Vet World Vol.18 September-2025 Article - 25
Research Article
Veterinary World, 18(9): 2867-2877
https://doi.org/10.14202/vetworld.2025.2867-2877
Heavy metal accumulation and its association with epididymal sperm morphometrics in dromedary camels with penile and preputial pathologies
, Ahmed Yassen M. Osman2, Hassan A. Hussein3,4 , Mohammed A. Alqahtani5, Maha Abdullah Momenah6 , Ahmed Ezzat Ahmed5,7 , and Ragab Hassan Mohamed2 1. Department of Animal Productions, Faculty of Agriculture, Al-Azhar University, 71524 Assiut, Egypt.
2. Department of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt.
3. Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
4. Department of Surgery, Obstetrics and Artificial Insemination, Faculty of Veterinary Medicine, Sphinx University, New Assiut, Egypt.
5. Department of Biology, College of Science, King Khalid University, 61413 Abha, Saudi Arabia.
6. Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia.
7. Prince Sultan Bin Abdelaziz for Environmental Research and Natural Resources Sustainability Center, King Khalid University, Abha 61421, Saudi Arabia.
Background and Aim: Sperm morphometry is a vital indicator of fertility, yet male germ cells are highly vulnerable to environmental toxins such as heavy metals. Dromedary camels (DCs) are frequently exposed to penile and preputial pathologies, conditions that can impair semen quality. This study aimed to assess epididymal sperm morphometric characteristics in camels with genital lesions and to explore their associations with concentrations of aluminum (Al), zinc (Zn), molybdenum (Mo), nickel (Ni), and lead (Pb).
Materials and Methods: Sixty adult male DCs (8–10 years, n = 10/group) were classified into six groups: injury-free, balanoposthitis, penile trauma, prolapsed prepuce, phimosis, and penile tumors. Blood samples were analyzed for heavy metal concentrations using inductively coupled plasma mass spectrometry. Epididymal spermatozoa (caput, corpus, cauda) were evaluated for sperm length (SL), sperm head length (SHL), sperm head width (SHW), sperm head length-to-width ratio (SHL/W), and sperm tail length. Sperm abnormalities were categorized into proximal/distal cytoplasmic droplets and tail defects. Data were analyzed by analysis of variance and correlation statistics.
Results: Sperm morphometrics varied significantly with pathological status. Camels with penile and preputial disorders exhibited increased SHW in caudal spermatozoa, while SHL and SHW were reduced in caput spermatozoa (p < 0.01). The phimosis group showed fewer abnormalities and lower total abnormal sperm, whereas the penile tumor group had elevated proximal and distal defects and the highest abnormality rate. Heavy metal analysis revealed significantly increased Zn and Ni concentrations in the penile tumor group, while Mo and Pb levels showed no significant differences. Correlation analysis indicated strong associations of Al and Zn with SHW and SHL/W in caudal spermatozoa, and Ni with SHL, SHW, and SHL/W in corpus spermatozoa (p < 0.05).
Conclusion: Epididymal sperm morphometrics are influenced by penile and preputial pathologies and are significantly associated with Al, Zn, and Ni concentrations. These findings highlight the potential role of heavy metal accumulation in camel reproductive dysfunction and emphasize the need for monitoring environmental exposure in breeding regions.
Keywords: aluminum, dromedary camels, heavy metals, nickel, penile pathology, sperm morphometry, zinc.
How to cite this article: Ali ME, Osman AYM, Hussein HA, Alqahtani MA, Momenah MA, Ahmed AE, and Mohamed RH (2025) Heavy metal accumulation and its association with epididymal sperm morphometrics in dromedary camels with penile and preputial pathologies, Veterinary World, 18(9): 2867-2877.
Received: 21-06-2025 Accepted: 22-08-2025 Published online: 30-09-2025
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2025.2867-2877
Copyright: Ali, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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