagate high-quality genetics using processed semen that can be stored for extended periods. However, boar sperm are particularly susceptible to oxidative damage during storage and cryopreservation because of their high polyunsaturated fatty acid content. This study aimed to systematically review the impact of resveratrol (RVT), a potent antioxidant, on the preservation of boar semen. A comprehensive meta-analysis was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Relevant articles were retrieved from databases such as Scopus, ScienceDirect, and PubMed using specific keywords. After a thorough screening, 10 studies were selected for inclusion. Data extracted from these studies included parameters such as sperm motility, viability, DNA integrity, and lipid peroxidation levels. Resveratrol supplementation in boar semen extenders significantly improved sperm quality under various storage conditions, including waterbath and cooled and frozen semen. RVT’s antioxidative properties effectively reduced reactive oxygen species and prevented oxidative stress-related damage to sperm cells. The addition of resveratrol to semen extenders enhances the preservation of boar sperm by mitigating oxidative damage, potentially increasing the success rate of AI in the swine industry. This study highlights the need for further research to optimize RVT dosage and application methods for different semen preservation techniques.

The evaluation of the phenotypic and morphological characteristics of indigenous breeds may help to frame breeding policies and plans to implement breed conservation and improvement programs to increase the efficiency of the native breed of the Tarai region. This study aimed to determine the phenotypic and morphological characteristics of indigenous cattle of the Tarai region of North Bihar. A field study was undertaken in 32 villages belonging to 13 blocks of the East and West Champaran districts of Bihar. Animals (n = 562) of different age groups were studied to evaluate their phenotypic and morphological characteristics. It revealed that cattle of the Tarai region are small breeds, and the heights at withers in the females and males were 104.2 ± 0.32 cm and 115 ± 0.27, respectively, with a significant (p = 0.000) difference between sexes. In addition, heart girth was significantly (p = 0.000) higher in males than females. Body length and chest girth were 101.6 ± 0.13 cm and 132.6 ± 0.25 in adult females and 114.8 ± 0.23 cm and 145.7 ± 0.15 cm in adult males, respectively. Body weight also differed significantly (p = 0.001), with 180.9 ± 1.12 and 208.7 ± 1.91 cm for females and males, respectively. Among the draught animals of India, the region cows were comparatively low milkers, with an average lactation yield of 680.2 ± 4.52 kg with a mean lactation length of 224.5 ± 2.06 days and a peak yield of 3.4 ± 0.06 kg/day. The age at first calving and calving interval of Tarai cattle was recorded 32.4 ± 0.22 months and 16.2 ± 0.12 months, respectively. The results of this study could serve as a potential guide for the establishment and identification of new cattle breeds based on the phenotypic and morphometric characteristics of the cattle in Tarai region of North Bihar using baseline data. The data generated from this study can be useful for new Tarai breed identification and serve to establish long-term selective breeding programs for Tarai cattle in the region.

Bali-polled bulls, known for their favorable traits, require advanced reproductive performance analysis to optimize breeding programs. This study aimed to evaluate sperm kinematics and seminal plasma proteomic profiles as biomarkers for sperm motility and fertility in Bali-polled bulls. Semen from five Bali-polled bulls was collected biweekly over five batches using artificial vaginas. Sperm kinematics were assessed using computer-assisted semen analysis (CASA). Fertility was evaluated through service per conception (S/C) in artificial insemination trials. Seminal plasma proteins were analyzed through liquid chromatography–tandem mass spectrometry (LC-MS/MS) and annotated using the UniProt database, PANTHER for gene ontology, and STRING database for protein interactions. Post-thaw sperm kinematics showed satisfactory results, with a mean S/C of 1.52. Proteomic analysis identified 138 proteins, including six (TEX101, BSP1, PRSS55, BSP3, SPADH2, and TPPP2) linked to sperm motility. These proteins were involved in key biological processes such as sperm capacitation, motility regulation, and sperm-oocyte interaction. Sperm kinematics and seminal plasma proteomics provide insights into Bali-polled bull fertility. Identified proteins can serve as fertility biomarkers, aiding in superior local breed development and reproductive efficiency improvement.

