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Molecular detection, characterization, and cross-species circulation of feline and canine parvoviruses in Gujarat, India: Emergence of a novel canine parvovirus-2c variant and enhanced diagnostic sensitivity of quantitative polymerase chain reaction

This study aimed to determine the prevalence of FPV and CPV infections among cats and dogs in Gujarat, India, compare the diagnostic performance of rapid antigen testing (RAT), conventional polymerase chain reaction (PCR), and quantitative PCR (qPCR), charact…

Molecular detection, genetic characterization, and phylogenetic relationships of Theileria orientalis in cattle and associated ticks from Wasit Province, Iraq

This study aimed to determine the molecular prevalence of T. orientalis in cattle and their naturally infesting ticks in Wasit Province, Iraq; identify host-associated risk factors; and characterize the genetic diversity and phylogenetic relationships of loca…

Molecular detection and VP1-based phylogenetic characterization of Foot-and-mouth disease virus serotype O circulating in cattle in Aceh, Indonesia

This study aimed to detect FMDV in clinically affected cattle in Aceh, Indonesia, using serological and molecular approaches and to characterize the phylogenetic relationship of the detected virus based on the VP1 gene.

Detection of the iroN virulence gene in multidrug-resistant Escherichia coli isolated from quails in traditional markets of Surabaya, Indonesia

This cross-sectional laboratory-based study aimed to detect MDR E. coli from quail cloacal swabs in Surabaya's traditional markets and screen MDR isolates for the iroN gene, highlighting market level risks within a One Health framework.

Standardized trimodal histopathological examination for microplastic detection and tissue-level assessment in green mussels (Perna viridis) cultivated near an industrial estate in Rayong, Thailand

This study aimed to develop a three-part histopathological exam using light microscopy, polarized light microscopy, and Nile Red (NR) fluorescence, and to analyze MP distribution and linked tissue effects in mussels collected from industrial shoreline areas i…

First molecular insight into Ovar-DRB1 exon 2 in Edilbay sheep: High heterozygosity and detection of novel variants

This study aimed to characterize the diversity of Ovar-DRB1 exon 2 alleles in Edilbay sheep and to identify novel allelic variants using Sanger sequencing.

Development and validation of a regionally adapted sandwich enzyme-linked immunosorbent assay targeting recombinant p60 antigen for rapid detection of Listeria monocytogenes in food samples

This study aimed to develop and validate a regionally adapted sandwich enzyme-linked immunosorbent assay (ELISA) based on monoclonal and polyclonal antibodies (pAbs) raised against a recombinant p60 antigen derived from a Kazakhstani L. monocytogenes field is…

Comparative immunodiagnostic evaluation of recombinant serine proteases from Trichinella spiralis and Trichinella nativa for early detection of trichinellosis

This study aimed to clone, express, and evaluate recombinant serine proteases from Trichinella spiralis (rTsp-LE) to Trichinella nativa (rTnsp-4E), and to assess their diagnostic performance compared with ES antigens and a commercial kit.

Detection and characterization of multidrug-resistant Klebsiella spp. and Enterobacter spp. isolates in Colombian guinea pigs intended for human consumption

This study aimed to detect, characterize, and analyze the AMR patterns and resistance genes of Klebsiella spp. and Enterobacter spp. isolated from slaughtered guinea pigs intended for human consumption in Colombia.

Molecular detection of Shiga toxin-producing Escherichia coli in wild rats from urban areas of Banyuwangi District, Indonesia: Implications for zoonotic risk and public health

This study aimed to detect Shiga toxin-producing E. coli (STEC) in wild rats captured from slum-adjacent urban areas of Banyuwangi District, Indonesia.

Nested open reading frame (ORF) 7 reverse transcription polymerase chain reaction and ORF5 phylogenetic refinement for enhanced detection and genetic classification of porcine reproductive and respiratory syndrome virus-2 in Thailand

This study aimed to (i) establish a nested reverse transcription polymerase chain reaction (RT-PCR) assay targeting the open reading frame 7 (ORF7) gene to detect and differentiate PRRSV-1 and PRRSV-2, and (ii) refine the genetic classification of PRRSV-2 str…

Comparative molecular detection and phylogenetic analysis of Babesia canis vogeli in naturally infected dogs using two 18S rRNA primer sets in Khon Kaen, Thailand

This study aimed to identify and characterize B. canis vogeli in naturally infected dogs in Khon Kaen, Thailand, to compare the diagnostic performance of two primer sets (Bab7/Bab9 and Babf/Babc), and to perform phylogenetic analysis of the isolates.

Development and application of a quantitative polymerase chain reaction assay for the detection and genotyping of bovine leukemia virus in cattle from Kazakhstan

This study aimed to develop a sensitive and specific duplex quantitative polymerase chain reaction (qPCR) assay targeting the env gene of BLV with β-actin as an internal control and apply it for molecular surveillance and genotyping of BLV in cattle from six …

Detection of staphylococcal enterotoxins A and B in cow milk using antigen capture enzyme-linked immunosorbent assay and dot-blot assays

Detection and phylogenetic analysis of Streptobacillus moniliformis, the causative agent of rat-bite fever and Haverhill fever, in free-living greater bandicoot rats in Northeastern India