This study compared whole blood with a pooled BU matrix for quantitative polymerase chain reaction (qPCR) detection of hemotropic and vector-borne pathogens in dogs and cats in Colombia.
Abstract / descriptionThis study compared whole blood with a pooled BU matrix for quantitative polymerase chain reaction (qPCR) detection of hemotropic and vector-borne pathogens in dogs and cats in Colombia.
Abstract / descriptionThe study was undertaken to detect the pathogenic Leptospira in healthy dogs’ urinary shedding by real-time polymerase chain reaction (qPCR)
This study aimed to develop and validate a quadruplex real-time quantitative polymerase chain reaction (qPCR) assay for the simultaneous detection of these four important porcine respiratory bacterial pathogens and evaluate its applicability using clinical sa…
Abstract / descriptionThis study aimed to develop and validate a quadruplex real-time quantitative polymerase chain reaction (qPCR) assay for the simultaneous detection of these four important porcine respiratory bacterial pathogens and evaluate its applicability using clinical samples.
This study aimed to determine the prevalence of FPV and CPV infections among cats and dogs in Gujarat, India, compare the diagnostic performance of rapid antigen testing (RAT), conventional polymerase chain reaction (PCR), and quantitative PCR (qPCR), charact…
Abstract / descriptionThis study aimed to determine the prevalence of FPV and CPV infections among cats and dogs in Gujarat, India, compare the diagnostic performance of rapid antigen testing (RAT), conventional polymerase chain reaction (PCR), and quantitative PCR (qPCR), characterize circulating viral variants through VP2 gene sequencing and phylogenetic analysis, and investigate evidence of cross-species circulation.
This study aimed to develop a sensitive and specific duplex quantitative polymerase chain reaction (qPCR) assay targeting the env gene of BLV with β-actin as an internal control and apply it for molecular surveillance and genotyping of BLV in cattle from six …
Abstract / descriptionThis study aimed to develop a sensitive and specific duplex quantitative polymerase chain reaction (qPCR) assay targeting the env gene of BLV with β-actin as an internal control and apply it for molecular surveillance and genotyping of BLV in cattle from six regions of Kazakhstan.
This study aimed to determine the prevalence of major canine vector-borne pathogens in Bangkok, Thailand during the 2024 rainy season and evaluate the diagnostic performance of microscopy and a novel RNase hybridization-assisted amplification (RHAM) test kit …
Abstract / descriptionThis study aimed to determine the prevalence of major canine vector-borne pathogens in Bangkok, Thailand during the 2024 rainy season and evaluate the diagnostic performance of microscopy and a novel RNase hybridization-assisted amplification (RHAM) test kit in detecting canine Ehrlichiosis, compared to the quantitative polymerase chain reaction (qPCR) gold standard.
In this study, we developed a recombinase-aided amplification combined with lateral flow dipstick (RAA-LFD) assay to compare with polymerase chain reaction (PCR) and quantitative PCR (qPCR) in detecting PCV2 in suspected field samples.
Abstract / descriptionIn this study, we developed a recombinase-aided amplification combined with lateral flow dipstick (RAA-LFD) assay to compare with polymerase chain reaction (PCR) and quantitative PCR (qPCR) in detecting PCV2 in suspected field samples.
The present study was performed for the detection of T. gondii in raw milk samples of goat, sheep, and camel in Upper Egypt using two different techniques ELISA and qPCR
Abstract / descriptionThe present study was performed for the detection of T. gondii in raw milk samples of goat, sheep, and camel in Upper Egypt using two different techniques ELISA and qPCR
Shiga toxin-producing Escherichia coli (STEC) and develop a quantitative polymerase chain reaction (qPCR) assay to quantify the bacterial DNA present in different food matrices
Abstract / descriptionShiga toxin-producing Escherichia coli (STEC) and develop a quantitative polymerase chain reaction (qPCR) assay to quantify the bacterial DNA present in different food matrices