Acanthamoeba spp. are pathogenic microorganisms linked to severe infections in humans and animals, requiring a deeper understanding of their encystation process for effective diagnostics and research. This study focused on developing a novel encystment medium to induce synchronized encystation of Acanthamoeba spp. efficiently and rapidly. The study employed response surface methodology with a central composite design to optimize the encystment medium formulation. The key components included Tris-HCl, NaCl, glucose, and MgCl₂. The optimized liquid medium was spray-dried to produce a dehydrated powder for practical application. The encystation efficiency of different Acanthamoeba strains was assessed using hemocytometry and fluorescence microscopy. The optimized medium, comprising 3.152 g/L Tris-HCl, 5.55 g/L NaCl, 8% (w/v) glucose, and 5.0 mM MgCl₂ at pH 9.0, demonstrated exceptional encystation efficiency with rates ranging from 99% to 100%. A spray-dried powdered version of this medium was equally effective, achieving a 98.77% encystation rate for A. castellanii American Type Culture Collection 50739 in glucose-free conditions. Notably, optimal glucose concentrations varied among Acanthamoeba strains, with certain strains reaching maximum encystation at 6–8% glucose. This study successfully developed an innovative encystment medium that promotes rapid and efficient cyst production in Acanthamoeba spp. The medium enhances laboratory research and diagnostic capabilities, paving the way for future advancements in understanding and managing Acanthamoeba infections.

Seminal plasma proteins (SPPs) significantly influence sperm quality, playing a critical role in fertility. This study aims to investigate the molecular weight (MW) profiles of SPPs in horned and polled Bali bulls and their correlation with sperm quality parameters. Semen samples were collected from six Bali bulls (3 horned, 3 polled). Sperm quality was evaluated based on motility, viability, abnormalities, intact membranes, and acrosomes. SPPs were extracted and analyzed using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine protein MWs. Pearson’s correlation was used to analyze relationships between MW profiles and sperm quality metrics. SPPs were identified across a MW range of 15–165 kDa, with specific proteins showing strong correlations with sperm quality. Proteins at 50 and 46 kDa positively correlated with motility (r = –0.96), viability (r = –0.99), and intact membranes (r = –0.86). Conversely, proteins at 40 kDa negatively correlated with these parameters. A 25 kDa protein displayed a positive correlation with intact acrosomes (r = –0.93) and a negative correlation with abnormalities (r = –0.99). Differences in sperm quality metrics between horned and polled bulls were observed, with polled bulls exhibiting fewer abnormalities. This study highlights the potential of SPP MW profiles as biomarkers of sperm quality in Bali bulls. Proteins at 50, 46, and 25 kDa are promising markers for sperm motility, viability, and intact acrosomes, respectively. These findings could inform bull selection and reproductive management strategies. Further research is recommended to validate these biomarkers using advanced proteomic approaches.

The inclusion of Leucaena leucocephala leaf meal (LLM) in poultry feed is often limited due to its high crude fiber and mimosine content. This study investigates the potential of fermented LLM (FLM) to enhance nutrient intake, egg production, and egg quality in laying quails by reducing anti-nutritional factors through fermentation. Two hundred 42-day-old laying quails were assigned to five dietary treatments: T0 (control) = 100% basal diet (BD), T1 = 98% BD + 2% FLM, T2 = 96% BD + 4% FLM, T3 = 94% BD + 6% FLM, and T4 = 92% BD + 8% FLM. Parameters including nutrient intake (energy, protein, fat, calcium, phosphorus), feed conversion ratio (FCR), egg production, egg weight, eggshell thickness, Haugh unit, and yolk color were measured over a 28-day trial. Data were analyzed using a one-way analysis of variance, followed by Duncan’s test for significant differences (p ≤ 0.05). FLM supplementation significantly improved energy, protein, fat, calcium, and phosphorus intake while reducing FCR. At 4% FLM inclusion, significant enhancements in egg production, eggshell thickness, Haugh unit, and yolk color were observed. Conversely, fiber intake and overall feed intake remained unaffected across treatments. The highest egg production (56.43%) and best yolk color (8.95) were observed in the 8% FLM group. Incorporating FLM into the diets of laying quails effectively enhances nutrient utilization and improves egg production and quality without increasing feed intake. The optimal inclusion level for maximizing benefits appears to be 4-8% FLM.

The overuse of antimicrobials in food-producing animals, particularly poultry, has led to growing concerns about multidrug microbial resistance, posing significant risks to both animal and human health. Subtherapeutic doses of antibiotics have traditionally been used to enhance growth and improve economic efficiency in poultry farming. However, these practices have facilitated the emergence of resistant microbial strains, threatening global health security and prompting a search for sustainable alternatives. This review highlights the significance of phytogenic as feed additives (PFAs) as promising substitutes for antibiotic as feed additives (AFAs) in poultry production. PFAs, derived from plant-based compounds, exhibit multiple beneficial properties, including antimicrobial, antioxidative, anti-inflammatory, and immune-modulatory effects. Moreover, they offer the potential to produce high-quality organic poultry products while reducing the likelihood of microbial resistance. Despite these advantages, inconsistent results among studies underscore the importance of standardized approaches to maximize their efficacy. This review aims to evaluate the current status of antibiotic use in poultry farming globally, explore the properties and mechanisms of PFAs, and assess their potential as viable alternatives to antibiotics. By consolidating available knowledge, this review provides insights into the benefits and challenges associated with PFAs, offering guidance for future research and practical applications in sustainable poultry production.

The inclusion of supplementary enzymes, such as phytase and xylanase, in poultry diets enhances nutrient digestibility and reduces excreta output, minimizing environmental impact. This study aimed to evaluate the effects of phytase and xylanase supplementation on laying performance, egg quality, apparent metabolizable energy (AME), and phosphorus utilization in laying hens. A total of 576 laying hens, aged 47 weeks, were randomly assigned to four dietary treatments: (T1) a control diet, (T2) a negative control diet with reduced energy and phosphorus, (T3) T2 supplemented with phytase (50 g/ton) and xylanase (100 g/ton), and (T4) T2 supplemented with phytase and NSP enzyme mix. The study was conducted over 12 weeks. Data on body weight, laying performance, egg quality, AME, and phosphorus utilization were analyzed using a one-way analysis of variance, with statistical significance set at p < 0.05. No significant differences were observed in hen-day egg production, egg weight, or feed intake across groups. However, enzyme supplementation (T3 and T4) significantly reduced feed costs per kg egg and feed cost per egg compared to the control (p < 0.05). Eggshell strength improved significantly in T3 and T4 groups, while yolk color declined in these groups compared to T1 (p < 0.05). Other quality metrics, such as albumen height and Haugh units, showed no significant differences. Apparent metabolizable energy, phosphorus retention time, and feces scores were not significantly affected by dietary treatments. Phytase and xylanase supplementation in diets with reduced nutrient content effectively reduced production costs while maintaining laying performance and improving eggshell strength. These findings support the use of these enzymes as cost-efficient tools in poultry production without compromising egg quality.

Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in dairy farm wastewater represents a significant threat to environmental and public health due to the dissemination of antibiotic-resistance genes. This study investigates the molecular profiles of ESBL-producing E. coli isolates harboring blaCTX-M, blaTEM, and blaSHV genes from dairy farm wastewater in East Java, Indonesia. Wastewater samples (n = 342) were collected from six major dairy regions in East Java: Pasuruan, Malang, Tulungagung, Blitar, Batu, and Kediri. The samples underwent bacterial isolation, identification, and ESBL phenotypic screening using the double-disk synergy test (DDST). Molecular genotyping of bla_CTX-M, bla_TEM, and bla_SHV was conducted using a polymerase chain reaction. Of the samples, 69.30% (237/342) tested positive for E. coli, with 32.91% (78/237) identified as ESBL producers through DDST. Molecular genotyping confirmed ESBL genes in 28.20% (22/78) of the isolates. The genotypic distribution was 77.2% (17/22) bla_TEM, 4.5% (1/22) bla_CTX-M, 9.0% (2/22) bla_TEM + bla_SHV, and 9.0% (2/22) bla_TEM + bla_CTX-M. These findings highlight the dominance of bla_TEM and the presence of multi-gene combinations in East Java’s dairy farm wastewater. The identified molecular profiles underscore dairy farm wastewater as a critical reservoir for antibiotic resistance genes, particularly blaTEM. Addressing this issue necessitates stringent antimicrobial use policies, improved wastewater management, and enhanced biosecurity measures. These findings support a One Health approach to mitigate environmental and public health risks associated with AMR.

The IPB-D2 chicken, a potential disease-resistant local Indonesian line, has been developed to improve poultry health and productivity for small-scale farmers. This study aimed to characterize the disease resistance traits of IPB-D2 chickens by analyzing key immunological parameters, including immunoglobulin Y (IgY) concentration, Newcastle disease (ND) antibody titers, and leukocyte profiles. A total of 100 IPB-D2 chickens were studied. Blood samples from 21-week-old chickens were collected for analysis of IgY concentrations using indirect enzyme-linked immunosorbent assay, ND antibody titers using the hemagglutination inhibition test, and leukocyte profiling using the Giemsa staining method. Correlations between parameters were determined using Pearson’s correlation analysis in RStudio. The findings revealed that IPB-D2 chickens exhibited higher IgY concentrations, leukocyte counts, heterophil levels, and monocyte counts compared to their IPB-D1 progenitors. However, ND antibody titers in IPB-D2 chickens were lower than the protective threshold. Significant positive correlations were observed between leukocytes and lymphocytes, and negative correlations were identified between heterophils and lymphocytes. IPB-D2 chickens demonstrated a well-balanced immune system with enhanced non-specific (leukocyte and heterophil) and specific (IgY) immune responses. These traits highlight their potential as a disease-resistant poultry line. Future research should focus on molecular selection through transcriptomic analysis to identify candidate genes associated with disease resistance traits, enabling further genetic improvements.

Exercise-induced oxidative stress and inflammation adversely affect the health and performance of athletic horses. This study aimed to evaluate the safety of edible bird’s nest (EBN) supplementation and its potential anti-inflammatory and immunomodulatory effects in Arabian race stallions during exercise. Key objectives included assessing EBN’s impact on hepatic and renal function, hematological profiles, and sialic acid (SA) levels. Two experiments were conducted on 18 Arabian stallions. In Experiment 1, six healthy stallions were divided into control (n = 2) and EBN-supplemented groups (n = 4) to assess safety. The treatment group received 10 g of EBN daily for 12 days. Vital signs, hematological parameters, and organ function markers were monitored. In Experiment 2, 12 stallions were divided into three groups: EBN (n = 3), Premier E® (n = 3), and control (n = 6). The exercise regimen included a daily 10-min walk, 10-min canter (30 km/h), and 10-min walk for 30 days. Blood samples were analyzed for hematological indices and SA levels pre- and post-exercise. In Experiment 1, EBN supplementation demonstrated no adverse effects, maintaining normal hematological and vital parameters. Kidney and liver function tests revealed significantly reduced creatine kinase, total bilirubin, and aspartate aminotransferase levels in the EBN group. Experiment 2 showed higher SA levels post-exercise in the EBN group (p < 0.05) compared to Premier E® and control groups, suggesting enhanced anti-inflammatory response. Hematological indices, including mean corpuscular volume, red cell distribution width, and platelet counts, were significantly improved in the EBN group, indicating potential immunomodulatory benefits. EBN supplementation is safe and offers anti-inflammatory and immunomodulatory effects in Arabian race stallions, reducing exercise-induced hepatic and muscular stress markers while enhancing recovery. These findings support EBN as a valuable dietary supplement for performance horses. Further studies should explore long-term effects and molecular mechanisms to optimize its use in equine sports.

Cyanobacterial blooms, driven by anthropogenic and climatic changes, pose significant ecological and health threats. This study investigates the long-term effects of microcystins (MCs), potent cyanotoxins, on cattle at Lake des Oiseaux, a Ramsar-listed wetland in Algeria. Aligning with the “One Health” framework, the research evaluates the biochemical, histological, and oxidative stress impacts of MCs on livestock as environmental sentinels. A herd of 40 cattle (20 exposed and 20 non-exposed) was studied during the summer bloom period of 2019. Blood and liver samples were analyzed to assess biochemical markers (ALT, AST, ALP, GGT, etc.), histopathological changes, and oxidative stress parameters (GPx, CAT, SOD, LPO and GSH). Exposed cattle exhibited significant elevations in liver enzymes and oxidative stress markers, indicating hepatic inflammation and redox imbalance. Histological analysis revealed macrovacuolar steatosis, fibrosis, and bile duct dilatation. Antioxidant enzyme activities (GPx, CAT and SOD) were reduced, with notable depletion of GSH levels and increased lipid peroxidation. These findings reflect the cumulative cytotoxic effects of MC exposure. Non-exposed cattle showed no such changes. Long-term MC exposure disrupts liver function and induces oxidative stress in cattle, implicating significant risks for both animal and human health. The bioaccumulation of cyanotoxins in livestock emphasizes the urgent need for preventive measures, including water monitoring, restricted livestock access to contaminated sites, and farmer education. These strategies are vital to mitigate risks under the “One Health” approach, ensuring sustainable livestock and public health.

Bovine mastitis, an inflammatory condition of the mammary gland, is a critical economic issue in the dairy industry. Antimicrobial resistance (AMR) to mastitis-causing pathogens poses a significant threat to dairy operations in Thailand and Cambodia. This study investigates the AMR of mastitis pathogens in Thailand and Cambodia. It focuses on detecting methicillin-resistant Staphylococcus aureus, vancomycin-resistant S. aureus (VRSA), and vancomycin-resistant enterococci by identifying the presence of mecA, vanA, and vanB genes in bacterial isolates. A total of 65 bacterial isolates (55 S. aureus from Thailand and 10 Enterococcus faecium from Thailand and Cambodia) were analyzed. Disk diffusion tests were conducted for antibiotic susceptibility, and polymerase chain reaction was employed to detect AMR genes. S. aureus isolates showed resistance to penicillin (21.8%), tetracycline (9.1%), and gentamycin (7.3%). Three isolates were identified as multidrug-resistant (MDR), resistant to tetracycline, gentamycin, and penicillin. E. faecium isolates exhibited high resistance to tetracycline (100%) and penicillin (90%), with 60% classified as MDR. Phenotypic analysis identified VRSA in 11% of S. aureus isolates. However, mecA, vanA, and vanB genes were not detected in any isolate. Mastitis pathogens in this study pose significant AMR challenges, especially with MDR S. aureus and E. faecium, and phenotypically VRSA without the vanA gene. The findings highlight the need for judicious antibiotic use in dairy farms and further studies with broader sampling.

Cranial cruciate ligament (CrCL) injuries are a prevalent orthopedic issue in dogs, typically managed through surgical interventions such as tibial plateau leveling osteotomy and tibial tuberosity advancement. However, these techniques have limitations, including high costs and extended recovery periods. This study introduces an innovative CrCL repair method employing an autologous lateral digital extensor muscle tendon graft and evaluates the effects of platelet-rich plasma (PRP) on tissue healing. Twenty-four healthy, male, local-breed dogs were divided into two groups. Group A underwent the surgical procedure without PRP, while Group B received intra-articular PRP during surgery. Outcomes were evaluated through clinical assessments of lameness, post-operative complications, and histological analysis over 10, 20, 30, and 40 days. The PRP-treated group demonstrated statistically significant improvements in post-operative complication scores (p = 0.0025) and histological outcomes (p = 0.0002). However, graft maturation was unaffected by PRP treatment but improved over time (p = 0.0013). PRP-treated dogs exhibited faster recovery and enhanced tissue regeneration, with reduced inflammation and improved graft-bone attachment. This novel surgical approach demonstrates significant potential for improving outcomes in CrCL repair by combining autologous tendon grafting with PRP. The technique offers reduced complications and enhanced healing, providing a promising alternative to traditional methods. Further studies are recommended to validate its efficacy in clinical settings.

Characterizing local animal production systems is crucial for sustainable livestock development. This study aimed to characterize the diversity of goat production systems in the Highlands of Chincha province, Ica, Peru. A structured questionnaire was used to gather data from 82 goat breeders in three districts: San Juan de Yanac, San Pedro de Huacarpana, and Chavín. Factor analysis of mixed data and hierarchical classification analysis were conducted to identify typologies of goat production systems using R version 4.4.2. Four distinct goat production types were identified, primarily differentiated by feeding location and deworming frequency. Type 2 (41.5%) was the most prevalent, characterized by grazing on breeders own land, minimal milk production (<1 liter/day, 91.2%), and a focus on cheese and goat kid sales (70.6%). Breeders were predominantly women, with limited resources and extensive management systems. Across all types, mixed breeding was common, and economic reliance on livestock and agriculture prevailed. Despite their diversity, all goat production systems shared extensive management practices and resource constraints, resulting in low productivity. These findings highlight the need for targeted public policies to improve productivity and sustainability in goat farming within the Ica region.

Prebiotics, such as saccharides in honey, play a crucial role in improving gut microbiota, digestion, and immune function. This study evaluates the effects of Kapok flower honey saccharides on growth performance, digestive enzyme activity, intestinal morphology, and gut microbiota in common carp (Cyprinus carpio). A completely randomized design was implemented with four honey supplementation levels (0% control, 0.5%, 0.75%, and 1%) applied to juvenile C. carpio diets over 30 days. Growth performance, feed utilization, intestinal microvilli structure, gut microbiota, and amylase activity were analyzed using advanced techniques, including high performance liquid chromatography, scanning electron microscopy, and biochemical assays. Kapok flower honey contains fructooligosaccharides (FOS, 14.76%) and inulin (6.6%). Supplementation at 1% significantly improved weight gain, feed conversion ratio, and specific growth rate. Amylase activity increased with honey supplementation, peaking at 24.13 ± 3.11 U g⁻¹ protein for the 1% group. Gut morphology analysis revealed longer, denser intestinal microvilli and higher perimeter ratios in honey-treated groups than controls. Microbiota analysis showed increased beneficial Bacillus spp. exclusively in the honey-supplemented groups. Honey saccharides, particularly FOS and inulin, significantly enhance the growth performance, digestive enzyme activity, and gut health of common carp. Supplementation with 1% honey is optimal, improving feed efficiency and fostering beneficial gut microbiota. These findings highlight honey as a cost-effective, natural prebiotic for aquaculture.

Foot-and-mouth disease (FMD) is a highly contagious viral disease that poses significant economic threats to livestock globally. This study aimed to confirm the presence of FMD virus (FMDV) in Egyptian cattle and identify the predominant serotypes contributing to outbreaks in Sharkia and Dakahlia provinces in 2022. A total of 65 cattle showing acute FMD symptoms were sampled. Desquamated epithelial tissues and oral secretions were analyzed using reverse transcriptase polymerase chain reaction with universal and serotype-specific primers. Seven representative samples underwent sequencing for phylogenetic and genetic variability analysis. All sampled animals tested positive for FMDV. Serotype A accounted for 72.3% of cases, while 27.7% were serotype O. Sequence analysis identified FMDV serotype A (African topotype, genotype IV) and serotype O (East Africa-3 topotype) as the outbreak-causing strains. The identified strains exhibited significant genetic divergence from the vaccine strains used in Egypt, with notable amino acid substitutions in the VP1 protein’s G-H loop. These mutations raise concerns about the efficacy of existing vaccines against current field strains. The study highlights the ongoing threat of FMD in Egypt, particularly among smallholder cattle farmers. The genetic divergence between circulating FMDV and vaccine strains underscores the need to continuously monitor and update vaccine formulations to enhance disease control efforts. Implementing stricter animal movement regulations and tailored vaccination strategies is essential for effective management.

Veterinary World editorial team sincerely like to thank all of our reviewers who contributed to peer review for the journal in 2024.